scholarly journals Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system

2022 ◽  
Vol 7 (1) ◽  
pp. 522-532
Author(s):  
Jiaqi Hou ◽  
Xinjie Chen ◽  
Nan Jiang ◽  
Yanan Wang ◽  
Yi Cui ◽  
...  
Author(s):  
Shijie Ye ◽  
Allison Ann Berger ◽  
Dominique Petzold ◽  
Oliver Reimann ◽  
Benjamin Matt ◽  
...  

This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of discrete fluorines’ interaction with canonical amino acids in a native protein environment and to enable the design of fluorinated proteins with arbitrary desired properties.


ChemBioChem ◽  
2007 ◽  
Vol 8 (14) ◽  
pp. 1650-1653 ◽  
Author(s):  
Norihito Muranaka ◽  
Masanori Miura ◽  
Hikaru Taira ◽  
Takahiro Hohsaka

2012 ◽  
Vol 51 (9) ◽  
pp. 2243-2246 ◽  
Author(s):  
Karin V. Loscha ◽  
Anthony J. Herlt ◽  
Ruhu Qi ◽  
Thomas Huber ◽  
Kiyoshi Ozawa ◽  
...  

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