A highly fluorescent sensor based on hybrid nanoparticles for selective determination of furosemide in aqueous medium

Abstract A derivative UV-spectrophotometric analytical procedure was developed for determination of three 4-quinolone antibacterials: norfloxacin (NFX), ciprofloxacin (CFX), and sparfloxacin (SFX). The method depends on the complexation of Cu(II) with the studied compounds in aqueous medium. A third order, measurement was applied for their quantification. A linear correlation was established between the amplitude of the peak and concentration for all the studied drugs in the range of 15–80, 35–120, and 200–700 ng/mL, with minimum detectability (S/N = 2) of 1.0, 1.3, and 5.1 ng/mL for NFX, CFX, and SFX, respectively. The method was successfully applied for accurate, sensitive, and selective determination of the studied drugs in bulk and tablets formulation with average percentage recoveries of 99.22 ± 0.55 to 100.33 ± 1.60. The results obtained were favorably compared with those of the reference method. The method was also used to determine sparfloxacin in spiked human plasma and urine. The results obtained were satisfactory, accurate, and precise.

Download Full-text

Computer-aided design and synthesis of CdTe@SiO2core-shell molecularly imprinted polymers as a fluorescent sensor for the selective determination of sulfamethoxazole in milk and lake water

Journal of Separation Science ◽

10.1002/jssc.201601180 ◽

2017 ◽

Vol 40 (5) ◽

pp. 1091-1098 ◽

Cited By ~ 9

Author(s):

Wanzhen Xu ◽

Yingchun Wang ◽

Weihong Huang ◽

Luan Yu ◽

Yanfei Yang ◽

...

Keyword(s):

Molecularly Imprinted Polymers ◽

Computer Aided Design ◽

Fluorescent Sensor ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Selective Determination ◽

Design And Synthesis ◽

Computer Aided ◽

Aided Design

Download Full-text

Synthesis of New Quinoline-Conjugated Calixarene as a Fluorescent Sensor for Selective Determination of Cu2+ Ion

Journal of Fluorescence ◽

10.1007/s10895-021-02749-6 ◽

2021 ◽

Author(s):

Serkan Sayin

Keyword(s):

Fluorescent Sensor ◽

Selective Determination

Download Full-text

Schiff-Based Fluorescent-ON Sensor L Synthesis and Its Application for Selective Determination of Cerium in Aqueous Media

Journal of Sensors ◽

10.1155/2020/2192584 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Maria Sadia ◽

Jehangir Khan ◽

Robina Naz ◽

Muhammad Zahoor ◽

Ezzat Khan ◽

...

Keyword(s):

Metal Ion ◽

Limit Of Detection ◽

Fluorescent Sensor ◽

Aqueous Media ◽

High Sensitivity ◽

Transition Metal Ions ◽

Acetonitrile Solution ◽

Selective Determination ◽

Sensitivity And Selectivity

In the present study, a fluorescent sensor L for sensing of Ce3+ ion was designed and characterized by XRD, 1HNMR, and FTIR. Its fluorescence behavior towards metal ion was examined by fluorescence spectroscopy. Chelation-enhanced fluorescence was shown by the sensor L upon interaction with Ce3+ ion. This fluorescent sensor exhibits high sensitivity and selectivity towards Ce3+ ion in acetonitrile solution, forming 2 : 1 (L : M) complex as determined by Job’s plot. Association constant was found to be 1×107 M−1 estimated from the Benesi-Hildebrand plot. No significant interference was observed in the presence of other studied alkali, alkaline, and transition metal ions. A rapid response was observed when employed for the determination of Ce3+ ion in spiked water samples with a limit of detection equal to 3.4×10−8 M.

Download Full-text

Fluorescent sensor for selective determination of copper ion based on N-acetyl-l-cysteine capped CdHgSe quantum dots

Biosensors and Bioelectronics ◽

10.1016/j.bios.2013.11.008 ◽

2014 ◽

Vol 54 ◽

pp. 311-316 ◽

Cited By ~ 18

Author(s):

Qingqing Wang ◽

Xiangyang Yu ◽

Guoqing Zhan ◽

Chunya Li

Keyword(s):

Quantum Dots ◽

Fluorescent Sensor ◽

Copper Ion ◽

Selective Determination ◽

Determination Of Copper

Download Full-text

Selective determination of mitochondrial chelatable iron in viable cells with a new fluorescent sensor

Biochemical Journal ◽

10.1042/bj3620137 ◽

2002 ◽

Vol 362 (1) ◽

pp. 137-147 ◽

Cited By ~ 59

Author(s):

Frank PETRAT ◽

Daniela WEISHEIT ◽

Martina LENSEN ◽

Herbert de GROOT ◽

Reiner SUSTMANN ◽

...

Keyword(s):

Time Course ◽

Fluorescent Sensor ◽

Rat Hepatocytes ◽

Intact Cells ◽

Free System ◽

Selective Determination ◽

Viable Cells ◽

Pyridoxal Isonicotinoyl Hydrazone ◽

Cultured Rat Hepatocytes

Mitochondrial chelatable ('redox-active') iron is considered to contribute to several human diseases, but has not yet been characterized in viable cells. In order to determine this iron pool, we synthesized a new fluorescent indicator, rhodamine B-[(1,10-phenanthrolin-5-yl)aminocarbonyl]benzyl ester (RPA). In a cell-free system, RPA fluorescence was strongly and stoichiometrically quenched by Fe2+. RPA selectively accumulated in the mitochondria of cultured rat hepatocytes. The intramitochondrial RPA fluorescence was quenched when iron was added to the cells in a membrane-permeant form. It increased when the mitochondrial chelatable iron available to the probe was experimentally decreased by the membrane-permeant transition metal chelators pyridoxal isonicotinoyl hydrazone and 1,10-phenanthroline. The concentration of mitochondrial chelatable iron in cultured rat hepatocytes, quantified from the increase in RPA fluorescence after addition of pyridoxal isonicotinoyl hydrazone, was found to be 12.2±4.9μM. Inhibition of haem synthesis with succinylacetone did not alter the signal obtained in hepatocytes, but a rapid increase in the concentration of mitochondrial chelatable iron was observed in human erythroleukaemia K562 cells. In conclusion, RPA enables the selective determination of the highly physiologically and pathophysiologically interesting mitochondrial pool of chelatable iron in intact cells and to record the time course of alterations of this pool.

Download Full-text