Tunneling-induced groundwater depletion limits long-term growth dynamics of forest trees

Author(s):  
Hamid M. Behzad ◽  
Yongjun Jiang ◽  
Muhammad Arif ◽  
Chao Wu ◽  
QiuFang He ◽  
...  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Han Wang ◽  
Gloria M. Conover ◽  
Song-I Han ◽  
James C. Sacchettini ◽  
Arum Han

AbstractAnalysis of growth and death kinetics at single-cell resolution is a key step in understanding the complexity of the nonreplicating growth phenotype of the bacterial pathogen Mycobacterium tuberculosis. Here, we developed a single-cell-resolution microfluidic mycobacterial culture device that allows time-lapse microscopy-based long-term phenotypic visualization of the live replication dynamics of mycobacteria. This technology was successfully applied to monitor the real-time growth dynamics of the fast-growing model strain Mycobacterium smegmatis (M. smegmatis) while subjected to drug treatment regimens during continuous culture for 48 h inside the microfluidic device. A clear morphological change leading to significant swelling at the poles of the bacterial membrane was observed during drug treatment. In addition, a small subpopulation of cells surviving treatment by frontline antibiotics was observed to recover and achieve robust replicative growth once regular culture media was provided, suggesting the possibility of identifying and isolating nonreplicative mycobacteria. This device is a simple, easy-to-use, and low-cost solution for studying the single-cell phenotype and growth dynamics of mycobacteria, especially during drug treatment.


1999 ◽  
Vol 10 (6) ◽  
pp. 815-824 ◽  
Author(s):  
Kiyoshi Umeki ◽  
Kihachiro Kikuzawa

2016 ◽  
Vol 69 ◽  
pp. 64-67
Author(s):  
K. Mellow ◽  
P. Chettri ◽  
S. Kabir ◽  
R.L. McDougal ◽  
M.P. Cox ◽  
...  

Trichoderma cf atroviride is an endophytic soil fungus which has been the target of much research due to its plant growth promoting effects and use as a biocontrol agent. For specific bioactive strains of this species to be used with longlived forest trees such as Pinus radiata they must persist over the long term in the host root system. To investigate the persistence of specific T cf atroviride strains in the roots of P radiata unique regions were identified in the genomes of strains that were more than 997 identical by using next generation sequencing. Based on these unique regions probebased strainspecific quantitative PCR assays were developed. The assays can be used to test strain persistence in nursery and forestgrown seedlings inoculated with mixtures of T cf atroviride strains as well as in laboratory experiments to determine the effect of these strains on plant metabolism and defence.


2000 ◽  
Vol 151 (4) ◽  
pp. 99-106 ◽  
Author(s):  
Josef Senn

After excessive cutting in Swiss mountain forests and extirpation of most of the wildlife during the past centuries, efficient forestry and hunting laws allowed a wide regeneration of the forests and a rapid increase of ungulate populations in the present century. As a consequence, the impacts of ungulates on the vegetation became obvious. Regeneration of forest trees, however, is influenced not only by ungulates, but by a number of physical site factors and biotic impacts. As these impacts and their interactions vary extensively, regeneration is neither spatially nor temporally constant. Most of the presently used tree-regeneration methods, however, assume constant conditions, which renders a proper evaluation of tree regeneration in mountain forests and the role of ungulates impossible. Furthermore, the effect of this variation on forest development and forest functions is unknown with regard to the long term. While society requires a multipurpose mountain forest, structured at a small scale, wild ungulates use their habitat at a larger scale. This often leads to conflicts. Consequently,solutions including different scales are necessary. A lack of knowledge will, therefore, have to be met by research making data available to the practice as well as through coordinated investigations and experiments.


2018 ◽  
Vol 138 (1) ◽  
pp. 165-185 ◽  
Author(s):  
Hans Pretzsch ◽  
Miren del Río ◽  
Peter Biber ◽  
Catia Arcangeli ◽  
Kamil Bielak ◽  
...  

IAWA Journal ◽  
2000 ◽  
Vol 21 (3) ◽  
pp. 321-333 ◽  
Author(s):  
Josef Bauch ◽  
Oliver Dünisch

This study responds to the demand for recultivation of degraded land areas in Central Amazonia and the desire to add long-lived trees for high-quality timber production to polyculture plantation systems. Carapa guianensis Aubl. from two monocultures (ages 4 and 17 years) and a primary forest were studied. The growth dynamics of the 4-yearold trees revealed 11 to 15 increments marked by tangentially oriented vessel rows. By the 5th year, parenchyma bands indicate mature wood. Monthly labelling by pin-markers revealed short dormancy periods during the dry season. The early formation of mature wood is shown by the pattern of percentage composition of vessels, fibres, and parenchyma. Fibre length reaches its plateau of 1.45–1.59 mm at about four years. Fibre lengths and average density (0.63 g /cm3) of plantation-grown trees correspond to those of primary forest trees. According to this study, Carapa guianensis can be recommended for plantations in order to produce high-quality timber with a decorative heartwood.


PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173999 ◽  
Author(s):  
Larisa Avens ◽  
Lisa R. Goshe ◽  
Lewis Coggins ◽  
Donna J. Shaver ◽  
Ben Higgins ◽  
...  

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