Background:
Bisphenol A (BPA) is considered one of the most common chemicals that
could cause environmental endocrine disrupting. Therefore, there is an increasing demand for simple,
rapid and sensitive methods for BPA detection that result from BPA leaching into foods and beverages
from storage containers. Herein, a simple laccase electrochemical biosensor was developed for
the determination of BPA based on Screen-Printed Carbon Electrode (SPCE) modified graphenegold/
chitosan. The synergic effect of graphene-gold/chitosan nanocomposite as electrode modifier
greatly facilitates electron-transfer processes between the electrolyte and laccase enzyme, thus leads
to a remarkably improved sensitivity for bisphenol A detection.
Methods:
In this study, laccase enzyme is immobilized onto the Screen-Printed Carbon Electrode
(SPCE) modified Graphene-Decorated Gold Nanoparticles (Gr-AuNPs) with Chitosan (Chit). The
surface structure of nanocomposite was studied using different techniques including Field Emission
Scanning Microscopy (FESEM), TRANSMISSION Electron Microscopy (TEM), Raman spectroscopy
and Energy Dispersive X-ray (EDX). Meanwhile, the electrochemical performances of the modified
electrodes were studied using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV).
Results:
The developed laccase biosensor offered excellent analytical performance for the detection of
BPA with a sensitivity of 0.271 μA/μM and Limit of Detection (LOD) of 0.023 μM, respectively.
Moreover, the constructed biosensor showed good reproducibility, selectivity and stability towards
BPA. The sensor has been used to detect BPA in a different type of commercial plastic products as a
real sample and satisfactory result was obtained when compared with the HPLC method.
Conclusion:
The proposed electrochemical laccase biosensor exhibits good result which is
considered as a promising candidate for a simple, rapid and sensitive method especially in the resource-
limited condition.
Laccase Electrochemical Biosensor Based on Graphene-Gold/Chitosan Nanocomposite Film for Bisphenol A Detection
