Vibrio fischeri bioluminescence inhibition assay for ecotoxicity assessment: A review

2018 ◽  
Vol 626 ◽  
pp. 1295-1309 ◽  
Author(s):  
Mazhar Abbas ◽  
Muhammad Adil ◽  
Syed Ehtisham-ul-Haque ◽  
Bushra Munir ◽  
Muhammad Yameen ◽  
...  
Keyword(s):  
Vibrio Fischeri ◽  
Inhibition Assay ◽  
Bioluminescence Inhibition ◽  
Bioluminescence Inhibition Assay

General baseline toxicity QSAR for nonpolar, polar and ionisable chemicals and their mixtures in the bioluminescence inhibition assay with Aliivibrio fischeri

2017 ◽  
Vol 19 (3) ◽  
pp. 414-428 ◽  
Author(s):  
Beate I. Escher ◽  
Andreas Baumer ◽  
Kai Bittermann ◽  
Luise Henneberger ◽  
Maria König ◽  
...  
Keyword(s):  
Distribution Ratio ◽  
Water Distribution ◽  
Qsar Model ◽  
Inhibition Assay ◽  
Microtox Assay ◽  
Bioluminescence Inhibition ◽  
Aliivibrio Fischeri ◽  
Acids And Bases ◽  
Bioluminescence Inhibition Assay

A general QSAR model for the Microtox assay with the ionisation-corrected liposome–water distribution ratio is applicable to diverse chemicals including acids and bases.

scholarly journals Bioluminescence Inhibition Assay for the Detection of Hydroxylated Polychlorinated Biphenyls

2012 ◽  
Vol 84 (18) ◽  
pp. 7648-7655 ◽  
Author(s):  
Krystal Teasley Hamorsky ◽  
C. Mark Ensor ◽  
Emre Dikici ◽  
Patrizia Pasini ◽  
Leonidas Bachas ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Inhibition Assay ◽  
Hydroxylated Polychlorinated Biphenyls ◽  
Bioluminescence Inhibition ◽  
Bioluminescence Inhibition Assay

scholarly journals Comparison of organics and heavy metals acute toxicities to Vibrio fischeri

2016 ◽  
Vol 81 (6) ◽  
pp. 697-705 ◽  
Author(s):  
Xuepeng Yang ◽  
Yan Ji ◽  
Fangfang Wang ◽  
Jia Xu ◽  
Xiangzhen Liu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cell Death ◽  
Benzoic Acid ◽  
Death Rate ◽  
Vibrio Fischeri ◽  
Reaction Times ◽  
Inhibition Rate ◽  
Cell Death Rate ◽  
Bioluminescence Inhibition ◽  
The Difference

Vibrio fischeri bioluminescence inhibition has been widely used to test acute toxicities of metals and organics contaminants. However, the differences of metals and organics acute toxicities to V. fischeri have not been compared. Here, four heavy metals (Zn2+, Cu2+, Cd2+, Cr6+) and five organics (phenol, benzoic acid, p-hydroxy benzoic acid, nitro-benzene and benzene) acute toxicities to V. fischeri were investigated. Heavy metals toxicities to V. fischeri were increased along with the reaction time, while the organics toxicities kept the same level in different reaction times. In order to explain the difference, the relative cell death rate of V. fischeri was detected. In metals toxicities tests, the bioluminescence inhibition rate of V. fischeri was found to be significantly higher than the relative cell death rate (P<0.05), while for the organics toxicities tests, the cell death rate was similar to the bioluminescence inhibition rate. These results indicated that organics acute toxicities to V. fischeri could reflect the death of cell, but metals acute toxicities to V. fischeri may not lead to the death of cell, just represent the bioluminescence inhibition.

scholarly journals Comparative assessment of ecotoxicity of urban aerosol

2012 ◽  
Vol 12 (4) ◽  
pp. 8533-8546 ◽  
Author(s):  
B. Turóczi ◽  
A. Hoffer ◽  
Á. Tóth ◽  
N. Kováts ◽  
A. Ács ◽  
...  
Keyword(s):  
Air Pollution ◽  
Health Effects ◽  
Vibrio Fischeri ◽  
Urban Aerosol ◽  
Acute Effects ◽  
Direct Emission ◽  
Aerosol Pollution ◽  
Polycyclic Aromatic ◽  
Bioluminescence Inhibition ◽  
Pollution Episodes

Abstract. In addition to its mass concentration, the health effects of urban particulate matter may depend on its particle size distribution and chemical composition. Yet air pollution regulations rely on exclusively bulk PM10 concentration measurements, without regard to their potentially different health effects under different conditions. Aerosols from various sources are well known to contain a plethora of toxic, carcinogenic, mutagenic or teratogenic constituents such as heavy metals and polycyclic aromatic hydrocarbons. In spite of the fact that tremendous efforts have been put to establish links between aerosol pollution and human health or mortality, the potential acute effects of PM2.5/PM10 have never been assessed for lack of adequate methodology. Here we present the application of a simple and sensitive method for the direct assessment of the overall ecotoxicity of various PM2.5/PM10 samples collected on filters. The method is based on the Vibrio fischeri bioluminescence inhibition bioassay that has been standardized for solid samples, representing a relevant biological exposure route. Direct emission samples proved to be significantly more ecotoxic than photochemically processed aerosol, thus marked differences were observed between the ecotoxicities of urban PM10 in summer and winter. The previously overlooked acute effects of urban PM10 may add to the established effects of gaseous primary pollutants aggravating health problems during severe air pollution episodes.

Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri

2008 ◽  
Vol 1 (2) ◽  
pp. 189-193 ◽  
Author(s):  
S. Sarter ◽  
I. Metayer ◽  
N. Zakhia
Keyword(s):  
Aflatoxin B1 ◽  
Light Emission ◽  
Vibrio Fischeri ◽  
Exponential Phase ◽  
Lag Phase ◽  
Bioluminescence Inhibition ◽  
Aflatoxin B

The effects of aflatoxin B1 and deoxynivalenol on the luminescence of Vibrio fischeri were investigated to determine the conditions of using the bioluminescence as an indirect means for mycotoxin detection. The culture of Vibrio fischeri showed that bioluminescence reached a peak after 12 hours of incubation at 25 °C and then decreased drastically. During the lag phase which lasted 6 hours, light emission decreased drastically for both the mycotoxin assays – aflatoxin B1 10 µg/ml and deoxynivalenol 20 µg/ml – and the corresponding controls. Distinct bioluminescence inhibition appeared after this period of minimal bioluminescence of the controls and started with the exponential phase of growth. The percentage of bioluminescence inhibition for both mycotoxins was determined after 3.5, 10, 15 and 25 hours of incubation. The bioluminescence of Vibrio fischeri was inhibited with aflatoxin B1 and enhanced with deoxynivalenol. Both effects were delayed and required a long-term incubation over 10 hours, which may help to investigate bioassays for mycotoxin detection.

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