How do environmentally friendly antifouling alkaloids affect marine fouling microbial communities?

Abstract The characterization of microbial community dynamics using genomic methods is rapidly expanding, impacting many fields including medical, ecological, and environmental research and applications. One of the biggest challenges for such studies is the isolation of environmental DNA (eDNA) from a variety of samples, diverse microbes, and widely variable community compositions. The current study developed environmentally friendly, user safe, economical, and high throughput eDNA extraction methods for mixed aquatic microbial communities and tested them using 16 s rRNA gene meta-barcoding. Five different lysis buffers including (1) cetyltrimethylammonium bromide (CTAB), (2) digestion buffer (DB), (3) guanidinium isothiocyanate (GITC), (4) sucrose lysis (SL), and (5) SL-CTAB, coupled with four different purification methods: (1) phenol-chloroform-isoamyl alcohol (PCI), (2) magnetic Bead-Robotic, (3) magnetic Bead-Manual, and (4) membrane-filtration were tested for their efficacy in extracting eDNA from recreational freshwater samples. Results indicated that the CTAB-PCI and SL-Bead-Robotic methods yielded the highest genomic eDNA concentrations and succeeded in detecting the core microbial community including the rare microbes. However, our study recommends the SL-Bead-Robotic eDNA extraction protocol because this method is safe, environmentally friendly, rapid, high-throughput and inexpensive.

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Ecologically safe protective coatings for transport

Вестник Российской академии наук ◽

10.31857/s0869-5873896593-602 ◽

2019 ◽

Vol 89 (6) ◽

pp. 593-602

Author(s):

V. Ya. Shevchenko ◽

O. A. Shilova ◽

T. A. Kochina ◽

L. D. Barinova ◽

O. V. Belyi

Keyword(s):

Protective Coatings ◽

Environmentally Friendly ◽

Climate Impacts ◽

Transport Infrastructure ◽

Marine Fouling ◽

New Methods ◽

Russian Academy Of Sciences ◽

Silicate Chemistry ◽

Academy Of Sciences

Ways to protect vehicles and transport infrastructure from the effects of negative climate impacts, corrosion, icing, radiation, marine fouling, and biodestruction are considered based on scientific developments of Russian Academy of Sciences’ Institute of Silicate Chemistry. New methods and approaches to develop environmentally friendly protective coatings are considered.

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Analysis of Marine Fouling Microbial Communities Adhering to Carboxyl Modified MWCNTs-Filled PDMS Coating Surface during the Initial Stage of Biofouling

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1061-1062.155 ◽

2014 ◽

Vol 1061-1062 ◽

pp. 155-161 ◽

Cited By ~ 2

Author(s):

Yuan Sun ◽

Shuang Liang ◽

Zhi Zhou Zhang

Keyword(s):

Microbial Communities ◽

Single Strand Conformation Polymorphism ◽

Marine Fouling ◽

Coating Surface ◽

Antifouling Coating ◽

Dominance Level ◽

Surface Nanostructure ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes ◽

Pdms Coating

In this study, a polydimethylsiloxane (PDMS) coating filled with low concentrations of selected carboxyl modified multi-walled carbon nanotubes (cMWCNTs) has been fabricated. The antifouling properties of cMWCNTs-filled PDMS coatings were tested and the diversity level and succession phenomenon of marine fouling microbial communities were analyzed using single strand conformation polymorphism (SSCP) method. Marine adhesion test showed that cMWCNTs-filled PDMS coating presented decent antifouling property. SSCP analysis revealed that fouling prokaryotic species on the cMWCNTs-filled PDMS coating presented high and stable diversity level while diversity and dominance level of fouling eukaryotic species were relatively low, similar to those on PDMS alone and other four antifouling coating surface without nanoparticles, suggesting that the main mechanism by which cMWCNTs-mediated surface nanostructure improves antifouling capacity may have no direct relationship with the patterns in the context of succession dynamics of prokaryotic and eukaryotic microbial communities.

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Favorable Soil Microbes for Sustainable Agriculture

Advances in Environmental Engineering and Green Technologies - Handbook of Research on Microbial Remediation and Microbial Biotechnology for Sustainable Soil ◽

10.4018/978-1-7998-7062-3.ch005 ◽

2021 ◽

pp. 135-157

Author(s):

Umair Riaz ◽

Laila Shahzad ◽

Wajiha Anum ◽

Anam Waheed

Keyword(s):

Nitrogen Fixation ◽

Plant Growth ◽

Sustainable Agriculture ◽

Microbial Communities ◽

Soil Microbes ◽

Crop Yields ◽

Environmentally Friendly ◽

Beneficial Microbes ◽

Synthetic Fertilizers ◽

Chemical Fungicides

Beneficial microbes are used as the best alternative against the synthetic fertilizers and pesticides. The beneficial microbes not only help with plant growth, nutrition uptake, nitrogen fixation, but also help in acquiring the ions, not freely available to plants to uptake; these microbes also guard the plants by secreting toxic chemicals by inducing defense systems against pathogens. These microbes can provide best choice to look forward to sustainable agriculture and sustainable ecosystem. The addition of soil inoculants in the form of microorganisms or bio stimulants promise more environmentally friendly approaches for augmenting crop yields. The crop becomes less reliant on chemical fungicides and herbicides as many strains of microorganism have abilities of controlling pests. In this chapter, the interaction of beneficial plant bacteria, bio stimulants, effects on native microbial communities, and bacteria influencing economically important crops are discussed.

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TOXIC METALS EXTRACTION DURING POTATO FERMENTATION

10.32006/eeep.2017.1.6267 ◽

2017 ◽

pp. 62-67 ◽

Cited By ~ 1

Author(s):

I. Sioma ◽

А. Tashyrev ◽

V. Govorukha ◽

Y. Prekrasna

Keyword(s):

Energy Source ◽

Microbial Communities ◽

Toxic Metals ◽

Total Concentration ◽

Environmentally Friendly ◽

Natural Soil ◽

Food Wastes ◽

Combined Action ◽

Metals Extraction

According to the thermodynamical prognosis developed by us the investigation of the interaction of Bacillus-Clostridium community and six representative metals was performed. To representative metals we refer: oxidisers (CrO42-), substitutes (Ni2+, Co2+) and combined action metals that combine the characteristics of both oxidizers and substitutes (Cu2+, Hg2+, Fe3+). The natural soil hydrogen-producing microbial communities appeared to be resistant to the metals in their high total concentration of 120 mg/l and also able to extract these metals from the solution (up to 96%). The obtained regularities are the base for the development of new combined biotechnologies, allowing to neutralize ecologically hazardous food wastes, treat metal contaminated sewages and simultaneously obtain environmentally friendly energy source – hydrogen.

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A peek in the micro-sized world: a review of design principles, engineering tools, and applications of engineered microbial community

Biochemical Society Transactions ◽

10.1042/bst20190172 ◽

2020 ◽

Vol 48 (2) ◽

pp. 399-409

Author(s):

Baizhen Gao ◽

Rushant Sabnis ◽

Tommaso Costantini ◽

Robert Jinkerson ◽

Qing Sun

Keyword(s):

Microbial Community ◽

Microbial Communities ◽

Environmental Remediation ◽

Real Life ◽

Design Principles ◽

Microbial Interactions ◽

Potential Applications ◽

New Gene ◽

Global Biogeochemical Cycles ◽

Synthetic Microbial Communities

Microbial communities drive diverse processes that impact nearly everything on this planet, from global biogeochemical cycles to human health. Harnessing the power of these microorganisms could provide solutions to many of the challenges that face society. However, naturally occurring microbial communities are not optimized for anthropogenic use. An emerging area of research is focusing on engineering synthetic microbial communities to carry out predefined functions. Microbial community engineers are applying design principles like top-down and bottom-up approaches to create synthetic microbial communities having a myriad of real-life applications in health care, disease prevention, and environmental remediation. Multiple genetic engineering tools and delivery approaches can be used to ‘knock-in' new gene functions into microbial communities. A systematic study of the microbial interactions, community assembling principles, and engineering tools are necessary for us to understand the microbial community and to better utilize them. Continued analysis and effort are required to further the current and potential applications of synthetic microbial communities.

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