Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate

PurposeTo report the surgical outcome and postoperative hypopigmented change around fovea among patients with high myopia who received human amniotic membrane (hAM) graft transplantation for macular hole (MH).MethodThis retrospective, interventional case series included 10 eyes of 10 consecutive patients (5 (50%) male) with high myopia (axial length over 26.5 mm) who received hAM graft to treat persisted or chronic MH with or without retinal detachment in two hospitals. Postoperative parafoveal atrophy was identified with colour fundus picture and structure optical coherent tomography. Baseline characteristics and short-term visual outcome were analysed.ResultsThe preoperative mean (±SD) axial length and MH diameter were 29.9 (±1.8) mm and 881.8 (±438.5) μm, respectively. After hAM transplantation, seven (70%) eyes had complete MH closure and the mean best-corrected visual acuity (BCVA) improved from 1.26 (±0.48) logarithm of minimal angle of resolution (logMAR) before operation to 1.11 (±0.44) logMAR on the last visit (p=0.074). Patchy atrophy-like depigmentation developed around the MH lesion in four (40.0%) eyes as early as in the first month after surgery. None of them had visual worsening. In terms of demographics, axial length, MH size, ocular history, preoperative BCVA and postoperative BCVA, there was no significant difference between those with and without the parafoveal atrophy. No graft rejection and inflammation happened during the follow-up.ConclusionParafovea atrophy, a rare complication in the conventional MH surgery, was observed in 40% of eyes with highly myopic MH after hAM graft transplantation. The pathogenesis and long-term consequence need further investigations.

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Long-term effects of human amniotic membrane in a rat model of biliary fibrosis

Brazilian Journal of Medical and Biological Research ◽

10.1590/1414-431x20175692 ◽

2017 ◽

Vol 50 (7) ◽

Cited By ~ 6

Author(s):

L.B. Sant'Anna ◽

F.S. Brito ◽

P.R. Barja ◽

M.C. Nicodemo

Keyword(s):

Amniotic Membrane ◽

Rat Model ◽

Human Amniotic Membrane ◽

Long Term Effects

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Long-term follow-up of cryopreserved amniotic membrane transplant during strabismus reoperations: Up 85 months’ follow-up

European Journal of Ophthalmology ◽

10.1177/1120672118757432 ◽

2018 ◽

Vol 28 (4) ◽

pp. 365-371 ◽

Cited By ~ 2

Author(s):

Rehab Rashad Kassem ◽

Ahmed Mostafa Kamal ◽

Randa Mohamed Abdel-Moneim El-Mofty ◽

Hala Mostafa Elhilali

Keyword(s):

Amniotic Membrane ◽

Successful Outcome ◽

Amniotic Membrane Transplantation ◽

Human Amniotic Membrane ◽

Ocular Motility ◽

Long Term Follow Up ◽

A Prospective Study ◽

Acceptable Outcome

Purpose: To evaluate the long-term effect of cryopreserved human amniotic membrane transplant during strabismus reoperations. Methods: A total of 15 patients with persistent strabismus were included in a prospective study to evaluate the effect of wrapping the extra-ocular muscles with cryopreserved amniotic membrane during strabismus reoperations. The study end-point was the last follow-up visit. A successful outcome was defined as 0–10∆ of horizontal tropia and 0–4∆ of vertical tropia, with no limitation of ductions exceeding −1. A cosmetically acceptable outcome was defined as a tropia of 0–15∆. Results: Maximum follow-up was 85 months (mean: 25.4 ± 25.5 months). At the last follow-up visit, a successful outcome was achieved in 46.7%, a cosmetically acceptable outcome was achieved in 66.7%, and the mean ocular deviation angles improved from 38.60 ± 14.63∆, preoperatively, to 10.6 ± 11.08∆. Motility limitation on the final follow-up visit exceeded −1 in only 4/180 muscles (2.2%). Conclusion: The effect of cryopreserved amniotic membrane transplantation on the success of strabismus reoperations was moderate in terms of ocular alignment. Its effect was more pronounced in terms of ocular motility. The latter better reflects the level of adhesions. No long-term complications were documented, denoting safety of cryopreserved amniotic membrane usage during strabismus reoperations.

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The clinical effectiveness of cryopreserved human amniotic membrane in diabetic foot syndrome

Cell and Organ Transplantology ◽

10.22494/cot.v9i2.129 ◽

2021 ◽

Vol 9 (2) ◽

Author(s):

A. Ustymenko ◽

◽

P. Nemtinov ◽

S. Bolgarska ◽

L. Zaika ◽

...

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Amniotic Membrane ◽

Diabetic Foot ◽

Diabetic Foot Syndrome ◽

Human Amniotic Membrane ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Patients With Diabetes

Diabetic foot syndrome with long-term unhealed wounds is the most common complication and cause of limb amputation in diabetes. The search for effective therapeutic agents and their inclusion in treatment protocols is a priority due to the increase in the number of cases of this socially significant disease and disability among the working population every year. The aim of the study is to evaluate the effectiveness of cryopreserved human amniotic membrane in the treatment of long-term non-healing wounds of the lower extremities in diabetic foot syndrome. Materials and methods. The pilot clinical study described 4 clinical cases of treatment of patients with diabetes mellitus type I and II (1 woman and 3 men aged 52 to 68 years) with long-term unhealed wounds of the limbs under standard therapy. After previous wound sanation the applications of the cryopreserved human amniotic membrane were performed. Once a week after the application, the dynamics of wound healing was assessed. Blood glucose levels were determined before amniotic membrane treatment and two hours after the procedure. Results. As a results of weekly applications of human amniotic membrane there was a gradual decrease in the area of the wound from the original size and increase the rate of healing. Thus, at the time of the second visit (after 7 days) the reduction in the area of the ulcer from the initial size in patient 1 was 33 %, patient 2 – 25 %, patient 3 – 33 % on the sole and patient 4 – 3 %, and the healing rate – 4.7 %, 3.6 %, 4.7 % and 0.43 % per day, respectively. The use of human amniotic membrane did not affect blood glucose levels when comparing values before application and two hours after the procedure. Regular follow-up visits of patients 3, 6, 9 and 12 months after the start of the study showed no recurrence of ulcers. Conclusions. It has been shown that the use of cryopreserved human amniotic membrane in patients with diabetes mellitus and diabetic foot syndrome with long-term unhealed wounds results in complete healing of ulcers with stable remission during the year of observation.

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