Au nanoparticles/SnO2/ZnIn2S4-based biosensor for photoelectrochemical/electrochemical dual-signal detection of RNase A by combining the enzymolysis of DNA probe and the generation of molybdophosphate precipitate

2021 ◽  
pp. 131251
Author(s):  
Ying Ying ◽  
Min Zhou ◽  
Si Dai ◽  
Ming Ma ◽  
Wenfang Deng ◽  
...  
2018 ◽  
Vol 27 (6) ◽  
pp. 593-604
Author(s):  
Daae Lee ◽  
Hyeong Cheol Moon ◽  
Bao-Tram Tran ◽  
Dae-Hyuk Kwon ◽  
Yong Hee Kim ◽  
...  

Author(s):  
Gary Bassell ◽  
Robert H. Singer

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.


Author(s):  
K. S. Zaychuk ◽  
M. H. Chen ◽  
C. Hiruki

Wheat spot mosaic (WSpM), which frequently occurs with wheat streak mosaic virus was first reported in 1956 from Alberta. Singly isolated, WSpM causes chlorotic spots, chlorosis, stunting, and sometimes death of the wheat plants. The vector responsible for transmission is the eriophyid mite, Eriophyes tulipae Kiefer. The examination of leaf ultrastructure by electron microscopy has revealed double membrane bound bodies (DMBB’s) 0.1-0.2 μm in diameter. Dispersed fibrils within these bodies suggested the presence of nucleic acid. However, neither ribosomes characteristic of bacteria, mycoplasma and the psittacosis group of organisms nor an electron dense core characteristic of many viruses was commonly evident.In an attempt to determine if the DMBB’s contain nucleic acids, RNase A, DNase I, and lactoferrin protein were conjugated with 10 nm colloidal gold as previously described. Young root and leaf tissues from WSpM-affected wheat plants were fixed in glutaraldehyde, postfixed in osmium tetroxide,and embedded in Spurr’s resin.


2005 ◽  
Vol 112 (1) ◽  
pp. 268-279 ◽  
Author(s):  
Richard B. Anderson ◽  
Michael E. Doherty ◽  
Neil D. Berg ◽  
Jeff C. Friedrich
Keyword(s):  

1995 ◽  
Vol 40 (10) ◽  
pp. 972-972
Author(s):  
Jerome R. Busemeyer

2001 ◽  
Vol 6 (1) ◽  
pp. 35-48 ◽  
Author(s):  
Michaela Kiernan ◽  
Helena C. Kraemer ◽  
Marilyn A. Winkleby ◽  
Abby C. King ◽  
C. Barr Taylor

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