A simplified procedure for predicting the soil amplification factor based on the strain compatible period

In order to create a soil amplification map for Lima, Peru, parameters that correlate best with amplification are examined. Shallow shear wave velocity profiles estimated from MASW measurements at 105 sites were used to provide amplification factor AvTF. AVs10 seems to be the best value for estimating amplification in Lima from the data available. We have attempted to create AVs10 map correlating three parameters – elevation, H/V peak period, and soil type. From this AVs10 map, we have estimated an amplification map for Lima.

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A rapid silver-impregnation technique on ultra-thin sections for Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147053 ◽

1993 ◽

Vol 51 ◽

pp. 242-243

Author(s):

K. Chien ◽

R.C. Heusser ◽

M.L. Jones ◽

R.L. Van de Velde

Keyword(s):

Electron Microscopy ◽

Silver Nitrate ◽

Silver Impregnation ◽

Sodium Borate ◽

Renal Diseases ◽

Distilled Water ◽

Thin Sections ◽

Impregnation Technique ◽

Centrifuge Tube ◽

Simplified Procedure

Silver impregnation techniques have been used for the demonstration of the complex carbohydrates in electron microscopy. However, the silver stains were believed to be technically sensitive and time consumming to perform. Currently, due to the need to more specifically evaluate immune complex for localization in certain renal diseases, a simplified procedure in conjunction with the use of the microwave has been developed and applied to renal and other biopsies. The procedure is as follows:Preparation of silver methenamine solution:1. 15ml graduated, clear polystyrene centrifuge tube (Falcon, No. 2099) was rinsed once with distilled water.2. 3% hexamethylene tetramine (methenamine) was added into the centrifuge tube to the 6ml mark.3. 3% silver nitrate was added slowly to the methenamine to the 7ml mark while agitating. (Solution will instantly turn milky in color and then clear rapidly by mixing. No precipitate should be formed).4. 2% sodium borate was added to the solution to the 8ml mark, mixed and centrifuged before use.

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Isolation of Antithrombin III from Normal and α1-Antitrypsin-Deficient Human Plasma

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651853 ◽

1977 ◽

Vol 38 (02) ◽

pp. 0475-0485 ◽

Cited By ~ 13

Author(s):

Anna D. Borsodi ◽

Ralph A. Bradshaw

Keyword(s):

Enzymatic Activity ◽

Isoelectric Focusing ◽

Antithrombin Iii ◽

Factor Xa ◽

Antithrombin Activity ◽

Bovine Trypsin ◽

Normal Plasma ◽

Antitrypsin Deficiency ◽

Simplified Procedure ◽

Stimulation Of

SummaryThe plasma of individuals, hetero- or homozygous for α1-antitrypsin deficiency, contains greatly decreased amounts of antithrombin activity as assayed against factor Xa. However, heparin stimulation of the residual antithrombin activity is observed, which is comparable to that of normal plasma. Antithrombins isolated from both normal and α1-antitrypsin deficient plasma by a simplified procedure are indistinguishable in both properties and yields. The microheterogeneity observed on isoelectric focusing of both preparations can be eliminated by treatment with neuraminidase. Neither purified human antithrombin nor α1-antitrypsin, when assayed against bovine trypsin, is stimulated by heparin. These results clearly establish the unique natures of antithrombin and α1-antitrypsin and show that about 75% of the antithrombin activity measured in normal plasma is due to α1-antitrypsin. Estimates of anti thrombin III activity in normal plasma by assays dependent on enzymatic activity can probably be obtained only in the presence of heparin.

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