scholarly journals Laccase Electrochemical Biosensor Based on Graphene-Gold/Chitosan Nanocomposite Film for Bisphenol A Detection

2020 ◽  
Vol 16 (5) ◽  
pp. 570-579
Author(s):  
Fuzi M. Fartas ◽  
Jaafar Abdullah ◽  
Nor A. Yusof ◽  
Yusran Sulaiman ◽  
Mohd I. Saiman ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Electrochemical Biosensor ◽  
Limit Of Detection ◽  
Modified Electrodes ◽  
Electrochemical Performances ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Laccase Enzyme ◽  
Bisphenol A Detection ◽  
Screen Printed

Background: Bisphenol A (BPA) is considered one of the most common chemicals that could cause environmental endocrine disrupting. Therefore, there is an increasing demand for simple, rapid and sensitive methods for BPA detection that result from BPA leaching into foods and beverages from storage containers. Herein, a simple laccase electrochemical biosensor was developed for the determination of BPA based on Screen-Printed Carbon Electrode (SPCE) modified graphenegold/ chitosan. The synergic effect of graphene-gold/chitosan nanocomposite as electrode modifier greatly facilitates electron-transfer processes between the electrolyte and laccase enzyme, thus leads to a remarkably improved sensitivity for bisphenol A detection. Methods: In this study, laccase enzyme is immobilized onto the Screen-Printed Carbon Electrode (SPCE) modified Graphene-Decorated Gold Nanoparticles (Gr-AuNPs) with Chitosan (Chit). The surface structure of nanocomposite was studied using different techniques including Field Emission Scanning Microscopy (FESEM), TRANSMISSION Electron Microscopy (TEM), Raman spectroscopy and Energy Dispersive X-ray (EDX). Meanwhile, the electrochemical performances of the modified electrodes were studied using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). Results: The developed laccase biosensor offered excellent analytical performance for the detection of BPA with a sensitivity of 0.271 μA/μM and Limit of Detection (LOD) of 0.023 μM, respectively. Moreover, the constructed biosensor showed good reproducibility, selectivity and stability towards BPA. The sensor has been used to detect BPA in a different type of commercial plastic products as a real sample and satisfactory result was obtained when compared with the HPLC method. Conclusion: The proposed electrochemical laccase biosensor exhibits good result which is considered as a promising candidate for a simple, rapid and sensitive method especially in the resource- limited condition.

scholarly journals Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4324 ◽  
Author(s):  
Nurul Talib ◽  
Faridah Salam ◽  
Yusran Sulaiman
Keyword(s):  
Graphene Oxide ◽  
Limit Of Detection ◽  
Food Product ◽  
Electrochemical Immunosensor ◽  
Growth Promoter ◽  
Carbon Electrode ◽  
Electrochemical Assay ◽  
Sensor Platform ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).

Metallic Film Modified Screen-Printed Carbon Electrode for Determination of 17α-Methyltestosterone

2019 ◽  
Vol 824 ◽  
pp. 182-189
Author(s):  
Chim Math ◽  
Wijitar Dungchai ◽  
Sudtida Pliankarom Thanasupsin
Keyword(s):  
Modified Electrode ◽  
Limit Of Detection ◽  
Diffusion Mechanism ◽  
Modified Electrodes ◽  
Active Surface ◽  
Deposition Potential ◽  
Active Surface Area ◽  
Male Population ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

17α-methyltestosterone (MT) is a synthetic androgen. It is used widely for inducing an all-male population of Nile tilapia (Oreochromis niloticus). In this work, the detection of MT was conducted using screen-printed carbon electrodes (SPCE). These were a bare electrode, a bismuth modified electrode (Bi-SPCE) and an antimony modified electrode (Sb-SPCE). The successful electrode modification was confirmed by scanning electron microscopy. The electroanalytical performance of the SPCE modified electrodes for MT detection was examined by cyclic voltammetry. The highest active surface area of 1.073x10-4 cm2 was obtained on Sb-SPCE. This indicates that Sb-SPCE can enhance the sensitivity of MT detection better than the bare-SPCE and the Bi-SPCE. The Sb-SPCE showed a linear response for MT concentrations ranging from 2 to 8 mg.L-1. The sensitivity obtained from the slope of a calibration curve was -0.452 mA.mol-1.L-1 in a Britton-Robinson buffer pH 4.0 containing Sb 16 mg.L-1 with deposition potential and deposition time of 1 V and 90 seconds, respectively. A linear relationship between the square root of the scan rate and the peak current revealed that mass transfer of MT to the electrode was driven by a diffusion mechanism. The limit of detection was found to be 1 mg.L-1.

Efficient wide range electrochemical bisphenol-A sensor by self-supported dendritic platinum nanoparticles on screen-printed carbon electrode

2018 ◽  
Vol 255 ◽  
pp. 2800-2808 ◽  
Author(s):  
Kyubin Shim ◽  
Jeonghun Kim ◽  
Mohammed Shahabuddin ◽  
Yusuke Yamauchi ◽  
Md. Shahriar A. Hossain ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Platinum Nanoparticles ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Wide Range ◽  
Screen Printed

scholarly journals Uric Acid Biosensor Based on Biofilm of L. plantarum using Screen-Printed Carbon Electrode Modified by Magnetite

Al-Kimia ◽  
2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Dian Siska Rahma Fatonah ◽  
Deden Saprudin ◽  
Dyah Iswantini ◽  
Novik Nurhidayat
Keyword(s):  
Uric Acid ◽  
Limit Of Detection ◽  
Clinical Analysis ◽  
Carbon Electrode ◽  
Range Limit ◽  
Good Electrocatalytic Activity ◽  
Screen Printed Carbon Electrode ◽  
Limit Of Quantitation ◽  
The Stability ◽  
Screen Printed

Biosensor based on biofilm of L. plantarum has been successfully done for determination of uric acid in human urine compared with colorimetric enzymatic produced relative error of less than 5%. L. plantarum has uricase activity to react with uric acid, to maintain the stability of bacteria forming themselves into biofilms. Magnetite is known to increase sensitivity of the biosensor. The combination of magnetite-polyethylene glycol (Fe3O4-PEG) was used to modify the surface of Screen-Printed Carbon Electrode modified (SPCE) and the resulting modified electrode (biofilm/Fe3O4/PEG/SPCE) displayed good electrocatalytic activity to the oxidation of UA. The composition of biofilms with optical density 1, magnetite 100 mgmL-1 and PEG 3% v / v were able to increase the current up to 48% in 4mM of UA. The biosensor with an optimum composition produced good linearity with a concentration range, limit of detection, limit of quantitation, sensitivity, and repeatability were found to be 0.1 - 4.3 mM, 70 µM,  234 µM, 25.392 µA mM-1, 2.38%, respectively. This biosensor stable up to 49 days of measurement with the remaining activity was 90.70% and selective for interference compounds such as salt, urea, glucose, ascorbic acid. This method has a good stability, sensitivity, and potential application in clinical analysis. Keyword: biofilm, biosensor, L. plantarum, magnetite, uric acid.

Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique

2021 ◽  
Vol 24 (2) ◽  
pp. 43-50
Author(s):  
Wulan Tri Wahyuni ◽  
Rudi Heryanto ◽  
Eti Rohaeti ◽  
Achmad Fauzi ◽  
Budi Riza Putra
Keyword(s):  
Uric Acid ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
Electrochemical Measurement ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Screen Printed Carbon Electrode ◽  
Limit Of Quantitation ◽  
Screen Printed

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.

Detection of Bisphenol A on a Screen-Printed Carbon Electrode in CTAB Micellar Medium

2010 ◽  
Vol 43 (18) ◽  
pp. 2823-2836 ◽  
Author(s):  
Michelle Fernanda Brugnera ◽  
Magno Aparecido Gonçalves Trindade ◽  
Maria Valnice Boldrin Zanoni
Keyword(s):  
Bisphenol A ◽  
Carbon Electrode ◽  
Micellar Medium ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Hydrothermal synthesis of NiFe2O4 nanoparticles as an efficient electrocatalyst for the electrochemical detection of bisphenol A

2020 ◽  
Vol 44 (19) ◽  
pp. 7698-7707 ◽  
Author(s):  
Ganesh Kesavan ◽  
Nandini Nataraj ◽  
Shen-Ming Chen ◽  
Li-Heng Lin
Keyword(s):  
Hydrothermal Synthesis ◽  
Bisphenol A ◽  
Electrochemical Detection ◽  
Selective Detection ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Nife2o4 Nanoparticles ◽  
Screen Printed

In this study, the sensitive and selective detection of bisphenol A (BPA) was achieved using a screen-printed carbon electrode (NFO/SPCE) modified with hydrothermally synthesized NiFe2O4 nanoparticles.

scholarly journals Penentuan Hidrokuinon dalam Sampel Krim Pemutih Wajah secara Voltammetri Menggunakan Screen Printed Carbon Electrode (SPCE)

2015 ◽  
pp. 97-102
Author(s):  
Ani Mulyasuryani ◽  
Alfita Savitri
Keyword(s):  
Limit Of Detection ◽  
Differential Pulse ◽  
Electrode System ◽  
Carbon Electrode ◽  
Peak Potential ◽  
Screen Printed Carbon Electrode ◽  
The Face ◽  
High Pulse ◽  
Pulse Voltammetry ◽  
Screen Printed

Hydroquinone in whitening face cream has been banned since 2008, but is still found facial bleaching creams containing hydroquinone. Therefore, in this study have been developed voltammetric method for the determination hydroquinone in face whitening cream. This study has been carried out optimization of pH and measurement conditions. Optimizationof pH has been done in cyclic voltammetry, while the optimization of measurement carried out by differential pulse voltammetry. In this study, using a screen printed carbon electrode with a three electrode system. The results showed that the increase in pH causes a decrease in the anodic peak potential (Epa) of hydroquinone. The optimum conditions resulted at pH 2 in which the anodic current (Ipa) is the highest. The optimum condition resulted at high pulse 200 mV and scan rate at 15 mV/sec. The linear regression concentration is 1-100 μM, limit of detection is 0.015 μM and sensitivity is 0.0652 μM/µA. The results showed that the concentration of hydroquinone in the face whitening cream samples ranged from 0 to 0.02%DOI :http://dx.doi.org/10.15408/jkv.v0i0.3145.

scholarly journals SIMULTANEOUS DETERMINATION OF DOPAMINE IN THE PRESENCE OF ASCORBIC ACID AND URIC ACID BY ELECTROCHEMICALLY OXIDIZED SCREEN PRINTED CARBON ELECTRODE

2018 ◽  
Vol 55 (5B) ◽  
pp. 78
Author(s):  
Nguyen Xuan Viet
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Limit Of Detection ◽  
Phosphate Buffer Solution ◽  
Electrochemical Technique ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Simple Method ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

This research reported the simple method to determine of dopamine (DA) in the simultaneous presence of ascorbic acid (AA) and uric acid (UA). Three – electrode system manufactured by screen printing method was used due to its disposal and low cost. The screen printed carbon electrode (SPCE) was oxidized by electrochemical technique in acid medium. The capacity of oxidized electrode for selective detection of dopamine was confirmed in a sufficient amount of ascorbic acid and uric acid. The large separated peaks of DA from ascorbic acid and uric acid are observed. The peak separation between UA and DA, DA and AA was 110 mV and 160 mV, respectively. The bare SPCE cannot determine simultaneously AA and DA due to the overlap peaks of AA and DA around 0.2 V vs AgCl/Ag. This sensor also exhibited good sensitivity to DA with limit of detection 100 nM in phosphate buffer solution.

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