Generation of human iPS cell line SKiPSc1 from healthy Human Neonatal Foreskin Fibroblast cells

BACKGROUND: With essential metals being studied and developed as anticancer agents, this study aims to explore the anticancer activity of Zn(II) arginine dithiocarbamate in the T47D and fibroblast cell lines. METHOD: The Zn(II) arginine dithiocarbamate complex was prepared by the in situ method and characterized using infra-red spectroscopy, melting point, X-ray fluorescence, and X-ray diffraction instruments. The complex compound was tested for its cytotoxicity to the T47D breast cancer and fibroblast cell lines. RESULTS: The cytotoxicity of the Zn(II) arginine dithiocarbamate complex to the T47D breast cancer cell line obtained IC50 = 3.16 μg/mL, while cisplatin obtained IC50 = 28.18 μg/mL. The cytotoxicity of the Zn(II) arginine dithiocarbamate complex to fibroblast cells obtained IC50 = 8709.63 μg/mL. CONCLUSION: The Zn(II) arginine dithiocarbamate complex has increased active cytotoxicity compared to cisplatin in inducing morphological changes in the T47D breast cancer cell line and is relatively non-toxic to fibroblast cells.

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Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions

Stem Cell Research ◽

10.1016/j.scr.2021.102193 ◽

2021 ◽

Vol 51 ◽

pp. 102193

Author(s):

Jose Inzunza ◽

Jonathan Arias-Fuenzalida ◽

Juan Segura-Aguilar ◽

Ivan Nalvarte ◽

Mukesh Varshney

Keyword(s):

Cell Line ◽

Human Fibroblasts ◽

Ips Cell ◽

Normal Human

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Establishment of a non-integrate iPS cell line (SDQLCHi023-A) from a patient with Xq25 microduplication syndrome carrying a 1.3 Mb hemizygote duplication at chrXq25

Stem Cell Research ◽

10.1016/j.scr.2020.102147 ◽

2020 ◽

pp. 102147

Author(s):

Xiaomeng Yang ◽

Chunhong Duan ◽

Haiyan Zhang ◽

Yue Li ◽

Jingyun Guan ◽

...

Keyword(s):

Cell Line ◽

Ips Cell

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Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa

Stem Cell Research ◽

10.1016/j.scr.2018.03.022 ◽

2018 ◽

Vol 29 ◽

pp. 139-142 ◽

Cited By ~ 2

Author(s):

Yonglong Guo ◽

Qiaolang Zeng ◽

Shiwei Liu ◽

Quan Yu ◽

Peiyuan Wang ◽

...

Keyword(s):

Retinitis Pigmentosa ◽

Cell Line ◽

Ips Cell ◽

Integrative Method

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Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system

Stem Cell Research ◽

10.1016/j.scr.2021.102580 ◽

2021 ◽

pp. 102580

Author(s):

Elena Romano ◽

Piera Trionfini ◽

Roberta Giampietro ◽

Ariela Benigni ◽

Susanna Tomasoni

Keyword(s):

Cell Line ◽

Ips Cell

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Generation of human iPS cell line IBCHi001-A from dentatorubral–pallidoluysian atrophy patient's fibroblasts

Stem Cell Research ◽

10.1016/j.scr.2019.101512 ◽

2019 ◽

Vol 39 ◽

pp. 101512 ◽

Cited By ~ 4

Author(s):

Emilia Kozlowska ◽

Agata Ciolak ◽

Marta Olejniczak ◽

Agnieszka Fiszer

Keyword(s):

Cell Line ◽

Ips Cell

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The influence of luteolin on expression of epithelial MUC1 mucin in human skin fibroblasts

Progress in Health Sciences ◽

10.5604/01.3001.0009.5050 ◽

2016 ◽

Vol 6 (2) ◽

pp. 64-69

Author(s):

J. Wosek ◽

I. Radziejewska ◽

E. Andrulewicz

Keyword(s):

Human Skin ◽

Real Time Pcr ◽

Skin Fibroblasts ◽

Pcr Analysis ◽

Fibroblast Cells ◽

Human Skin Fibroblasts ◽

Healthy Human ◽

Relative Level ◽

Muc1 Mucin ◽

Luteolin Treatment

Purpose: The membrane-anchored MUC1 mucin is typically expressed on normal and cancerous epithelial cells. Non-epithelial localization of this mucin is rare. However, the presence of MUC1 in human skin fibroblasts has been recently unexpectedly revealed. The aim of the study was to prove the expression of MUC1 mucin in human skin fibroblasts and the examine of the influence of luteolin on its expression. Materials and methods: ELISA tests and real-time PCR analysis were used to assess the expression of MUC1 mucin in fibroblast cells cocultured with 30 μM concentration of luteolin. Results: The expression of MUC1 was revealed in human skin fibroblasts. Luteolin decreased the relative level of mucin in cell lysates and media. Statistically significant decreased expression of MUC1 gene after luteolin treatment of fibroblasts cells was also revealed. Conclusion: Our results prove non-epithelial localization of MUC1 mucin. Luteolin inhibits the expression of MUC1 mucin in healthy human skin fibroblasts.

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