Selective Preparation of Substituted Maltooligosaccharides through Enzymatic Hydrolysis of Substituted β-Cyclodextrins by Bacterial α-Amylase (Saccharifying Type). A Novel Method for Determining Regiochemical Structure of Disubstituted β-Cyclodextrin

Abstract We describe a novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure. Under the conditions of the assay, the enzymatic hydrolysis can be completed in less than 10 min by the combined action of a microbial lipase and a protease. We have been able to demonstrate complete hydrolysis of triglycerides by thin-layer chromatography of the reaction products, by recovery of glycerol from sera of known triglycerides content, and by comparison of triglyceride assays on a number of sera assayed by our method vs. the AutoAnalyzer procedure. The hydrolysis is directly coupled to the enzymatic determination of glycerol, and is followed through absorbance changes at 340 nm. The assay is simple, rapid, and requires only 50 µl or less of sample. Because the enzymes used do not release glycerol from other compounds in serum, the hydrolysis can be considered specific for triglycerides.

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Microwave-assisted enzymatic hydrolysis of starch

10.3390/ecsoc-13-00159 ◽

2009 ◽

Cited By ~ 1

Author(s):

Marcin Lukasiewicz ◽

Anna Osowiec ◽

Magdalena Marciniak

Keyword(s):

Enzymatic Hydrolysis ◽

Microwave Assisted ◽

Hydrolysis Of

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Analyses of enzymatic hydrolysis of bagasse cane of sugar for obtention of ethanol employing a cocktail of natives commercials enzymes.

10.3390/mol2net-04-05300 ◽

2018 ◽

Author(s):

Ángel Batallas ◽

Erenio González ◽

Carmen Salvador ◽

Jonathan Villavicencio ◽

Humberto González Gavilánez ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Hydrolysis Of

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Faculty Opinions recommendation of Enzymatic hydrolysis of pneumococcal capsular polysaccharide renders the bacterium vulnerable to host defense.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733387483.793547056 ◽

2018 ◽

Author(s):

Mattias Collin

Keyword(s):

Enzymatic Hydrolysis ◽

Host Defense ◽

Capsular Polysaccharide ◽

Hydrolysis Of

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Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

Current Nanoscience ◽

10.2174/1573413714666180611081759 ◽

2019 ◽

Vol 15 (3) ◽

pp. 296-303 ◽

Cited By ~ 5

Author(s):

Swapnil Gaikwad ◽

Avinash P. Ingle ◽

Silvio Silverio da Silva ◽

Mahendra Rai

Keyword(s):

Catalytic Activity ◽

Enzymatic Hydrolysis ◽

Immobilized Enzyme ◽

Free Enzyme ◽

Magnetic Nanomaterials ◽

Sugar Production ◽

Cellulase Enzyme ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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