Prevalence of antibiotic-resistant fecal bacteria in a river impacted by both an antibiotic production plant and urban treated discharges

2014 ◽  
Vol 488-489 ◽  
pp. 220-227 ◽  
Author(s):  
Ricardo Sidrach-Cardona ◽  
María Hijosa-Valsero ◽  
Elisabet Marti ◽  
José Luis Balcázar ◽  
Eloy Becares
Keyword(s):  
Antibiotic Production ◽  
Production Plant ◽  
Fecal Bacteria ◽  
Antibiotic Resistant

scholarly journals Structural and functional basis of transcriptional regulation by TetR family protein CprB from S. coelicolor A3(2)

2014 ◽  
Vol 42 (15) ◽  
pp. 10122-10133 ◽  
Author(s):  
Hussain Bhukya ◽  
Ruchika Bhujbalrao ◽  
Aruna Bitra ◽  
Ruchi Anand
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Dna Sequences ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Antibiotic Resistant ◽  
Transporter Protein ◽  
Dimeric Interface ◽  
Transcriptional Regulatory ◽  
Family Protein

AbstractAntibiotic production and resistance pathways in Streptomyces are dictated by the interplay of transcriptional regulatory proteins that trigger downstream responses via binding to small diffusible molecules. To decipher the mode of DNA binding and the associated allosteric mechanism in the sub-class of transcription factors that are induced by γ-butyrolactones, we present the crystal structure of CprB in complex with the consensus DNA element to a resolution of 3.25 Å. Binding of the DNA results in the restructuring of the dimeric interface of CprB, inducing a pendulum-like motion of the helix-turn-helix motif that inserts into the major groove. The crystal structure revealed that, CprB is bound to DNA as a dimer of dimers with the mode of binding being analogous to the broad spectrum multidrug transporter protein QacR from the antibiotic resistant strain Staphylococcus aureus. It was demonstrated that the CprB displays a cooperative mode of DNA binding, following a clamp and click model. Experiments performed on a subset of DNA sequences from Streptomyces coelicolor A3(2) suggest that CprB is most likely a pleiotropic regulator. Apart from serving as an autoregulator, it is potentially a part of a network of proteins that modulates the γ-butyrolactone synthesis and antibiotic regulation pathways in S. coelicolor A3(2).

scholarly journals Carriage of Antibiotic‐Resistant Fecal Bacteria in Nepal

2002 ◽  
Vol 185 (10) ◽  
pp. 1542-1542
Author(s):  
Prajwol P. Joshi
Keyword(s):  
Fecal Bacteria ◽  
Antibiotic Resistant

scholarly journals Isolation of High Antibiotic Resistant Fecal Bacteria Indicators, Salmonella and VibrioSpecies from Raw Abattoirs Sewage in Peri-Urban Locations of Nairobi, Kenya

2013 ◽  
Vol 3 (5) ◽  
pp. 172-178 ◽  
Author(s):  
Rosemary Atieno Nyamboya ◽  
◽  
Paul Owuor Okemo ◽  
Omwoyo Ombori ◽  
◽  
...  
Keyword(s):  
Fecal Bacteria ◽  
Antibiotic Resistant

scholarly journals Industrial Composting of Sewage Sludge: Study of the Bacteriome, Sanitation, and Antibiotic-Resistant Strains

2021 ◽  
Vol 12 ◽  
Author(s):  
Juan A. López-González ◽  
María J. Estrella-González ◽  
Rosario Lerma-Moliz ◽  
Macarena M. Jurado ◽  
Francisca Suárez-Estrella ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Bacterial Population ◽  
Raw Materials ◽  
Fecal Bacteria ◽  
Huge Amount ◽  
Antibiotic Resistant ◽  
Rrna Phylogeny ◽  
Resistant Strains ◽  
Composting Facilities ◽  
Industrial Composting

Wastewater treatment generates a huge amount of sewage sludge, which is a source of environmental pollution. Among the alternatives for the management of this waste, industrial composting stands out as one of the most relevant. The objective of this study was to analyze the bacterial population linked to this process and to determine its effectiveness for the reduction, and even elimination, of microorganisms and pathogens present in these organic wastes. For this purpose, the bacteriome and the fecal bacteria contamination of samples from different sewage sludge industrial composting facilities were evaluated. In addition, fecal bacteria indicators and pathogens, such as Salmonella, were isolated from samples collected at key stages of the process and characterized for antibiotic resistance to macrolide, β-lactam, quinolone, and aminoglycoside families. 16S rRNA phylogeny data revealed that the process clearly evolved toward a prevalence of Firmicutes and Actinobacteria phyla, removing the fecal load. Moreover, antibiotic-resistant microorganisms present in the raw materials were reduced, since these were isolated only in the bio-oxidative phase. Therefore, industrial composting of sewage sludge results in a bio-safe final product suitable for use in a variety of applications.

scholarly journals Carriage of Antibiotic‐Resistant Fecal Bacteria in Nepal Reflects Proximity to Kathmandu

2001 ◽  
Vol 184 (9) ◽  
pp. 1163-1169 ◽  
Author(s):  
Judd L. Walson ◽  
Bonnie Marshall ◽  
B. M. Pokhrel ◽  
K. K. Kafle ◽  
Stuart B. Levy
Keyword(s):  
Fecal Bacteria ◽  
Antibiotic Resistant

scholarly journals Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation

2020 ◽  
Vol 23 (3) ◽  
pp. 89-95
Author(s):  
Citra Hardiyanti ◽  
Khairullinas Khairullinas ◽  
Jeky Sasemar Lumban ◽  
Titania Tjandrawati Nugroho ◽  
Yuana Nurulita
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Antibiotic Production ◽  
Multidrug Resistant ◽  
Positive Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Penicillium Sp ◽  
Biotic Elicitor

An antibiotic-resistant and multidrug-resistant (MDR) issue open the role of researchers to continue to search for natural potential as a source of new antimicrobials. One of the potential fungi isolates that can produce antimicrobial active compounds from Indonesian tropical peat soils is Penicillium sp. LBKURCC34. In this study, the production of antimicrobial compounds from local isolates was carried out by batch fermentation method in liquid media with the addition of biotic elicitors to increase the extraction activity and yield. This study aims to optimize the results based on the time the elicitor is added. Staphylococcus aureus was used as a biotic elicitor, which was added on days 2, 3, and 4 in the production of antibiotics by fermentation incubation of 6-14 days. The antibiotic production media was extracted with ethyl acetate and evaporated. The antimicrobial test was carried out by the disk diffusion method against pathogenic bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Staphylococcus epidermidis using three crude extract contents (19; 38; and 57 µg/disc). Amoxicillin® was used as a positive control (10 µL/disc). The results showed that the addition of S. aureus biotic elicitor extended the log phase growth of the fungus Penicillium sp. LBKURCC34. The optimum condition of production was obtained by adding initiator treatment on the 3rd day for 14 days incubation with the highest yield and could inhibit the growth of all pathogenic microbes.

Use of digital microscopy in process control during the manufacture of rubber-modified thermoplastics

1996 ◽  
Vol 54 ◽  
pp. 616-617
Author(s):  
M. T. Dineen
Keyword(s):  
Ccd Camera ◽  
Operating Costs ◽  
Hard Copy ◽  
Production Plant ◽  
Digital Microscopy ◽  
Nih Image ◽  
Transmission Electron ◽  
Conventional Film ◽  
Product Loss ◽  
Image Collection

The production of rubber modified thermoplastics can exceed rates of 30,000 pounds per hour. If a production plant needs to equilibrate or has an upset, that means operating costs and lost revenue. Results of transmission electron microscopy (TEM) can be used for process adjustments to minimize product loss. Conventional TEM, however, is not a rapid turnaround technique. The TEM process was examined, and it was determined that 50% of the time it took to complete a polymer sample was related to film processing, even when using automated equipment. By replacing the conventional film portion of the process with a commercially available system to digitally acquire the TEM image, a production plant can have the same TEM image in the control room within 1.5 hours of sampling.A Hitachi H-600 TEM Operated at 100 kV with a tungsten filament was retrofitted with a SEMICAPS™ image collection and processing workstation and a KODAK MEGAPLUS™ charged coupled device (CCD) camera (Fig. 1). Media Cybernetics Image-Pro Plus software was included, and connections to a Phaser II SDX printer and the network were made. Network printers and other PC and Mac software (e.g. NIH Image) were available. By using digital acquisition and processing, the time it takes to produce a hard copy of a digital image is greatly reduced compared to the time it takes to process film.

THE BACTERIAL MULTIPLE ANTIBIOTIC RESISTANT (MAR) PHENOTYPE LEADS TO INCREASED TOLERANCE TO TEA TREE OIL

Pathology ◽  
2001 ◽  
Vol 33 (2) ◽  
pp. 211-215 ◽  
Author(s):  
John E. Gustafson ◽  
Sean D. Cox ◽  
Yoon C. Liew ◽  
S. Grant Wyllie ◽  
John R. Warmington
Keyword(s):  
Tea Tree Oil ◽  
Antibiotic Resistant ◽  
Tea Tree
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