European ring exercise on water toxicity using different bioluminescence inhibition tests based on Vibrio fischeri, in support to the implementation of the water framework directive

Talanta ◽  
2006 ◽  
Vol 69 (2) ◽  
pp. 323-333 ◽  
Author(s):  
M FARRE ◽  
E MARTINEZ ◽  
M HERNANDO ◽  
A FERNANDEZALBA ◽  
J FRITZ ◽  
...  
Keyword(s):  
Water Framework Directive ◽  
Vibrio Fischeri ◽  
Water Toxicity ◽  
Bioluminescence Inhibition

scholarly journals Comparison of organics and heavy metals acute toxicities to Vibrio fischeri

2016 ◽  
Vol 81 (6) ◽  
pp. 697-705 ◽  
Author(s):  
Xuepeng Yang ◽  
Yan Ji ◽  
Fangfang Wang ◽  
Jia Xu ◽  
Xiangzhen Liu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cell Death ◽  
Benzoic Acid ◽  
Death Rate ◽  
Vibrio Fischeri ◽  
Reaction Times ◽  
Inhibition Rate ◽  
Cell Death Rate ◽  
Bioluminescence Inhibition ◽  
The Difference

Vibrio fischeri bioluminescence inhibition has been widely used to test acute toxicities of metals and organics contaminants. However, the differences of metals and organics acute toxicities to V. fischeri have not been compared. Here, four heavy metals (Zn2+, Cu2+, Cd2+, Cr6+) and five organics (phenol, benzoic acid, p-hydroxy benzoic acid, nitro-benzene and benzene) acute toxicities to V. fischeri were investigated. Heavy metals toxicities to V. fischeri were increased along with the reaction time, while the organics toxicities kept the same level in different reaction times. In order to explain the difference, the relative cell death rate of V. fischeri was detected. In metals toxicities tests, the bioluminescence inhibition rate of V. fischeri was found to be significantly higher than the relative cell death rate (P<0.05), while for the organics toxicities tests, the cell death rate was similar to the bioluminescence inhibition rate. These results indicated that organics acute toxicities to V. fischeri could reflect the death of cell, but metals acute toxicities to V. fischeri may not lead to the death of cell, just represent the bioluminescence inhibition.

scholarly journals Water toxicity monitoring using Vibrio fischeri: a method free of interferences from colour and turbidity

2004 ◽  
Vol 6 (2) ◽  
pp. 97 ◽  
Author(s):  
Elsa Correia Faria ◽  
Bernard J. Treves Brown ◽  
Richard D. Snook
Keyword(s):  
Vibrio Fischeri ◽  
Water Toxicity ◽  
Toxicity Monitoring

scholarly journals Comparative assessment of ecotoxicity of urban aerosol

2012 ◽  
Vol 12 (4) ◽  
pp. 8533-8546 ◽  
Author(s):  
B. Turóczi ◽  
A. Hoffer ◽  
Á. Tóth ◽  
N. Kováts ◽  
A. Ács ◽  
...  
Keyword(s):  
Air Pollution ◽  
Health Effects ◽  
Vibrio Fischeri ◽  
Urban Aerosol ◽  
Acute Effects ◽  
Direct Emission ◽  
Aerosol Pollution ◽  
Polycyclic Aromatic ◽  
Bioluminescence Inhibition ◽  
Pollution Episodes

Abstract. In addition to its mass concentration, the health effects of urban particulate matter may depend on its particle size distribution and chemical composition. Yet air pollution regulations rely on exclusively bulk PM10 concentration measurements, without regard to their potentially different health effects under different conditions. Aerosols from various sources are well known to contain a plethora of toxic, carcinogenic, mutagenic or teratogenic constituents such as heavy metals and polycyclic aromatic hydrocarbons. In spite of the fact that tremendous efforts have been put to establish links between aerosol pollution and human health or mortality, the potential acute effects of PM2.5/PM10 have never been assessed for lack of adequate methodology. Here we present the application of a simple and sensitive method for the direct assessment of the overall ecotoxicity of various PM2.5/PM10 samples collected on filters. The method is based on the Vibrio fischeri bioluminescence inhibition bioassay that has been standardized for solid samples, representing a relevant biological exposure route. Direct emission samples proved to be significantly more ecotoxic than photochemically processed aerosol, thus marked differences were observed between the ecotoxicities of urban PM10 in summer and winter. The previously overlooked acute effects of urban PM10 may add to the established effects of gaseous primary pollutants aggravating health problems during severe air pollution episodes.

Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri

2008 ◽  
Vol 1 (2) ◽  
pp. 189-193 ◽  
Author(s):  
S. Sarter ◽  
I. Metayer ◽  
N. Zakhia
Keyword(s):  
Aflatoxin B1 ◽  
Light Emission ◽  
Vibrio Fischeri ◽  
Exponential Phase ◽  
Lag Phase ◽  
Bioluminescence Inhibition ◽  
Aflatoxin B

The effects of aflatoxin B1 and deoxynivalenol on the luminescence of Vibrio fischeri were investigated to determine the conditions of using the bioluminescence as an indirect means for mycotoxin detection. The culture of Vibrio fischeri showed that bioluminescence reached a peak after 12 hours of incubation at 25 °C and then decreased drastically. During the lag phase which lasted 6 hours, light emission decreased drastically for both the mycotoxin assays – aflatoxin B1 10 µg/ml and deoxynivalenol 20 µg/ml – and the corresponding controls. Distinct bioluminescence inhibition appeared after this period of minimal bioluminescence of the controls and started with the exponential phase of growth. The percentage of bioluminescence inhibition for both mycotoxins was determined after 3.5, 10, 15 and 25 hours of incubation. The bioluminescence of Vibrio fischeri was inhibited with aflatoxin B1 and enhanced with deoxynivalenol. Both effects were delayed and required a long-term incubation over 10 hours, which may help to investigate bioassays for mycotoxin detection.

scholarly journals Preliminary ecotoxicity assessment of selected flavour and fragrance compounds using Microtox assay

2019 ◽  
Vol 100 ◽  
pp. 00036
Author(s):  
Wojciech Kołodyński ◽  
Katarzyna Piekarska ◽  
Daniel Strub
Keyword(s):  
Marine Bacteria ◽  
Vibrio Fischeri ◽  
Inorganic Compounds ◽  
Chemical Compounds ◽  
Potential Threat ◽  
Chemical Substances ◽  
Microtox Assay ◽  
Bioluminescence Inhibition ◽  
The Many ◽  
Fragrance Compounds

The bioluminescence inhibition bioassay using marine bacteria Vibrio fischeri is widely used as a tool to assess the toxicity of chemical compounds, because of the many benefits comprising cost and time effectiveness, rapidness, sensitivity, and ease of operation. In addition, the test is used for various types of organic and inorganic compounds. Due to the insolubility of organic compounds in water, a suitable solubilizer should be applied. The ecotoxicity of the solvent should be negligible in relation to marine bacteria. On account of superior human activities the synthesis of new chemical substances is inextricably linked to the emergence of chemical compounds that are a potential threat on environment. A Microtox system utilizing the 81.9% Basic Test with 14 dilutions was applied to pre-evaluate the ecotoxicity of β-cyclocitral oxime, citronellal oxime and perillaldehyde oxime. Substances solutions with an initial concentration of 0.036% in DMSO were prepared. The preliminary results show that the studied scent compounds are characterized by quite high toxicity. In order to confirm the received ecotoxicity values, it is necessary to carry out additional surveys using another range of concentrations and biotests on organisms at each trophic level.

scholarly journals Toxicity of Some Pesticides, Heavy Metals and Their Mixtures to Vibrio fischeri Bacteria and Daphnia magna: Comparative Study

2015 ◽  
Vol 6 (2) ◽  
pp. 221 ◽  
Author(s):  
Sameeh A. Mansour ◽  
Alia A. Abdel-Hamid ◽  
Azza W. Ibrahim ◽  
Neveen H. Mahmoud ◽  
Walaa A. Moselhy
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Daphnia Magna ◽  
Exposure Time ◽  
Vibrio Fischeri ◽  
The Other ◽  
Metal Mixtures ◽  
Pesticide Mixtures ◽  
Bioluminescence Inhibition ◽  
Organic Pesticides

The present study was conducted to evaluate the sensitivity of the Vibrio fischeri bioluminescence inhibition test (Microtox® assay), and the standard acute Daphnia magna test; using 3 heavy metals, 3 organic pesticides, and their mixtures. In Daphnia tests, either at 24h or 30 min exposure times, the pattern of toxicity order for heavy metals was Cu ˃ Cd ˃ Pb. Chlorpyrifos-methyl was the highest toxic at 24h, while Triazophos was the highest toxic at 30 min exposure times. In the Microtox® test at 5 min exposure time, the estimated EC50 values were 4.20, 4.53 and 6.60 mg/L for Cu, Cd and Pb, respectively. At the same exposure time, the EC50 values of Triazophos, Chlorpyrifos-Me and Profenofos accounted to 1.76, 3.36 and 4.12 mg/L, respectively. Similar order of toxicity was obtained when tests were conducted at 15 min exposure time. The paired mixtures of pesticides, as well as the mixtures of Cu + Cd and Pb + Cd, showed potentiation effects, while the mixture of Cu + Pb showed additive effect against D. magna. The tertiary mixtures of the pesticides or the heavy metals reacted antagonistically. In the Microtox® assay, the heavy metal mixtures reacted antagonistically, while pesticide mixtures showed synergism. It was concluded that both Daphnia and Microtox® tests showed similar pattern of sensitivity to the single toxicants, but dissimilar pattern to the heavy metal mixtures. On the other side, using shorter exposure time (ca. 30 min) with Daphnia bioassay may enable us to held reliable comparisons with Microtox® results.

scholarly journals Ecotoxicological characterisation of exhaust particulates from diesel-powered light-duty vehicles

2013 ◽  
Vol 11 (12) ◽  
pp. 1954-1958 ◽  
Author(s):  
András Ács ◽  
Árpád Ferincz ◽  
Anikó Kovács ◽  
Beatrix Jancsek-Turóczi ◽  
András Gelencsér ◽  
...  
Keyword(s):  
Diesel Exhaust ◽  
Vibrio Fischeri ◽  
Total Carbon ◽  
Engine Emissions ◽  
Toxic Unit ◽  
Light Duty ◽  
Polycyclic Aromatic ◽  
Bioluminescence Inhibition ◽  
Ultrafine Particulate Matter ◽  
Light Duty Vehicles

AbstractDiesel exhaust is one of the major sources of fine and ultrafine particulate matter in urban air. Toxicity of diesel-powered engine emissions has been quite widely assessed, however, much less information is available on their ecotoxicity. In our study the kinetic version of the Vibrio fischeri bioluminescence inhibition bioassay, based on the ISO 21338:2010 standard, was used to characterise the ecotoxicity of diesel-powered cars. The method is sensitive enough to test the ecotoxic effect of the emission of individual vehicles. In general, significant positive correlation was found between ecotoxicity (expressed as Toxic Unit /TU/values) and total carbon (TC) as well as between TU and polycyclic aromatic hydrocarbon (PAH) concentrations.

Removal of ecotoxicity and COD from tank truck cleaning wastewater

2013 ◽  
Vol 68 (10) ◽  
pp. 2202-2207 ◽  
Author(s):  
Jan Dries ◽  
Wim De Schepper ◽  
Luc Geuens ◽  
Ronny Blust
Keyword(s):  
Activated Carbon ◽  
Acute Toxicity ◽  
Vibrio Fischeri ◽  
Oxygen Demand ◽  
Complete Removal ◽  
Tank Truck ◽  
Unit Operations ◽  
Bioluminescence Inhibition ◽  
Treatment Technologies ◽  
Treatment Step

Tank truck cleaning (TTC) activities generate highly complex wastewater. In a previous study, we found that a significant ecotoxic effect was still present in biologically treated TTC wastewater. The aim of the present study was therefore to investigate the removal of acute toxicity from TTC wastewater by a sequence of technologies routinely applied for industrial wastewater. Acute toxicity was assayed with the widely applied and standardized Vibrio fischeri bioluminescence inhibition test. During a 5-month period, raw wastewater was grab-sampled from a full-scale TTC company and treated by the different unit operations on a laboratory scale. Chemical pretreatment of the wastewater by coagulation with FeCl3 removed approx. 38% of the influent chemical oxygen demand (COD) and reduced the bioluminescence inhibition by 8%. Biological treatment with activated sludge subsequently removed another 77% of the remaining COD. This treatment step also reduced the bioluminescence inhibition but the removal efficiency varied strongly from 5 to 92% for the different samples. Powdered activated carbon almost completely removed the remaining COD and inhibition in all samples. The results suggest that conventional technologies did not suffice for complete removal of toxicity from TTC wastewater, and that advanced wastewater treatment technologies such as activated carbon are required for a satisfactory detoxification.

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