Imaging and score-based quantification of ovine pulmonary adenocarcinoma using computed tomography as an additional tool in advanced clinical diagnosis

Until validated correlates of protection are identified, animal models remain the only way to test the efficacy of the new vaccines and drugs urgently needed to fight the global epidemic caused by infection with Mycobacterium tuberculosis. Non-human primates (NHP) offer the most relevant models of human tuberculosis (TB) and are central to the development process for new interventions. Efficacy evaluations are dependent on the capability of the test model to discriminate improved outcomes between treated groups after experimental exposure to M. tuberculosis and therefore the ability to measure TB-induced disease burden is central to the process. We have developed a score system that allows us to quantify the disease burden induced in macaques by infection with M. tuberculosis, based on the extent and features of disease visible on computed tomography (CT) images. The CT determined disease burden was then verified against that obtained using an established pathology-based approach. Trials of the system as a tool to measure disease burden have shown the approach capable of revealing differences between treatment groups in order to: (a) characterise outcome of infection and enable model refinement; (b) demonstrate the efficacy of drug treatment regimens by showing differences in outcome between test groups. Initial trials suggest that the imaging-based score system provides a valuable additional tool for the measurement of TB-induced disease burden that offers the opportunity to apply both refinement and reduction within studies.

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Tuboovarian Abscess Presenting as Sciatic Pain: Case Report

Neurosurgery ◽

10.1227/00006123-198707000-00024 ◽

1987 ◽

Vol 21 (1) ◽

pp. 100-103 ◽

Cited By ~ 8

Author(s):

David W. Andrews ◽

Neil B. Friedman ◽

Linda Heier ◽

Amelia Erickson ◽

Michael H. Lavyne

Keyword(s):

Computed Tomography ◽

Case Report ◽

Conservative Treatment ◽

Clinical Diagnosis ◽

Epidural Abscess ◽

Diagnosis And Treatment ◽

Low Grade ◽

First Report ◽

Sciatic Pain ◽

Tuboovarian Abscess

Abstract The authors report the successful conservative treatment of a tuboovarian abscess in a 25-year old woman who presented with low grade fever and severe right sciatic pain. Computed tomography and ultrasonography corroborated a clinical diagnosis of tuboovarian abscess after an initial diagnosis of epidural abscess had been made. This is the first report of a tuboovarian abscess presenting principally as sciatic pain. The diagnosis and treatment of this lesion are discussed. (Neurosurgery 21: 100-103, 1987)

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Detection of Jaagsiekte sheep retrovirus in apparently healthy sheep by real-time TaqMan PCR in comparison with histopathological findings

Journal of Veterinary Research ◽

10.1515/jvetres-2016-0002 ◽

2016 ◽

Vol 60 (1) ◽

pp. 7-12 ◽

Cited By ~ 2

Author(s):

Aliasghar Bahari ◽

Masoud Sabouri Ghannad ◽

Omid Dezfoulian ◽

Fereydon Rezazadeh ◽

Ali Sadeghi-Nasab

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Mediastinal Lymph Node ◽

Pulmonary Adenocarcinoma ◽

Ovine Pulmonary Adenocarcinoma ◽

Jaagsiekte Sheep Retrovirus ◽

Tissue Samples ◽

Blood Samples ◽

Proviral Dna ◽

Healthy Sheep

Abstract Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.

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A History of Ovine Pulmonary Adenocarcinoma (Jaagsiekte) and Experiments Leading to the Deduction of the JSRV Nucleotide Sequence

Current Topics in Microbiology and Immunology - Jaagsiekte Sheep Retrovirus and Lung Cancer ◽

10.1007/978-3-642-55638-8_1 ◽

2003 ◽

pp. 1-23 ◽

Cited By ~ 5

Author(s):

D. F. York ◽

G. Querat

Keyword(s):

Nucleotide Sequence ◽

Pulmonary Adenocarcinoma ◽

Ovine Pulmonary Adenocarcinoma ◽

History Of

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Carcinomatous encephalitis as clinical presentation of occult lung adenocarcinoma: case report

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x2007000500022 ◽

2007 ◽

Vol 65 (3b) ◽

pp. 841-844 ◽

Cited By ~ 5

Author(s):

Henrique Barbosa Ribeiro ◽

Tadeu Ferreira de Paiva Jr ◽

Gustavo Pignatari Rosas Mamprin ◽

Milton Luiz Gorzoni ◽

Antônio José da Rocha ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Computed Tomography ◽

Clinical Presentation ◽

Clinical Manifestations ◽

Mass Effect ◽

Pulmonary Adenocarcinoma ◽

Rare Entity ◽

Resonance Imaging ◽

Clinical Imaging ◽

Magnetic Resonance Imaging Mri

Carcinomatous encephalitis is a rare entity, originally described by Madow and Alpers in 1951, which is characterized by tumoral spreading perivascular, without mass effect. Clinical manifestations such as hemiparesis, seizures, ataxia, speech difficulties, cerebrospinal fluid findings as well as computed tomography are nonspecific. This leads the physician to pursue more frequent diseases that could explain those manifestations - toxic, metabolic, and/or infectious encephalopathy. A magnetic resonance imaging (MRI) with gadolinium, the method of choice, presumes the diagnosis. Previous reports of this unusual form of metastatic disease have described patients with prior diagnosis of pulmonary adenocarcinoma. We present the case of carcinomatous encephalitis in a 76-years-old woman as the primary manifestation of occult pulmonary adenocarcinoma with its clinical, imaging, and anatomopathological findings.

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