Polymorphism identification in ovine KISS1R/GPR54 gene among pure and crossbreeds of Iranian sheep

One of the main economical problems of sheep breeding is that continual production is not possible due to the seasonality of animals. Today, genes, mutations that may develop aseasonality in sheep are extensively researched in order to make the establishment of populations capable of aseasonal estrus possible.According to the current knowledge, the GPR54 gene participates in the GnRH release from the hypothalamus, and thus in the alteration of the reproduction state of the organism. Our research is aimed to determine whether the GPR54 gene has allele variations that influence the proneness to aseasonal estrus in sheep in a different extent. Therefore, the GPR54 gene sequence of sheep is first examined.

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Association of Kiss1 and GPR54 Gene Polymorphisms with Polycystic Ovary Syndrome among Sri Lankan Women

BioMed Research International ◽

10.1155/2019/6235680 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Umayal Branavan ◽

Kajan Muneeswaran ◽

W. S. S. Wijesundera ◽

Anoma Senanayake ◽

N. Vishvanath Chandrasekharan ◽

...

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Reproductive Age ◽

Sri Lankan ◽

Nucleotide Polymorphisms ◽

Ovary Syndrome ◽

Significant Difference ◽

Study Population ◽

Marginal Association ◽

Gpr54 Gene

Polycystic ovary syndrome (PCOS) is the commonest endocrine disorder affecting women of reproductive age. Its aetiology, though yet unclear, is presumed to have an oligogenic basis interacting with environmental factors. Kisspeptins are peptide products of Kiss1 gene that control the hypothalamic pituitary (HPG) axis by acting via G protein-coupled receptor known as GPR54. There is paucity of data on the role of Kiss1 and GPR54 gene in PCOS. We aimed to identify the polymorphisms in Kiss1 and GPR54 genes and explore their association with serum kisspeptin levels among Sri Lankan women with well-characterized PCOS. Consecutive women with PCOS manifesting from adolescence (n=55) and adult controls (n=110) were recruited. Serum kisspeptin and testosterone levels were determined by ELISA method. Whole gene sequencing was performed to identify the polymorphisms in Kiss1 and GPR54 genes. Serum kisspeptin and testosterone concentrations were significantly higher in women with PCOS than controls: kisspeptin 4.873nmol/L versus 4.127nmol/L; testosterone 4.713nmol/L versus 3.415 nmol/L, p<0.05. Sequencing the GPR54 gene revealed 5 single nucleotide polymorphisms (SNPs), rs10407968, rs1250729403, rs350131, chr19:918686, and chr19:918735, with two novel SNPs (chr19:918686 and chr19:918735), while sequencing the Kiss1 gene revealed 2 SNPs, rs5780218 and rs4889. All identified SNPs showed no significant difference in frequency between patients and controls. GPR54 gene rs350131 polymorphism (G/T) was detected more frequently in our study population. The heterozygous allele (AG) of GPR54 gene novel polymorphism chr19:918686 showed a marginal association with serum kisspeptin levels (p=0.053). Genetic variations in GPR54 and Kiss1 genes are unlikely to be associated with PCOS among Sri Lankan women manifesting from adolescence. Meanwhile the heterozygous allele of chr19:918686 is probably associated with serum kisspeptin concentrations, which suggests a potential role in the aetiology of PCOS.

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Novel DNA variation of GPR54 gene in familial central precocious puberty

Italian Journal of Pediatrics ◽

10.1186/s13052-019-0601-6 ◽

2019 ◽

Vol 45 (1) ◽

Cited By ~ 1

Author(s):

Nosrat Ghaemi ◽

Martha Ghahraman ◽

Samaneh Noroozi Asl ◽

Rahim Vakili ◽

Fatemeh Fardi Golyan ◽

...

Keyword(s):

Precocious Puberty ◽

Central Precocious Puberty ◽

Dna Variation ◽

Gpr54 Gene

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Faculty Opinions recommendation of The GPR54 gene as a regulator of puberty.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.14276942.793561649 ◽

2019 ◽

Author(s):

Tony Plant

Keyword(s):

Gpr54 Gene

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Characterisation of KiSS1R gene and Non genetic factors affecting reproductive traits in Indian goats

Indian Journal of Animal Research ◽

10.18805/ijar.b-3597 ◽

2019 ◽

Author(s):

Siddhant Sekhar Sahoo ◽

Chinmoy Mishra ◽

Stuti Tanaya Mohanty ◽

Rakesh Kaushik ◽

P. K. Rout ◽

...

Keyword(s):

Sexual Maturity ◽

Genetic Factors ◽

Reproductive Traits ◽

Factors Affecting ◽

Polymorphic Pattern ◽

Nucleotide Sequence Alignment ◽

Goat Population ◽

Black Bengal Goat ◽

Gpr54 Gene ◽

Ncbi Blast

This study was undertaken to explore the genetic polymorphism in the KiSS1R (GPR54) gene from 80 Black Bengal, 50 Ganjam and 20 Raighar goat. Each of the sampled goats was recorded for its reproductive traits. The genomic DNA was isolated from the collected blood samples. The target 3’ UTR comprising of 246 bp fragment of KiSS1R gene was successfully amplified using the specific primer. Amplified samples were subjected for HRM analysis followed by sequencing. The nucleotide sequence alignment with the retrieved DNA sequence from NCBI BLAST confirmed absence of polymorphic pattern in KiSS1R gene in 3’ UTR. However, in the studied populations breed had significant effect on littersize, kidding interval and age at sexual maturity. It was found that age at sexual maturity and kidding interval were the highest in Ganjam goat population as compared to Raighar and Black Bengal goat population. Litter size was found highest in Black Bengal goat.

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