scholarly journals Determination of 4’-Hydroxyacetanilide in Leaves Extract of Aquilaria malaccencis by High Pressure Liquid Chromatograph

2015 ◽  
Vol 195 ◽  
pp. 2726-2733 ◽  
Author(s):  
Siti Khairun Nissa Afiffudden ◽  
Habsah Alwi ◽  
Ku Halim Ku Hamid
Keyword(s):  
High Pressure ◽  
Liquid Chromatograph ◽  
High Pressure Liquid Chromatograph

High Pressure Liquid Chromatographic Determination of Xanthomegnin in Corn

1978 ◽  
Vol 61 (3) ◽  
pp. 590-592
Author(s):  
Michael E Stack ◽  
Nathan L Brown ◽  
Robert M Eppley
Keyword(s):  
High Pressure ◽  
Silica Gel ◽  
Chromatographic Determination ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
High Pressure Liquid Chromatograph ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Layer Chromatography

Abstract A method is described for the detection and quantitative analysis of xanthomegnin in corn samples. Initial extraction with CHCI3 in the presence of 0.5M H3PO4 is followed by additional purification using silica gel column chromatography. A high pressure liquid chromatograph equipped with a microparticle silica gel column and a 405 nm absorbance detector is used for detection and quantitation of the xanthomegnin. The identity of xanthomegnin is confirmed by thin layer chromatography on silica gel plates developed with benzenemethanol- acetic acid (90-(-5+5). The recovery of xanthomegnin added to corn samples at levels of 0.75–9.6 mg/kg averaged 41% with a coefficient of variation of 25%.

Improved Method for High Pressure Liquid Chromatographic Determination of Chlorophacinone in Mouse Tissue

1982 ◽  
Vol 65 (6) ◽  
pp. 1299-1301
Author(s):  
Joseph B Addison
Keyword(s):  
High Pressure ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Mouse Tissue ◽  
Height Measurement ◽  
Liquid Chromatograph ◽  
Order Of Magnitude ◽  
High Pressure Liquid Chromatograph ◽  
Liquid Chromatographic

Abstract A simplified method is described for the determination of chlorophacinone, 2-[(p-chlorophenyl)- phenylacetyl]-l,3-indandione, in homogenized mice. Chlorophacinone is extracted with acetonitrile. After Florisil cleanup, the extract is injected into a high pressure liquid chromatograph for reverse phase chromatography on a polar Lichrosorb NH2 (10 μm) column, with a mobile phase of acetonitrile-water (80 + 20). An injection containing 70 ng chlorophacinone produces 1/2 scale peaks at 254 nm with a full scale absorbance of 0.1 unit, an order of magnitude improvement over the sensitivity reported earlier with a 280 nm detector. Six homogenized mice samples and six spiked homogenized mice samples were quantitatively analyzed for trace levels of chlorophacinone by this method. Recoveries from spiked samples, as determined by peak height measurement, were >95%. Mean retention time for the chlorophacinone peaks in all samples was 6.05 ± 0.05 min. Chlorophacinone levels determined in homogenized whole mouse samples ranged from 0 to 63 ppm.

High Pressure Liquid Chromatographic Determination of Chlorophacinone in Formulations

1979 ◽  
Vol 62 (5) ◽  
pp. 1001-1003
Author(s):  
Ralph G Grant ◽  
Richard K Pike
Keyword(s):  
High Pressure ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Whole Grain ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
High Pressure Liquid Chromatograph ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple and rapid method is described determining for 2- ((p-chlorophenyl) phenylacetyl-1,3- indandione (chlorophacinone) in rodenticides formulated as tracking powders and whole grain and crushed grain baits. The bait is extracted with methanol containing an internal standard, and the extract is injected into a high pressure liquid chromatograph. The sample is analyzed by reverse phase chromatography on octadecyl (C18) bonded to glass beads with a mobile phase of methanolwater (35+65) plus 0.75% NH4OH. A 5 μL injection containing 240 ng benzophenone internal standard and 77 ng chlorophacinone produces half scale peaks at 280 nm with a full scale absorbance of 0.01 absorbance unit. Two formulations and a spiked sample were analyzed by the method. Recovery as determined by peak area was >97%.

High Pressure Liquid Chromatographic Determination of N-3-Pyridylmethyl-N′-p-nitrophenylurea in Rodenticides

1977 ◽  
Vol 60 (6) ◽  
pp. 1375-1378
Author(s):  
Carl W Sims ◽  
Richard K Gard
Keyword(s):  
High Pressure ◽  
Methylene Chloride ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Liquid Chromatograph ◽  
Two Samples ◽  
High Pressure Liquid Chromatograph ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple and rapid method is described for determining N′-3-pyridylmethyl-N′-p-nitrophenylurea (RH-787) in Vacor Ratkiller rodenticide. The bait is extracted with acetone, the extract is evaporated to dryness, an internal standard is added, and the solution is injected into a high pressure liquid chromatograph. The sample is analyzed by normal phase chromatography on a Partisil 10 column with a mobile phase of methanol-methylene chloride (10+90). An 8 μl injection containing 4 μg pyridine internal standard and 8 μg RH-787 produces half-scale peaks at 254 nm with a full scale absorbance of 0.5 absorbance unit. In an alternative procedure, the active ingredient is extracted from the bait with acetone and titrated to a methyl red end point with 0.1N perchloric acid dissolved in acetic acid. Two samples of Vacor Ratkiller and a spiked blank were analyzed by both procedures.

Switching Valve Designed For High Pressure Liquid Chromatographic Fraction Collection

1979 ◽  
Vol 62 (6) ◽  
pp. 1355-1357
Author(s):  
William O Landen
Keyword(s):  
High Pressure ◽  
Oil Removal ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Switching Valve ◽  
Gel Permeation ◽  
In Series ◽  
High Pressure Liquid Chromatograph ◽  
Pass Through ◽  
Liquid Chromatographic

Abstract The use of a collection valve specifically designed for high pressure liquid chromatography is described. Application of the valve to high pressure gel permeation chromatographic (GPC) separation of oil from the vitamin A active fraction of margarine resulted in efficient oil removal after one pass through 2 μStyragel (100A) columns connected in series. Using the normal collection mode of the high pressure liquid chromatograph, 2 passes through the GPC columns were required to adequately resolve the fractions.

Sample Preparation of Carbadox, Furazolidone, Nitrofurazone, and Ethopabate in Medicated Feeds for High Pressure Liquid Chromatography

1980 ◽  
Vol 63 (5) ◽  
pp. 981-984
Author(s):  
Virginia A Thorpe
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Room Temperature ◽  
Quantitative Measurement ◽  
Liquid Chromatograph ◽  
High Pressure Liquid Chromatograph ◽  
Medicated Feeds ◽  
Colorimetric Methods ◽  
Good Agreement

Abstract Medicated feeds (pelleted or mash) containing guarantees of carbadox, furazolidone, nitrofurazone, and ethopabate are pretreated with water, extracted with 95% dimethylformamide overnight at room temperature, cleaned up on a column of alumina, and injected into a high pressure liquid chromatograph for quantitative measurement. Carbadox, nitrofurazone, and furazolidone can be separated; chromatograms show excellent baseline resolution, and results are in good agreement with colorimetric methods. The same extraction and cleanup can be used to improve colorimetric methods for furazolidone and nitrofurazone.

High Pressure Liquid Chromatographic Determination of Nitrofurazone in Milk

1979 ◽  
Vol 62 (1) ◽  
pp. 19-22 ◽  
Author(s):  
Arnost B Vilim ◽  
Agnes I Macintosh
Keyword(s):  
High Pressure ◽  
Screening Method ◽  
Chromatographic Determination ◽  
Organic Layer ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Milk Serum ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A rapid high pressure liquid chromatographic (HPLC) screening method for the quantitative determination of nitrofurazone in milk has been developed. The drug is extracted with ethyl acetate from a 2.0 ml milk serum sample, the organic layer is evaporated to dryness, and the residue is dissolved in the mobile phase and injected into the liquid chromatograph. A reverse phase μBondapak C18 column is used with monitoring at 365 nm. The detection limit is 5 ppb and recoveries are 57—67%. Mass spectroscopic confirmation of the HPLC nitrofuran peak is described.

Sign in / Sign up

Export Citation Format

Share Document