DNA finger printing by oligonucleotide probes specific for simple repeats

1986 ◽  
Vol 74 (3) ◽  
Author(s):  
S. Ali ◽  
C.R. M�ller ◽  
J.T. Epplen
Keyword(s):  
Oligonucleotide Probes ◽  
Finger Printing ◽  
Dna Finger Printing ◽  
Simple Repeats

scholarly journals Heterogeneity in the Sxr (sex-reversal) locus of the mouse as revealed by synthetic probes

1988 ◽  
Vol 51 (3) ◽  
pp. 239-246 ◽  
Author(s):  
Jörg T. Epplen ◽  
Roland Studer ◽  
Anne McLaren
Keyword(s):  
Recombination Rate ◽  
Restriction Enzymes ◽  
Sex Reversal ◽  
Variant Form ◽  
Oligonucleotide Probes ◽  
Male Mice ◽  
Hybridization Pattern ◽  
Unequal Recombination ◽  
Finger Printing ◽  
Dna Finger Printing

SummaryThe sex-reversal mutation, Sxr and a variant form, Sxr′ have been established on the inbred C57BL/6Mcl background by repeated backcrossing to form the CB and CB′ strains, respectively. DNAs of normal XY, XX Sxr and XX Sxr′ as well as XY Sxr and XY Sxr′ carrier male mice have been digested with the restriction enzymes Hae III and Hinf I and electrophoresed. The DNAs show many common but also differing hybridization bands with synthetic oligonucleotide probes. In XY Sxr (and XY Sxr′) carrier males, the hybridization patterns of normal XY and those of XX Sxr (and XX Sxr′) males are simply superimposed. Individual differing bands can be categorized by their differential hybridization behaviour to the (GATA)4, (GACA)4, (GATA)2 GACA (GATA)2 and (GATA)3 (GACA)2 probes. In general, the hybridization patterns are regularly inherited. In addition to the predominant pattern in each strain, one additional XX Sxr and one additional XX Sxr′ hybridization pattern was observed: the additional pattern in the CB strain was transmitted (via variant XY Sxr carriers) while the secondary XX Sxr′ pattern in the CB′ strain could only be observed once. Thus ‘DNA finger printing’ with oligonucleotide probes can successfully be used to discriminate the DNAs of normal Y chromosomes, XX Sxr and XX Sxr′ variants as well as XY Sxr and XY Sxr′ carrier mice. Implications of the comparatively high unequal recombination rate involving the murine Y chromosome are discussed, as well as possible mechanisms.

PERFORMANCE OF TORIA (BRASSICA COMPESTRIS SYN. RAPA L.) VARIETY: RAJ VIJAY TORIA-2 IN CENTRAL, EASTERN AND NORTH-EASTERN STATES OF INDIA UNDER RAINFED CONDITION

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
VIMLESH KUMAR TIWARI
Keyword(s):  
Seed Yield ◽  
Rainfed Condition ◽  
Significant Difference ◽  
North Eastern ◽  
Finger Printing ◽  
Quantitative And Qualitative Traits ◽  
Central Eastern ◽  
Dna Finger Printing ◽  
Mature Seeds ◽  
Weight Trait

Performance of developed genotype RMT 08-2 was evaluated in central, eastern and north-eastern states of India under rain-fed condition for quantitative and qualitative traits. It gave highest seed yield over checks in zone III and V. Morphologically plants were erect, medium spreading in nature and primary branches with dichotomous habit. Plants height ranged from 107124 (cm) which matured in 82-112 days. Mature seeds were round in shape and blackish brown in colour. No significant difference between RVT-2 and checks were observed for test weight trait. An average oil yield 485 (kg/h) was recorded over 7 places which was 10% higher than both checks i.e. 14.12% and 11.24% under AICRP trials. Maximum seed yield was obtained on farmers field during 2013-14 and 2014-15 which was 1500 (kg/h) and 1215 (kg/h) that is 33.42% and 26.30% respectively over farmers own seeds. At Morena center, highest seed yield (1753 kg/h) over Bhawani (1512 kg/h) was 15.94% higher than check whereas RVT-2 gave 2245 (kg/h) against Bhawani (1975 kg/h) which was 13.67% higher. DNA finger printing indicated that primers PUT-19, PUT-96, PUT-149, PUT-169, PUT-181 and PUT-271 are useful in generating unique profile of RVT-2 containing 27 bands for its discrimination from other varieties.

scholarly journals Does monozygotic twinning occur in mice?

1995 ◽  
Vol 66 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Anne McLaren ◽  
Paul Molland ◽  
Esther Signer
Keyword(s):  
Dna Fingerprinting ◽  
Monozygotic Twin ◽  
Statistical Evidence ◽  
Genetic Loci ◽  
Induced Ovulation ◽  
Monozygotic Twinning ◽  
Finger Printing ◽  
Dna Finger Printing ◽  
Mouse System

SummaryPublished reports suggest that the incidence of monozygotic twinning in women is increased after hormonally induced ovulation. Since some statistical evidence exists to indicate that monozygotic twinning may also occur in mice, we attempted to devise a mouse system in which the incidence of such twinning could be compared after spontaneous versus hormonally induced ovulation, in order to analyse the developmental basis of such an effect. We used phenotypic identity in litters segregating for ten genetic loci (not all independent) to indicate possible twin pairs. DNA fingerprinting using three human minisatellite probes was then performed blind on these pairs and on sibling controls. From a total of over 2000 mice born, 40 apparently identical pairs were identified, on which DNA finger-printing was successfully carried out on 35 pairs. All proved to be derived from different zygotes. We conclude that monozygotic twin pairs are either extremely rare in the stock of mice that we studied, or have such reduced viability that their chance of surviving to weaning is low.

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