Molecular Recognition Patterns Between Vitamin B12 and Human Serum AlbuminExplored Through STD–NMR and Spectroscopic Methods

2021 ◽  
pp. 119828
Author(s):  
Qiaomei Sun ◽  
Yuanming Zhai ◽  
Wenjing Wang ◽  
Na Gan ◽  
Shuangshuang Zhang ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Vitamin B12 ◽  
Human Serum ◽  
Spectroscopic Methods ◽  
Std Nmr

scholarly journals Co60 Vitamin B12 Binding Capacity of Human Leukocytes

Blood ◽  
1962 ◽  
Vol 19 (2) ◽  
pp. 229-235 ◽  
Author(s):  
LEO M. MEYER ◽  
EUGENE P. CRONKITE ◽  
INEZ F. MILLER ◽  
CLAIRE W MULZAC ◽  
IRVING JONES
Keyword(s):  
Vitamin B12 ◽  
Human Serum ◽  
Polycythemia Vera ◽  
Binding Capacity ◽  
Chronic Myelocytic Leukemia ◽  
Nonspecific Binding ◽  
Two Phase ◽  
Intact Cells ◽  
Myelocytic Leukemia ◽  
Neutrophilic Leukocytes

Abstract 1. Mature neutrophilic leukocytes show the highest Co60B12 binding capacity. 2. Less mature granulocytes, "blast" forms and eosinophils have little or no Co60B12 binding capacity. 3. Disintegrated mature leukocytes from chronic myelocytic leukemia and polycythemia vera show higher B12 binding capacity than intact cells. 4. Mature leukocytes from patients with chronic myelocytic leukemia and polycythemia vera show a two-phase B12 curve suggesting specific and nonspecific binding, similar to that observed in human serum. 5. Disintegration products from mature neutrophilic leukocytes probably contribute largely to increased B12 binding capacity of serum in chronic myelocytic leukemia and polycythemia vera.

Long-term (in)stability of folate and vitamin B12 in human serum

2012 ◽  
Vol 50 (10) ◽  
Author(s):  
Eugène H.J.M. Jansen ◽  
Piet K. Beekhof ◽  
Johannes W.J.M. Cremers ◽  
Erna Schenk
Keyword(s):  
Vitamin B12 ◽  
Human Serum ◽  
Folate And Vitamin B12

The Carriers of Native Vitamin B12 in Normal Human Serum

1977 ◽  
Vol 53 (5) ◽  
pp. 453-457 ◽  
Author(s):  
C. A. Hall
Keyword(s):  
Vitamin B12 ◽  
Human Serum ◽  
Isotope Dilution ◽  
Serum Vitamin ◽  
Total Serum ◽  
Plasma Vitamin ◽  
Isotope Dilution Assay ◽  
Dilution Assay ◽  
Normal Human ◽  
Transcobalamin Ii

1. After fractionation of the vitamin B12-binding proteins of ten normal sera the components containing transcobalamin II and R-type binders of vitamin B12 respectively were studied for endogenous vitamin B12 content by two distinct systems of vitamin B12 assay. 2. The measurements of total serum vitamin B12 by either bioassay with Euglena gracilis or a radioisotope dilution assay agreed closely. 3. The native vitamin B12 carried by transcobalamin II was higher as measured by bioassay than by isotope dilution assay. 4. The presence of the transcobalamin II fraction of human serum altered the key reaction between the binding reagent of the isotope dilution assay, so that this assay failed to measure vitamin B12 quantitatively. 5. Probably, the mean fraction of plasma vitamin B12 carried by transcobalamin II is in the range 20–30%.

Human serum albumin-specific recognition of the natural herbal extract of Stryphnodendron polyphyllum through STD NMR, hyphenations and docking simulation studies

RSC Advances ◽  
2015 ◽  
Vol 5 (30) ◽  
pp. 23431-23442 ◽  
Author(s):  
Sheraz A. K. Tanoli ◽  
Nazish U. Tanoli ◽  
Tatiani M. Bondancia ◽  
Saman Usmani ◽  
Zaheer Ul-Haq ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Docking Simulation ◽  
Herbal Extract ◽  
Rapid Screening ◽  
Simulation Studies ◽  
Specific Recognition ◽  
Screening And Identification ◽  
Std Nmr

Over the last two decades, new and more advanced strategies that help in the rapid screening and identification of new ligands for a specific macromolecule have become an important domain.

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