scholarly journals DNA finger printing of S. Mutans present in the saliva of caries active children and those associated with intellectual disability – An RAPD analysis

2019 ◽  
Vol 31 (4) ◽  
pp. 424-430 ◽  
Author(s):  
A.R. Prabhakar ◽  
Gudla Sreeja ◽  
Saraswatthi V. Naik
Keyword(s):  
Intellectual Disability ◽  
Rapd Analysis ◽  
Finger Printing ◽  
Dna Finger Printing

PERFORMANCE OF TORIA (BRASSICA COMPESTRIS SYN. RAPA L.) VARIETY: RAJ VIJAY TORIA-2 IN CENTRAL, EASTERN AND NORTH-EASTERN STATES OF INDIA UNDER RAINFED CONDITION

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
VIMLESH KUMAR TIWARI
Keyword(s):  
Seed Yield ◽  
Rainfed Condition ◽  
Significant Difference ◽  
North Eastern ◽  
Finger Printing ◽  
Quantitative And Qualitative Traits ◽  
Central Eastern ◽  
Dna Finger Printing ◽  
Mature Seeds ◽  
Weight Trait

Performance of developed genotype RMT 08-2 was evaluated in central, eastern and north-eastern states of India under rain-fed condition for quantitative and qualitative traits. It gave highest seed yield over checks in zone III and V. Morphologically plants were erect, medium spreading in nature and primary branches with dichotomous habit. Plants height ranged from 107124 (cm) which matured in 82-112 days. Mature seeds were round in shape and blackish brown in colour. No significant difference between RVT-2 and checks were observed for test weight trait. An average oil yield 485 (kg/h) was recorded over 7 places which was 10% higher than both checks i.e. 14.12% and 11.24% under AICRP trials. Maximum seed yield was obtained on farmers field during 2013-14 and 2014-15 which was 1500 (kg/h) and 1215 (kg/h) that is 33.42% and 26.30% respectively over farmers own seeds. At Morena center, highest seed yield (1753 kg/h) over Bhawani (1512 kg/h) was 15.94% higher than check whereas RVT-2 gave 2245 (kg/h) against Bhawani (1975 kg/h) which was 13.67% higher. DNA finger printing indicated that primers PUT-19, PUT-96, PUT-149, PUT-169, PUT-181 and PUT-271 are useful in generating unique profile of RVT-2 containing 27 bands for its discrimination from other varieties.

scholarly journals Does monozygotic twinning occur in mice?

1995 ◽  
Vol 66 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Anne McLaren ◽  
Paul Molland ◽  
Esther Signer
Keyword(s):  
Dna Fingerprinting ◽  
Monozygotic Twin ◽  
Statistical Evidence ◽  
Genetic Loci ◽  
Induced Ovulation ◽  
Monozygotic Twinning ◽  
Finger Printing ◽  
Dna Finger Printing ◽  
Mouse System

SummaryPublished reports suggest that the incidence of monozygotic twinning in women is increased after hormonally induced ovulation. Since some statistical evidence exists to indicate that monozygotic twinning may also occur in mice, we attempted to devise a mouse system in which the incidence of such twinning could be compared after spontaneous versus hormonally induced ovulation, in order to analyse the developmental basis of such an effect. We used phenotypic identity in litters segregating for ten genetic loci (not all independent) to indicate possible twin pairs. DNA fingerprinting using three human minisatellite probes was then performed blind on these pairs and on sibling controls. From a total of over 2000 mice born, 40 apparently identical pairs were identified, on which DNA finger-printing was successfully carried out on 35 pairs. All proved to be derived from different zygotes. We conclude that monozygotic twin pairs are either extremely rare in the stock of mice that we studied, or have such reduced viability that their chance of surviving to weaning is low.

Genomic and outer membrane protein diversity fingerprints of siderophore producing fluorescent Pseudomonas spp. using RAPD, Rep-PCR and SDS-PAGE profiling

Biologia ◽  
2015 ◽  
Vol 70 (9) ◽  
pp. 1150-1158 ◽  
Author(s):  
Nellickal Subramanian Jayamohan ◽  
Shiragambi Hanamanthagouda Manohar ◽  
Belur Satyan Kumudini
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Binary Data ◽  
Chemical Characterization ◽  
Plant Growth Promoting Rhizobacteria ◽  
Fluorescent Pseudomonas ◽  
Sds Page ◽  
Finger Printing ◽  
Dna Finger Printing

Abstract Fluorescent Pseudomonas (FP) is an intrinsic group of plant growth promoting rhizobacteria (PGPR) which also has the potential to control phytopathogens. In the present study sixteen FP isolates were subjected to siderophore production and siderotyping. Four DNA finger printing analysis markers (RAPD, ERIC, BOX A1R, REP and GTG5) were used to assess their genetic diversity through DNA finger printing analysis. Outer membrane protein (OMP) homology of FP was carried out by SDS-PAGE analysis. Chemical characterization showed that the siderophores produced were hydroxamate in nature. Production of siderophores ranged from 21.73 ± 2.18 to 182.03 ± 1.66 μg mL−1 after 72 hr incubation. Maximum production was shown by the isolate M139. Dendrogram obtained from the combined binary data of 10 RAPD primers classified FPs into two distinct clusters. Except for M140, all the isolates from maize rhizosphere were grouped into the same cluster. Among three Rep-PCR analyses, REP primer showed maximum diversity in banding pattern. Combined binary data of three Rep primers also classified FP into two clusters as in RAPD and protein electrophenogram of the OMP. To the best of our knowledge this is the first report on genomic and OMP disparity among hydroxamate producing FPs.

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