scholarly journals A DNA Finger Printing Technique Analysis Used in Herbal Formulations

Author(s):  
Jaya Lakshmi Kundla ◽  
Vinodkumar K. ◽  
Sai Lakshmi K.
2018 ◽  
Vol 24 (2) ◽  
Author(s):  
VIMLESH KUMAR TIWARI

Performance of developed genotype RMT 08-2 was evaluated in central, eastern and north-eastern states of India under rain-fed condition for quantitative and qualitative traits. It gave highest seed yield over checks in zone III and V. Morphologically plants were erect, medium spreading in nature and primary branches with dichotomous habit. Plants height ranged from 107124 (cm) which matured in 82-112 days. Mature seeds were round in shape and blackish brown in colour. No significant difference between RVT-2 and checks were observed for test weight trait. An average oil yield 485 (kg/h) was recorded over 7 places which was 10% higher than both checks i.e. 14.12% and 11.24% under AICRP trials. Maximum seed yield was obtained on farmers field during 2013-14 and 2014-15 which was 1500 (kg/h) and 1215 (kg/h) that is 33.42% and 26.30% respectively over farmers own seeds. At Morena center, highest seed yield (1753 kg/h) over Bhawani (1512 kg/h) was 15.94% higher than check whereas RVT-2 gave 2245 (kg/h) against Bhawani (1975 kg/h) which was 13.67% higher. DNA finger printing indicated that primers PUT-19, PUT-96, PUT-149, PUT-169, PUT-181 and PUT-271 are useful in generating unique profile of RVT-2 containing 27 bands for its discrimination from other varieties.


1995 ◽  
Vol 66 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Anne McLaren ◽  
Paul Molland ◽  
Esther Signer

SummaryPublished reports suggest that the incidence of monozygotic twinning in women is increased after hormonally induced ovulation. Since some statistical evidence exists to indicate that monozygotic twinning may also occur in mice, we attempted to devise a mouse system in which the incidence of such twinning could be compared after spontaneous versus hormonally induced ovulation, in order to analyse the developmental basis of such an effect. We used phenotypic identity in litters segregating for ten genetic loci (not all independent) to indicate possible twin pairs. DNA fingerprinting using three human minisatellite probes was then performed blind on these pairs and on sibling controls. From a total of over 2000 mice born, 40 apparently identical pairs were identified, on which DNA finger-printing was successfully carried out on 35 pairs. All proved to be derived from different zygotes. We conclude that monozygotic twin pairs are either extremely rare in the stock of mice that we studied, or have such reduced viability that their chance of surviving to weaning is low.


1999 ◽  
Vol 79 ◽  
pp. 236
Author(s):  
Misa Yamada ◽  
Mitsuhiko Yamada ◽  
Yuji Kiuchi ◽  
Keinosuke Nara ◽  
Kazutaka Momose ◽  
...  

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