scholarly journals Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions

2021 ◽  
Vol 51 ◽  
pp. 102193
Author(s):  
Jose Inzunza ◽  
Jonathan Arias-Fuenzalida ◽  
Juan Segura-Aguilar ◽  
Ivan Nalvarte ◽  
Mukesh Varshney
Keyword(s):  
Cell Line ◽  
Human Fibroblasts ◽  
Ips Cell ◽  
Normal Human

scholarly journals LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA

1974 ◽  
Vol 140 (2) ◽  
pp. 494-507 ◽  
Author(s):  
M. E. Jobin ◽  
J. L. Fahey ◽  
Z. Price
Keyword(s):  
Multiple Myeloma ◽  
Cell Line ◽  
Human Fibroblasts ◽  
Myeloma Protein ◽  
Culture Viability ◽  
Feeder Layers ◽  
Normal Human ◽  
Stimulating Factor

Cell line LA-49, derived from pleural fluid cells of a patient with IgD multiple myeloma, was established in culture and maintained for more than 1 yr. The D-myeloma protein produced in culture was similar to the serum D-myeloma protein in electrophoretic mobility and in delta- and lambda-chain antigens. The plasma cell tumor culture, LA-49, differed from numerous immunoglobulin-producing B-lymphoblastoid cell lines established in this laboratory in: (a) Morphology (revealing various stages of maturation); (b) type of immunoglobulin produced (IgD vs. IgM, IgG, and/or, rarely, IgA); (c) growth characteristics (requirement of plasmacyte-stimulating factor); and (d) chromosomal features (polyploid vs. pseudodiploid). A growth factor was needed for cell division and maintenance of culture viability. This factor was supplied readily by irradiated feeder layers of normal human fibroblasts or conditional media from fibroblast cultures. Preliminary characterization of this factor revealed it to be a protein with a mol wt of approximately 150,000 daltons.

scholarly journals Cell cycle progression after cleavage failure

2004 ◽  
Vol 165 (5) ◽  
pp. 609-615 ◽  
Author(s):  
Yumi Uetake ◽  
Greenfield Sluder
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Mammalian Cells ◽  
Cell Cycle Progression ◽  
Human Fibroblasts ◽  
Human Cell Line ◽  
Cycle Progression ◽  
Binucleate Cells ◽  
Normal Human ◽  
Cleavage Failure

Failure of cells to cleave at the end of mitosis is dangerous to the organism because it immediately produces tetraploidy and centrosome amplification, which is thought to produce genetic imbalances. Using normal human and rat cells, we reexamined the basis for the attractive and increasingly accepted proposal that normal mammalian cells have a “tetraploidy checkpoint” that arrests binucleate cells in G1, thereby preventing their propagation. Using 10 μM cytochalasin to block cleavage, we confirm that most binucleate cells arrest in G1. However, when we use lower concentrations of cytochalasin, we find that binucleate cells undergo DNA synthesis and later proceed through mitosis in >80% of the cases for the hTERT-RPE1 human cell line, primary human fibroblasts, and the REF52 cell line. These observations provide a functional demonstration that the tetraploidy checkpoint does not exist in normal mammalian somatic cells.

scholarly journals Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions

2016 ◽  
Vol 17 (3) ◽  
pp. 474-478 ◽  
Author(s):  
Malin Kele ◽  
Kelly Day ◽  
Harriet Rönnholm ◽  
Jens Schuster ◽  
Niklas Dahl ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Fibroblasts ◽  
Ips Cell

scholarly journals Selective Cytotoxicity and Antioxidant Effects of Compounds from Dioscorea membranacea Rhizomes

2007 ◽  
Vol 2 (6) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Arunporn Itharat ◽  
Anuchit Plubrukan ◽  
Niwat Kaewpradub ◽  
Titima Chuchom ◽  
Pranee Ratanasuwan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Fibroblasts ◽  
Cancer Cell Lines ◽  
Cell Lung Carcinoma ◽  
Normal Human

Bioassay-guided isolation was used to separate the active ingredients of the ethanolic extract of Dioscorea membranacea by testing cytotoxic activity against three human cancer cell lines, i.e. large cell lung carcinoma (COR-L23), colon cell line (LS-174T) and breast cancer cell line (MCF-7), and two normal human cell lines, keratinocytes (SVK-14) and normal human fibroblasts (HF), using the SRB assay. The DPPH test for antioxidant activity was also employed, as was a test for LDH release as an indicator of damage to the cell membrane. Eight compounds were isolated, two naphthofuranoxepins (dioscorealides A [1] and B [2]), a 1,4-phenanthraquinone (dioscoreanone [3]), three steroids (β-sitosterol [4], stigmasterol [5] and β-D-sitosterol glucoside [8]) and two steroid saponins diosgenin-(3- O-α-L-rhamnopyranosyl (1→2)-β-D-glucopyranoside [6] and diosgenin 3- O-β-D-glucopyranosyl (1→3)-β-D-glucopyranoside [7]). Cytotoxic activity of 2, 3 and 6 was shown against three cancer cell lines, and 2 showed selective cytotoxic activity against lung and breast cancer, but was less active against the two normal cells, and was not toxic to cell membranes in the LDH assay. The highest antioxidant activity was shown by 3.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

scholarly journals Precursor-Boosted Production of Metabolites in Nasturtium officinale Microshoots Grown in Plantform Bioreactors, and Antioxidant and Antimicrobial Activities of Biomass Extracts

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4660
Author(s):  
Marta Klimek-Szczykutowicz ◽  
Michał Dziurka ◽  
Ivica Blažević ◽  
Azra Đulović ◽  
Małgorzata Miazga-Karska ◽  
...  
Keyword(s):  
Photosynthetic Pigments ◽  
Human Fibroblasts ◽  
Antimicrobial Activities ◽  
Precursor Feeding ◽  
Nasturtium Officinale ◽  
Qualitative And Quantitative ◽  
Normal Human ◽  
Inhibition Zones ◽  
Ferulic Acids ◽  
Antioxidant And Antimicrobial Activities

The study demonstrated the effects of precursor feeding on the production of glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in Nasturtium officinale microshoot cultures grown in Plantform bioreactors. It also evaluated the antioxidant and antimicrobial activities of extracts. L-phenylalanine (Phe) and L-tryptophan (Trp) as precursors were tested at 0.05, 0.1, 0.5, 1.0, and 3.0 mM. They were added at the beginning (day 0) or on day 10 of the culture. Microshoots were harvested after 20 days. Microshoots treated with 3.0 mM Phe (day 0) had the highest total GSL content (269.20 mg/100 g DW). The qualitative and quantitative profiles of the GSLs (UHPLC-DAD-MS/MS) were influenced by precursor feeding. Phe at 3.0 mM stimulated the best production of 4-methoxyglucobrassicin (149.99 mg/100 g DW) and gluconasturtiin (36.17 mg/100 g DW). Total flavonoids increased to a maximum of 1364.38 mg/100 g DW with 3.0 mM Phe (day 0), and polyphenols to a maximum of 1062.76 mg/100 g DW with 3.0 mM Trp (day 0). The precursors also increased the amounts of p-coumaric and ferulic acids, and rutoside, and generally increased the production of active photosynthetic pigments. Antioxidant potential increased the most with 0.1 mM Phe (day 0) (CUPRAC, FRAP), and with 0.5 mM Trp (day 10) (DPPH). The extracts of microshoots treated with 3.0 mM Phe (day 0) showed the most promising bacteriostatic activity against microaerobic Gram-positive acne strains (MIC 250–500 µg/mL, 20–21 mm inhibition zones). No extract was cytotoxic to normal human fibroblasts over the tested concentration range (up to 250 μg/mL).

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