Development of a phage chemiluminescent enzyme immunoassay with high sensitivity for the determination of imidaclothiz in agricultural and environmental samples

Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC50) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%–108.3% (ic-ELISA) and 80.1%–98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy.

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Electrodeposition of Pd-Pt Nanocomposites on Porous GaN for Electrochemical Nitrite Sensing

Sensors ◽

10.3390/s19030606 ◽

2019 ◽

Vol 19 (3) ◽

pp. 606 ◽

Cited By ~ 7

Author(s):

Rui Xi ◽

Shao-Hui Zhang ◽

Long Zhang ◽

Chao Wang ◽

Lu-Jia Wang ◽

...

Keyword(s):

Environmental Samples ◽

Food Industry ◽

Biological Process ◽

High Sensitivity ◽

Good Reproducibility ◽

Practical Application ◽

Long Term Stability ◽

Nitrite Content

In recent years, nitrite pollution has become a subject of great concern for human lives, involving a number of fields, such as environment, food industry and biological process. However, the effective detection of nitrite is an instant demand as well as an unprecedented challenge. Here, a novel nitrite sensor was fabricated by electrochemical deposition of palladium and platinum (Pd-Pt) nanocomposites on porous gallium nitride (PGaN). The obtained Pd-Pt/PGaN sensor provides abundant electrocatalytic sites, endowing it with excellent performances for nitrite detection. The sensor also shows a low detection limit of 0.95 µM, superior linear ampere response and high sensitivity (150 µA/mM for 1 to 300 µM and 73 µA/mM for 300 to 3000 µM) for nitrite. In addition, the Pd-Pt/PGaN sensor was applied and evaluated in the determination of nitrite from the real environmental samples. The experimental results demonstrate that the sensor has good reproducibility and long-term stability. It provides a practical way for rapidly and effectively monitoring nitrite content in the practical application.

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Determination of progesterone receptor by chemiluminescent enzyme immunoassay

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/bf02829465 ◽

2003 ◽

Vol 23 (1) ◽

pp. 60-61

Author(s):

Yin Tiejun ◽

Gu Meijiao ◽

Zhou Yikai ◽

Zheng Wen ◽

Hu Wei ◽

...

Keyword(s):

Progesterone Receptor ◽

Enzyme Immunoassay ◽

Chemiluminescent Enzyme Immunoassay

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Determination of Free Triiodothyronine by Chemiluminescent Enzyme Immunoassay

Chinese Journal of Analytical Chemistry ◽

10.1016/s1872-2040(08)60035-3 ◽

2008 ◽

Vol 36 (5) ◽

pp. 609-614 ◽

Cited By ~ 6

Author(s):

Zhen LIN ◽

Xu WANG ◽

Shi-Qi REN ◽

Guo-Nan CHEN ◽

Zhen-Jia LI ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Free Triiodothyronine ◽

Chemiluminescent Enzyme Immunoassay

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Development of sensitive direct chemiluminescent enzyme immunoassay for the determination of dihydroartemisinin in plasma

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-015-8951-4 ◽

2015 ◽

Vol 407 (25) ◽

pp. 7823-7830 ◽

Cited By ~ 10

Author(s):

Laura Zehnacker ◽

Marie-Claire Nevers ◽

Véronique Sinou ◽

Dominique Parzy ◽

Christophe Créminon ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Chemiluminescent Enzyme Immunoassay

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Quantitative analysis of chloramphenicol residues in shrimp muscle tissues by Chemiluminescent enzyme immunoassay

Czech Journal of Food Sciences ◽

10.17221/3399-cjfs ◽

2011 ◽

Vol 23 (No. 6) ◽

pp. 251-256

Author(s):

X. Chuanlai ◽

P. Cifang ◽

H. Kai ◽

J. Zhengyu ◽

W. Wukang

Keyword(s):

Enzyme Immunoassay ◽

Limit Of Detection ◽

Tween 20 ◽

Detection Range ◽

Muscle Tissues ◽

Physico Chemical ◽

Interassay Coefficient ◽

Chemiluminescent Enzyme Immunoassay ◽

Better Than

A competitive indirect chemiluminescent enzyme immunoassay (ic-CLEIA) has been developed for the determination of chloramphenicol (CAP) residues in shrimp. After the optimisation of four physico-chemical parameters, i.e. incubation time, concentration of Tween-20, concentration of PBS and its pH, the method developed gave a limit of detection of 0.01 ng/ml and a detection range from 0.03 ng/ml to 23.7 ng/ml, with an ED<sub>50</sub> of 0.47 ng/ml. The developed method has been validated on spiked shrimp samples in terms of precision (intra- and interassay coefficient variations of less than 10% and 15%, respectively), and of accuracy (mean recovery from 95% to 123%). All these parameters being better than those of the ELISA method which is widely used to detect chloramphenicol, it may be suggested that the CLEIA method can be used to detect aquatic samples instead of ELISA.  

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