Evaluation of the survival of bone marrow-derived mononuclear cells and the growth factors produced upon intramedullary transplantation in rat models of acute spinal cord injury

2016 ◽  
Vol 107 ◽  
pp. 88-94 ◽  
Author(s):  
Kiyotaka Arai ◽  
Yasuji Harada ◽  
Hiroyuki Tomiyama ◽  
Masaki Michishita ◽  
Nobuo Kanno ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Growth Factors ◽  
Mononuclear Cells ◽  
Acute Spinal Cord Injury ◽  
Rat Models ◽  
Cord Injury

Bone marrow stromal cell transplantation for treatment of sub-acute spinal cord injury in the rat

2010 ◽  
Vol 1332 ◽  
pp. 32-47 ◽  
Author(s):  
Chizuka Ide ◽  
Yoshiyasu Nakai ◽  
Norihiko Nakano ◽  
Tae-Beom Seo ◽  
Yoshihiro Yamada ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Cell Transplantation ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Acute Spinal Cord Injury ◽  
Marrow Stromal Cell ◽  
Cord Injury

scholarly journals Two intrathecal transplants of bone marrow mononuclear cells produce motor improvement in an acute and severe model of spinal cord injury

2013 ◽  
Vol 12 (4) ◽  
pp. 274-277 ◽  
Author(s):  
Elisa Lettnin Kaminski ◽  
Asdrubal Falavigna ◽  
Gianina Terribele Venturin ◽  
Daniel Marinowic ◽  
Pamela Brambilla Bagatini ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Motor Function ◽  
Histological Analysis ◽  
Mononuclear Cells ◽  
Pcr Analysis ◽  
Bone Marrow Mononuclear Cells ◽  
Injury Site ◽  
Cord Injury

OBJECTIVE: We studied transplants of bone marrow mononuclear cells (BMMC) by lumbar puncture (LP) in a severe model of spinal cord injury (SCI) using clip compression. METHODS: BMMCs or saline solution were transplanted by LP 48 hours and 9 days post injury. Motor function was evaluated by BBB scale, histological analysis by Nissl technique and the verification of cell migration by PCR analysis. RESULTS: The BBB had significantly improved in rats treated with BMMCs by LP compared with controls (p<0.001). The histological analysis did not showed difference in the lesional area between the groups. The PCR analysis was able to found BMMCs in the injury site. CONCLUSIONS: two BMMC transplants by LP improved motor function in a severe model of SCI and BMMC was found in the injury site.

scholarly journals Effect of mesenchymal stem cell-incorporated hydroxyapatite-collagen scaffold on tissue repair in acute spinal cord injury, and the mechanism involved

2020 ◽  
Vol 19 (5) ◽  
pp. 1099-1103
Author(s):  
Zhipeng Yao ◽  
Wenge Liu ◽  
Chenyang Song
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Tissue Repair ◽  
Acute Spinal Cord Injury ◽  
Bone Marrow Mscs ◽  
Significant Difference ◽  
Cord Injury

Purpose: To study the effect of hydroxyapatite-collagen (HC) scaffold with mesenchymal stem cells (MSCs) on tissue repair in acute spinal cord  injury (SCI).Method: Adult female Sprague-Dawley rats weighing 200 - 230 g were randomly divided into two groups implanted either with bone marrow-MSCs (experimental group) or HC scaffold alone (control group). Spinal cord injury was induced using laminectomy, resulting in a 2.0-mm gap at T10 of the spinal cord. The gap was filled in both groups with 2-mm HC scaffold at day 10 of culture. Cellular development, viability, and proliferation inside the scaffold were determined. Angiogenesis was determined by measuring fibronectin (FN) immunofluorescence, von Willebrand factor (vWF), hypoxiainducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF).Results: HC scaffold strengthened MSCs. Bone marrow MSCs exhibited no statistically significant difference when compared with cells in culture at day 10 (47.03 ± 3.135 %, p > 0.05). Moreover, on days 5 and 10, FN deposition was higher in MSCs with scaffold than in scaffold-free MSCs. The expressions of FN, vWF, HIF-1α and VEGF were positively correlated, indicating that incorporation of HC scaffold into MSCs significantly improved tissue repair by improving angiogenesis via a differentiation process (p < 0.001).Conclusion: These findings suggest that HC scaffold with MSCs is a potential therapeutic procedure for spinal cord injury. Keywords: Mesenchymal stem cells, Hydroxyapatite-collagen, Spinal cord injury, HC scaffold

Neuroprotective Effect of Bone Marrow–Derived Mononuclear Cells Promoting Functional Recovery from Spinal Cord Injury

2007 ◽  
Vol 24 (6) ◽  
pp. 1026-1036 ◽  
Author(s):  
Tomoyuki Yoshihara ◽  
Masayoshi Ohta ◽  
Yutaka Itokazu ◽  
Naoya Matsumoto ◽  
Mari Dezawa ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Functional Recovery ◽  
Mononuclear Cells ◽  
Neuroprotective Effect ◽  
Cord Injury

Effects of Multiple Injection of Bone Marrow Mononuclear Cells on Spinal Cord Injury of Rats

2017 ◽  
Vol 34 (21) ◽  
pp. 3003-3011 ◽  
Author(s):  
Kenji Kanekiyo ◽  
Norihiko Nakano ◽  
Tamami Homma ◽  
Yoshihiro Yamada ◽  
Masahiro Tamachi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Mononuclear Cells ◽  
Bone Marrow Mononuclear Cells ◽  
Multiple Injection ◽  
Cord Injury

scholarly journals Bone Marrow Mesenchymal Stem Cells Transplantation on Acute Spinal Cord Injury

2020 ◽  
Vol 29 (2) ◽  
pp. 91-98
Author(s):  
Hua Song ◽  
Shiqi Suo ◽  
Chao Ning ◽  
Yang Zhang ◽  
Weidong Mu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Acute Spinal Cord Injury ◽  
Cord Injury ◽  
Marrow Mesenchymal Stem Cells ◽  
Stem Cells Transplantation

scholarly journals Index of CD34+ Cells and Mononuclear Cells in the Bone Marrow of Spinal Cord Injury Patients of Different Age Groups: A Comparative Analysis

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Vidyasagar Devaprasad Dedeepiya ◽  
Yegneswara Yellury Rao ◽  
Gosalakkal A. Jayakrishnan ◽  
Jutty K. B. C. Parthiban ◽  
Subramani Baskar ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
General Population ◽  
Mononuclear Cells ◽  
Age Groups ◽  
Reproductive Age ◽  
Cd34 Cells ◽  
Cord Injury ◽  
And Gender

Introduction. Recent evidence of safety and efficacy of Bone Marrow Mononuclear Cells (BMMNC) in spinal cord injury makes the Bone Marrow (BM) CD34+ percentage and the BMMNC count gain significance. The indices of BM that change with body mass index and aging in general population have been reported but seldom in Spinal Cord Injury (SCI) victims, whose parameters of relevance differ from general population. Herein, we report the indices of BMMNC in SCI victims. Materials and Methods. BMMNCs of 332 SCI patients were isolated under GMP protocols. Cell count by Trypan blue method and CD34+ cells by flow cytometry were documented and analysed across ages and gender. Results. The average BMMNC per ml in the age groups 0–20, 21–40, 41–60, and 61–80 years were 4.71, 4.03, 3.67, and 3.02 million and the CD34+ were 1.05%, 1.04%, 0.94%, and 0.93% respectively. The decline in CD34+ was sharp between 20–40 and 40–60 age groups. Females of reproductive age group had lesser CD34+. Conclusion. The BMMNC and CD34+ percentages decline with aging in SCI victims. Their lower values in females during reproductive age should be analysed for relevance to hormonal influence. This study offers reference values of BMMNC and CD34+ of SCI victims for successful clinical application.

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