Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy

2011 ◽  
Vol 156 (1) ◽  
pp. 410-415 ◽  
Author(s):  
Lin Xue ◽  
Zhangjian Fang ◽  
Guoping Li ◽  
Huanhuan Wang ◽  
Hua Jiang
Keyword(s):  
Aqueous Solution ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Zinc Ions ◽  
Two Photon Microscopy ◽  
Two Photon

A novel peptide-based fluorescent chemosensor for measuring zinc ions using different excitation wavelengths and application in live cell imaging

2015 ◽  
Vol 3 (17) ◽  
pp. 3617-3624 ◽  
Author(s):  
Peng Wang ◽  
Jiang Wu ◽  
Panpan Zhou ◽  
Weisheng Liu ◽  
Yu Tang
Keyword(s):  
Aqueous Solution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Fluorescent Chemosensor ◽  
Zinc Ions ◽  
Enhanced Fluorescence ◽  
Chelation Enhanced Fluorescence

A novel peptide-based fluorescent chemosensor containing both tryptophan and a dansyl fluorophore has been designed to detect Zn2+ in 100% aqueous solution and living cells via two pathways including fluorescence resonance energy transfer and chelation enhanced fluorescence.

Fluorescent sensors based on [12]aneN3-modified BODIPY: Discrimination of different biological thiols in aqueous solution and living cells

2016 ◽  
Vol 24 (7) ◽  
pp. 1550-1559 ◽  
Author(s):  
Yong-Guang Gao ◽  
Ying Zhang ◽  
You-Di Shi ◽  
Hu-Jun Hao ◽  
Bing Gong ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Living Cells ◽  
Fluorescent Sensors

Switchable and selective detection of Zn2+ or Cd2+ in living cells based on 3′-O-substituted arrangement of benzoxazole-derived fluorescent probes

2014 ◽  
Vol 50 (56) ◽  
pp. 7514-7516 ◽  
Author(s):  
Yongqian Xu ◽  
Liangliang Xiao ◽  
Shiguo Sun ◽  
Zhichao Pei ◽  
Yuxin Pei ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Selective Detection

Two benzoxazole-derived ESIPT fluorescent sensors E1 and E2 show highly selective detection of Zn2+ and Cd2+, respectively, in aqueous solution and living cells.

A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging

2020 ◽  
Vol 44 (26) ◽  
pp. 11342-11348 ◽  
Author(s):  
Ming-Xuan Hou ◽  
Liu-Yi Liu ◽  
Kang-Nan Wang ◽  
Xi-Juan Chao ◽  
Rong-Xue Liu ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Living Cells ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Molecular Rotor ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A two-photon fluorescent probe was developed for detecting mitochondrial viscosity during apoptosis of living cells by two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM) with good selectivity and highly biocompatible.

A molecular probe based on pyrimidine imidazole derivatives for stable super-resolution endoplasmic reticulum imaging in living cells

2018 ◽  
Vol 42 (18) ◽  
pp. 14725-14728 ◽  
Author(s):  
Ling Hu ◽  
Sajid Hussain ◽  
Tianyan Liu ◽  
Yuanzhen Yue ◽  
Jiejie Liu ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Super Resolution ◽  
Living Cells ◽  
Molecular Probes ◽  
Living Systems ◽  
Molecular Probe ◽  
Imidazole Derivatives ◽  
Two Photon Microscopy ◽  
Two Photon

Multi-functional florescent dyes capable of acting as molecular probes in living systems under two-photon microscopy, as well as super-resolution nanoscopy, are of great interest.

scholarly journals Mitochondrial-Targeted Two-Photon Fluorescent Probes for Zinc Ions,H2O2, and Thiols in Living Tissues

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Hwan Myung Kim ◽  
Bong Rae Cho
Keyword(s):  
Reactive Oxygen Species ◽  
Chemical Species ◽  
Primary Site ◽  
Zinc Ions ◽  
Oxygen Species ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Cell Tissue ◽  
Ideal Tool ◽  
Living Tissues

Mitochondria provide the energy of the cells and are the primary site of oxygen consumption and the major source of reactive oxygen species. In mitochondria, metal ions and glutathione play vital roles in maintaining their structure and the redox environment. To understand their roles in mitochondria, it is crucial to monitor each of these chemical species in the mitochondria at the cell, tissue, and organism levels. An ideal tool for such purpose is the use of two-photon microscopy (TPM). Until recently, however, there has been no report on the two-photon (TP) probes suitable for such applications. In this paper, we summarize the mitochondria-targeted TP probes for Zn2+, H2O2, and thiols, as well as their bioimaging applications.

scholarly journals Visualizing lipid structure and raft domains in living cells with two-photon microscopy

2003 ◽  
Vol 100 (26) ◽  
pp. 15554-15559 ◽  
Author(s):  
K. Gaus ◽  
E. Gratton ◽  
E. P. W. Kable ◽  
A. S. Jones ◽  
I. Gelissen ◽  
...  
Keyword(s):  
Living Cells ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Lipid Structure ◽  
Raft Domains
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