A novel method based on the Seebeck effect for analyzing the behavior of the PLC band and type A serration

Yumoto and Dmochowski (Cancer Res.27, 2098 (1967)) reported the presence of mature and immature type C leukemia virus particles in leukemic organs and tissues such as lymph nodes, spleen, thymus, liver, and kidneys of SJL/J strain mice with Hodgki's-like disease or reticulum cell neoplasm (type B). In an attempt to ascertain the possibility that this neoplasia may be of viral origin, experiments with induction and transmission of this neoplasm were carried out using cell-free extracts of leukemic organs from an SJL/J strain mouse with spontaneous disease.It has been possible to induce the disease in low-leukemia BALB/c and C3HZB strain mice and serially transfer the neoplasia by cell-free extracts of leukemic organs of these mice. Histological examination revealed the neoplasia to be of either reticulum cell-type A or type B. Serial transfer is now in its fifth passage. In addition leukemic spleen from another SJL/J strain mouse with spontaneous reticulum cell neoplasm (type A) was set up in tissue culture and is now in its 141st serial passage in vitro. Preliminary results indicate that cell-free material of 39th tissue culture passage can reproduce neoplasia in BALB/c mice.

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Hepatitis A Antigen in Liver, Bile and Stool

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100115328 ◽

1975 ◽

Vol 33 ◽

pp. 340-341

Author(s):

D.R. Jackson ◽

J.H. Hoofnagle ◽

A.N. Schulman ◽

J.L. Dienstag ◽

R.H. Purcell ◽

...

Keyword(s):

Hepatitis A ◽

Liver Enzymes ◽

Hepatitis A Virus ◽

Type A ◽

Initial Study ◽

Virus Like Particle ◽

Elevated Liver Enzymes ◽

Ag Particles ◽

Ag Particle ◽

Human Stool

Using immune electron microscopy Feinstone et. al. demonstrated the presence of a 27 nm virus-like particle in acute-phase stools of patients with viral hepatitis, type A, These hepatitis A antigen (HA Ag) particles were aggregated by convalescent serum from patients with type A hepatitis but not by pre-infection serum. Subsequently Dienstag et. al. and Maynard et. al. produced acute hepatitis in chimpanzees by inoculation with human stool containing HA Ag. During the early acute disease, virus like particles antigenically, morphologically and biophysically identical to the human HA Ag particle were found in chimpanzee stool. Recently Hilleman et. al. have described similar particles in liver and serum of marmosets infected with hepatitis A virus (HAV). We have investigated liver, bile and stool from chimpanzees and marmosets experimentally infected with HAV. In an initial study, a chimpanzee (no.785) inoculated with HA Ag-containing stool developed elevated liver enzymes 21 days after exposure.

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Detection of Viral Antigens in Mouse and Rat Tumor Cells by Immunoelectron Microscopy Following Periodate-Lysine-Paraformaldehyde (PLP) Fixation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010009124x ◽

1976 ◽

Vol 34 ◽

pp. 252-253

Author(s):

Y. Ohtsuki ◽

G. Seman ◽

J. M. Bowen ◽

M. Scanlon ◽

L. Dmochowski

Keyword(s):

Immunoelectron Microscopy ◽

Mammary Tumor ◽

Type A ◽

Rabbit Serum ◽

Virus Particles ◽

Viral Antigens ◽

Culture Cells ◽

Mouse Mammary Tumor ◽

Tumor Virus ◽

Immunoperoxidase Labeling

Recently, periodate-lysine-paraformaldehyde (PLP) fixation was reported for immunoelectron microscopy (1). In PLP fixation, carbohydrates are oxidized by periodate and cross-linked by lysine; paraformaldehyde stabilizes proteins and lipids. By using PLP fixation, intracytoplasmic type A viral antigens have been previously demonstrated by immunoperoxidase labeling (2). In the present study, PLP fixation has been applied for the detection of the same antigens in mouse mammary tumor culture cells by both immunoferritin and immunoperoxidase methods. Rabbit anti-intracytoplasmic type A virus serum (anti-A), kindly provided by Dr. M. Muller (3), rabbit anti-strain A mouse mammary tumor virus (anti-MMTV) and preimmune rabbit serum as control were used to detect viral antigens in cells of C3H/HeJ strain mouse mammary tumor culture. Attempts have been also made to demonstrate peroxidase labeling of type C virus particles in frozen sections of an SD-MSV-induced NZB rat bone tumor tissue by rabbit anti-MuLV serum.

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The removal and morphology of intact biofilms from implanted venous access devices

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141470 ◽

1995 ◽

Vol 53 ◽

pp. 1020-1021

Author(s):

M.A. Gregory ◽

G.P. Hadley

Keyword(s):

Patient Selection ◽

Surgical Oncology ◽

Venous Access ◽

Common Procedure ◽

Indwelling Catheters ◽

Careful Patient ◽

Novel Method ◽

The Subject ◽

Vascular Catheters ◽

Venous Access Devices

The insertion of implanted venous access systems for children undergoing prolonged courses of chemotherapy has become a common procedure in pediatric surgical oncology. While not permanently implanted, the devices are expected to remain functional until cure of the primary disease is assured. Despite careful patient selection and standardised insertion and access techniques, some devices fail. The most commonly encountered problems are colonisation of the device with bacteria and catheter occlusion. Both of these difficulties relate to the development of a biofilm within the port and catheter. The morphology and evolution of biofilms in indwelling vascular catheters is the subject of ongoing investigation. To date, however, such investigations have been confined to the examination of fragments of biofilm scraped or sonicated from sections of catheter. This report describes a novel method for the extraction of intact biofilms from indwelling catheters.15 children with Wilm’s tumour and who had received venous implants were studied. Catheters were removed because of infection (n=6) or electively at the end of chemotherapy.

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