scholarly journals Bisphenol A interferes with first shell formation and development of the serotoninergic system in early larval stages of Mytilus galloprovincialis

2021 ◽  
Vol 758 ◽  
pp. 144003
Author(s):  
A. Miglioli ◽  
T. Balbi ◽  
L. Besnardeau ◽  
R. Dumollard ◽  
L. Canesi
Keyword(s):  
Bisphenol A ◽  
Mytilus Galloprovincialis ◽  
Serotoninergic System ◽  
Shell Formation ◽  
Larval Stages

scholarly journals Characterization of the main steps in first shell formation in Mytilus galloprovincialis : possible role of tyrosinase

2019 ◽  
Vol 286 (1916) ◽  
pp. 20192043 ◽  
Author(s):  
A. Miglioli ◽  
R. Dumollard ◽  
T. Balbi ◽  
L. Besnardeau ◽  
L. Canesi
Keyword(s):  
Time Course ◽  
Mytilus Galloprovincialis ◽  
Enzyme Inhibitor ◽  
Organic Matrix ◽  
Mrna Levels ◽  
Shell Formation ◽  
Larval Stages ◽  
Matrix Deposition ◽  
Shell Matrix

Bivalve biomineralization is a highly complex and organized process, involving several molecular components identified in adults and larval stages. However, information is still scarce on the ontogeny of the organic matrix before calcification occurs. In this work, first shell formation was investigated in the mussel Mytilus galloprovincialis . The time course of organic matrix and CaCO 3 deposition were followed at close times post fertilization (24, 26, 29, 32, 48 h) by calcofluor and calcein staining, respectively. Both components showed an exponential trend in growth, with a delay between organic matrix and CaCO 3 deposition. mRNA levels of genes involved in matrix deposition (chitin synthase; tyrosinase- TYR) and calcification (carbonic anhydrase; extrapallial protein) were quantified by qPCR at 24 and 48 hours post fertilization (hpf) with respect to eggs. All transcripts were upregulated across early development, with TYR showing highest mRNA levels from 24 hpf. TYR transcripts were closely associated with matrix deposition as shown by in situ hybridization. The involvement of tyrosinase activity was supported by data obtained with the enzyme inhibitor N-phenylthiourea. Our results underline the pivotal role of shell matrix in driving first CaCO 3 deposition and the importance of tyrosinase in the formation of the first shell in M. galloprovincialis .

scholarly journals Immune responses during the larval stages of Mytilus galloprovincialis: Metamorphosis alters immunocompetence, body shape and behavior

2013 ◽  
Vol 35 (2) ◽  
pp. 438-447 ◽  
Author(s):  
Pablo Balseiro ◽  
Rebeca Moreira ◽  
Rubén Chamorro ◽  
Antonio Figueras ◽  
Beatriz Novoa
Keyword(s):  
Immune Responses ◽  
Body Shape ◽  
Mytilus Galloprovincialis ◽  
Larval Stages ◽  
And Behavior

Dynamics of the parasite Marteilia refringens (Paramyxea) in Mytilus galloprovincialis and zooplankton populations in Alfacs Bay (Catalonia, Spain)

Parasitology ◽  
2007 ◽  
Vol 134 (11) ◽  
pp. 1541-1550 ◽  
Author(s):  
N. CARRASCO ◽  
I. LÓPEZ-FLORES ◽  
M. ALCARAZ ◽  
M. D. FURONES ◽  
F. C. J. BERTHE ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Zooplankton Community ◽  
Infection Process ◽  
Ostrea Edulis ◽  
Decapod Crustaceans ◽  
Intermediate Hosts ◽  
Experimental Transmission ◽  
Larval Stages ◽  
Delta De L'ebre ◽  
Euterpina Acutifrons

SUMMARYSince the first description of Marteilia refringens (Paramyxea) in flat oysters Ostrea edulis in 1968 in the Aber Wrach, Brittany (France), the life-cycle of this parasite has remained unknown. However, recent studies, conducted in the ‘claire’ system, have proposed the planktonic copepod Acartia grani as a potential intermediate host for the parasite. Nevertheless, experimental transmission of the parasite through the copepod has failed. Recent studies in this field have reported the presence of the parasite in zooplankton from the bays of the Delta de l'Ebre, a more complex and natural estuarine environment than that of the claire. As a result, 2 new Marteilia host species were proposed: the copepods Oithona sp. (Cyclopoida) and an indeterminate Harpaticoida. Consequently, the objective of the present work was to study the dynamics of Marteilia in the zooplankton community from one of the bays, Alfacs Bay, as well as the dynamics of the parasite in cultivated mussels during 1 complete year. Six different zooplankton taxa appeared to be parasitized by M. refringens, including copepods (3 Calanoida, Acartia discaudata, A. clausi and A. italica; 1 Cyclopoida, Oithona sp.; and 1 Harpacticoida, Euterpina acutifrons), and larval stages of decapod crustaceans (zoea larvae of Brachyura, probably Portumnus sp.). These taxa are thus proposed as new subjects for study, since they could be intermediate hosts in the infection process of mussels by Marteilia.

scholarly journals Impact of cationic polystyrene nanoparticles (PS-NH2) on early embryo development of Mytilus galloprovincialis: Effects on shell formation

Chemosphere ◽  
2017 ◽  
Vol 186 ◽  
pp. 1-9 ◽  
Author(s):  
Teresa Balbi ◽  
Giulia Camisassi ◽  
Michele Montagna ◽  
Rita Fabbri ◽  
Silvia Franzellitti ◽  
...  
Keyword(s):  
Embryo Development ◽  
Mytilus Galloprovincialis ◽  
Early Embryo ◽  
Shell Formation ◽  
Polystyrene Nanoparticles ◽  
Early Embryo Development

scholarly journals Upper lethal temperatures for the mussel Mytilus galloprovincialis (Lamarck, 1819), in central coast of Chile

2017 ◽  
Vol 43 (3) ◽  
pp. 473-483
Author(s):  
Andrés Mesas ◽  
Eduardo Tarifeño
Keyword(s):  
Geographic Distribution ◽  
Mytilus Galloprovincialis ◽  
Temperature Limit ◽  
Southern Chile ◽  
Physiological Tolerance ◽  
Larval Stages ◽  
Lethal Temperatures ◽  
Magellan Strait ◽  
Mediterranean Mussel ◽  
Physiological Background

The presence of Mytilus galloprovincialis, widely known as the Mediterranean mussel, have been genetically certified from Coliumo Bay to the Magellan Strait, in central and southern Chile, respectively. To correlate the geographic distribution of this species along the Chilean coast with its physiological tolerance to upper temperatures, experiments were carried out with adults and larval stages. Trochophore, umbonate veliger, and pre-metamorphic larvae were submitted to experimental temperatures from 17-33°C, during 24 h to determine the upper temperature limit. Meanwhile, adult experiments were carried with specimens having different previous physiological background: spring-summer acclimation (November-January), and fall-winter acclimation (August-October) and exposed to temperature range of 17-33°C for period of 24, 48, 72 and 96 h, to verify mortality and byssus formation. Results showed that at larvae stages, the 50% of mortality occurred at 27°C, with 100% mortality at 30°C. Adult stages reached the 55% mortality exposed for 48 h at 30°C and 100% mortality in 72 h at the same temperature. The byssus secretion become failing at 27°C and ceased at 30°C.

scholarly journals Impact of bisphenol A (BPA) on early embryo development in the marine mussel Mytilus galloprovincialis: Effects on gene transcription

2016 ◽  
Vol 218 ◽  
pp. 996-1004 ◽  
Author(s):  
Teresa Balbi ◽  
Silvia Franzellitti ◽  
Rita Fabbri ◽  
Michele Montagna ◽  
Elena Fabbri ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Embryo Development ◽  
Gene Transcription ◽  
Mytilus Galloprovincialis ◽  
Early Embryo ◽  
Marine Mussel ◽  
Early Embryo Development

Carbonic Anhydrase and Metazoan Biocalcification: A Focus on Molluscs

2016 ◽  
Vol 672 ◽  
pp. 151-157 ◽  
Author(s):  
Nathalie Le Roy ◽  
Daniel J. Jackson ◽  
Benjamin Marie ◽  
Paula Ramos-Silva ◽  
Frédéric Marin
Keyword(s):  
Carbonic Anhydrase ◽  
Sequence Data ◽  
Inorganic Ions ◽  
Organic Matrix ◽  
Expression Data ◽  
Shell Formation ◽  
Gastropod Mollusc ◽  
Larval Stages ◽  
Evolutionary Context ◽  
Reversible Hydration

Carbonic anhydrase is a super-family of metallo-enzymes (containing α, β, γ, ζ and δ-CA families) that catalyse the reversible hydration of carbon dioxide. Among their numerous functions, CAs - in particular that of the α-CA family - are known to play a key role in biocalcification processes, i.e., the ability to deposit calcium carbonate crystallites in a controlled manner to form exoskeletons. In the gastropod mollusc Haliotistuberculata – the European abalone – we identified two CA transcripts, htCA1 and htCA2, in the mantle, the calcifying organ responsible for shell formation from an extracellular organic matrix and a mixture of inorganic ions. Because these two transcripts are specifically expressed in the mantle, this suggests that the two corresponding CA isoforms may be directly involved in shell formation. In the present paper, whole mount in situ hybridization experiments performed on larval stages of H. tuberculata reveal the expression of htCA1 in cells associated with the statocysts, the sensory organs for gravity, while htCA2 is not expressed in these cells. We compile the activity and expression data for these two CAs in H.tuberculata and discuss these results in an evolutionary context using a simplified phylogeny from compiled CA sequence data of several metazoans. This shows that the evolution of this protein super-family has a complex history with origins at the dawn of the Phanerozoic.

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