Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis
Keyword(s):
A Cell
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This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of discrete fluorines’ interaction with canonical amino acids in a native protein environment and to enable the design of fluorinated proteins with arbitrary desired properties.
1993 ◽
Vol 13
(6)
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pp. 3340-3349
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Keyword(s):
2007 ◽
Vol 361
(3)
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pp. 794-799
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Keyword(s):
2011 ◽
pp. 229-239
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1993 ◽
Vol 13
(6)
◽
pp. 3340-3349
Keyword(s):