PCR examination of bronchoalveolar lavage samples is a useful tool in pre-clinical diagnosis of ovine pulmonary adenocarcinoma (Jaagsiekte)

2007 ◽  
Vol 83 (3) ◽  
pp. 419-427 ◽  
Author(s):  
K. Voigt ◽  
M. Brügmann ◽  
K. Huber ◽  
P. Dewar ◽  
C. Cousens ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Clinical Diagnosis ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma

Evaluation of the efficacy of clinical diagnosis of ovine pulmonary adenocarcinoma

2008 ◽  
Vol 162 (3) ◽  
pp. 88-89 ◽  
Author(s):  
C. Cousens ◽  
M. Graham ◽  
J. Sales ◽  
M. P. Dagleish
Keyword(s):  
Clinical Diagnosis ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma

Imaging and score-based quantification of ovine pulmonary adenocarcinoma using computed tomography as an additional tool in advanced clinical diagnosis

2011 ◽  
Vol 96 (2-3) ◽  
pp. 201-210 ◽  
Author(s):  
Esther Humann-Ziehank ◽  
Carsten Brauer ◽  
Ants Kuks ◽  
Arnim Andreae ◽  
Michael Ludwig Bruegmann ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Clinical Diagnosis ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Additional Tool

scholarly journals Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

2016 ◽  
Vol 60 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Aliasghar Bahari ◽  
Masoud Sabouri Ghannad ◽  
Omid Dezfoulian ◽  
Fereydon Rezazadeh ◽  
Ali Sadeghi-Nasab
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mediastinal Lymph Node ◽  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Jaagsiekte Sheep Retrovirus ◽  
Tissue Samples ◽  
Blood Samples ◽  
Proviral Dna ◽  
Healthy Sheep

Abstract Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

T Lymphocytosis in a Bronchoalveolar Lavage of a Pulmonary Adenocarcinoma: Case Report

Respiration ◽  
1990 ◽  
Vol 57 (1) ◽  
pp. 57-61 ◽  
Author(s):  
T.C.M.T van Maarsseveen ◽  
J. Stam ◽  
J.J. Calame
Keyword(s):  
Case Report ◽  
Bronchoalveolar Lavage ◽  
Pulmonary Adenocarcinoma

scholarly journals Gene Expression Profiling of Bronchoalveolar Lavage Cells Preceding a Clinical Diagnosis of Chronic Lung Allograft Dysfunction

2017 ◽  
Vol 14 (Supplement_3) ◽  
pp. S252-S252 ◽  
Author(s):  
S. Samuel Weigt ◽  
Xiaoyan Wang ◽  
Vyacheslav Palchevskiy ◽  
Naman Patel ◽  
Ariss Derhovanessian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bronchoalveolar Lavage ◽  
Gene Expression Profiling ◽  
Clinical Diagnosis ◽  
Expression Profiling ◽  
Chronic Lung Allograft Dysfunction ◽  
Allograft Dysfunction

Microsatellites in immune-relevant regions and their associations with Maedi-Visna and ovine pulmonary adenocarcinoma viral diseases

2012 ◽  
Vol 145 (1-2) ◽  
pp. 438-446 ◽  
Author(s):  
A. Larruskain ◽  
E. Minguijón ◽  
I. Arostegui ◽  
B. Moreno ◽  
R.A. Juste ◽  
...  
Keyword(s):  
Pulmonary Adenocarcinoma ◽  
Viral Diseases ◽  
Ovine Pulmonary Adenocarcinoma

Overexpression of aldolase A and cytokeratin 19 in ovine pulmonary adenocarcinoma

2012 ◽  
Vol 15 (4) ◽  
pp. 703-709 ◽  
Author(s):  
A. Kycko ◽  
M. Reichert
Keyword(s):  
Epithelial Cells ◽  
Pulmonary Adenocarcinoma ◽  
Cytokeratin 19 ◽  
Immunohistochemical Examination ◽  
Neoplastic Cells ◽  
Ovine Pulmonary Adenocarcinoma ◽  
Immunohistochemical Studies ◽  
Aldolase A ◽  
Experimental Group ◽  
Dimensional Electrophoresis

Abstract Ovine pulmonary adenocarcinoma (OPA) is a transmissible lung cancer of sheep caused by jaagsiekte sheep retrovirus (JSRV). In the present study the protein profiles of five neoplastic and three non-neoplastic sheep lung tissues were examined for the identification of proteins overexpressed in ovine pulmonary adenocarcinoma. Lung sections of the experimental group of sheep were collected during necropsies for proteomic and immunohistochemical examination. Two dimensional electrophoresis (2DE) was performed using gel strips with immobilized pH gradient 3-10. As a result of 2DE gel analysis 14 spots characterized by over 2-fold higher expression in tumour proteomes were selected for mass spectrometry. In eleven spots more than one polypeptide was identified indicating overlapping of proteins in gels. In two spots demonstrating over 3-fold higher expression in OPA proteomes, single proteins: cytokerarin 19 (CK19) and aldolase A were identified. Immunohistochemical studies revealed that CK19 and aldolase A were expressed in the cytoplasm of epithelial cells of bronchioles in non-neoplastic lung sections, as well as epithelial cells of bronchioles and neoplastic cells in lung sections of OPA affected sheep. The results indicate that the overexpression of the two proteins reflects the presence of neoplastic cells in the lungs of OPA affected sheep.

Ovine pulmonary adenocarcinoma: the story to date

In Practice ◽  
2004 ◽  
Vol 26 (7) ◽  
pp. 387-392 ◽  
Author(s):  
Daniela Salvatori ◽  
Marcelo de las Heras ◽  
Mike Sharp
Keyword(s):  
Pulmonary Adenocarcinoma ◽  
Ovine Pulmonary Adenocarcinoma
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