A Novel Electrochemical Biosensor for Bisphenol A Detection Based on Engineered Escherichia coli Cells with a Surface-display of Tyrosinase

2021 ◽  
pp. 131063
Author(s):  
Shuai Zhao ◽  
Tuoyu Zhou ◽  
Aman Khan ◽  
Zhengjun Chen ◽  
Pu Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bisphenol A ◽  
Electrochemical Biosensor ◽  
Surface Display ◽  
Escherichia Coli Cells ◽  
Engineered Escherichia Coli ◽  
Bisphenol A Detection

Development of bisphenol A-removing recombinant Escherichia coli by monomeric and dimeric surface display of bisphenol A-binding peptide

2017 ◽  
Vol 41 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Murali kannan Maruthamuthu ◽  
Jiyeon Hong ◽  
Kulandaisamy Arulsamy ◽  
Sivachandiran Somasundaram ◽  
SoonHo Hong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bisphenol A ◽  
Surface Display ◽  
Recombinant Escherichia Coli ◽  
Binding Peptide

scholarly journals Laccase Electrochemical Biosensor Based on Graphene-Gold/Chitosan Nanocomposite Film for Bisphenol A Detection

2020 ◽  
Vol 16 (5) ◽  
pp. 570-579
Author(s):  
Fuzi M. Fartas ◽  
Jaafar Abdullah ◽  
Nor A. Yusof ◽  
Yusran Sulaiman ◽  
Mohd I. Saiman ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Electrochemical Biosensor ◽  
Limit Of Detection ◽  
Modified Electrodes ◽  
Electrochemical Performances ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Laccase Enzyme ◽  
Bisphenol A Detection ◽  
Screen Printed

Background: Bisphenol A (BPA) is considered one of the most common chemicals that could cause environmental endocrine disrupting. Therefore, there is an increasing demand for simple, rapid and sensitive methods for BPA detection that result from BPA leaching into foods and beverages from storage containers. Herein, a simple laccase electrochemical biosensor was developed for the determination of BPA based on Screen-Printed Carbon Electrode (SPCE) modified graphenegold/ chitosan. The synergic effect of graphene-gold/chitosan nanocomposite as electrode modifier greatly facilitates electron-transfer processes between the electrolyte and laccase enzyme, thus leads to a remarkably improved sensitivity for bisphenol A detection. Methods: In this study, laccase enzyme is immobilized onto the Screen-Printed Carbon Electrode (SPCE) modified Graphene-Decorated Gold Nanoparticles (Gr-AuNPs) with Chitosan (Chit). The surface structure of nanocomposite was studied using different techniques including Field Emission Scanning Microscopy (FESEM), TRANSMISSION Electron Microscopy (TEM), Raman spectroscopy and Energy Dispersive X-ray (EDX). Meanwhile, the electrochemical performances of the modified electrodes were studied using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). Results: The developed laccase biosensor offered excellent analytical performance for the detection of BPA with a sensitivity of 0.271 μA/μM and Limit of Detection (LOD) of 0.023 μM, respectively. Moreover, the constructed biosensor showed good reproducibility, selectivity and stability towards BPA. The sensor has been used to detect BPA in a different type of commercial plastic products as a real sample and satisfactory result was obtained when compared with the HPLC method. Conclusion: The proposed electrochemical laccase biosensor exhibits good result which is considered as a promising candidate for a simple, rapid and sensitive method especially in the resource- limited condition.

scholarly journals Oxidative stress sensitivity of engineered Escherichia coli cells with a reduced genome

2011 ◽  
Vol 322 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Yumi Iwadate ◽  
Hirofumi Honda ◽  
Haruhiko Sato ◽  
Masayuki Hashimoto ◽  
Jun-ichi Kato
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Stress Sensitivity ◽  
Reduced Genome ◽  
Escherichia Coli Cells ◽  
Engineered Escherichia Coli

Studies of operational variables in batch mode for genetically engineered Escherichia coli cells containing penicillin acylase

1993 ◽  
Vol 15 (12) ◽  
pp. 1070-1073 ◽  
Author(s):  
S. Bhattacharya ◽  
V.S. Gupta ◽  
A.A. Prabhune ◽  
H. SivaRaman ◽  
M. Debnath ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Penicillin Acylase ◽  
Genetically Engineered ◽  
Batch Mode ◽  
Escherichia Coli Cells ◽  
Operational Variables ◽  
Engineered Escherichia Coli
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