DNA integrity of canine spermatozoa during chill storage assessed by the sperm chromatin dispersion test using bright-field or fluorescence microscopy

2015 ◽  
Vol 84 (3) ◽  
pp. 399-406 ◽  
Author(s):  
M. Hidalgo ◽  
M. Urbano ◽  
I. Ortiz ◽  
S. Demyda-Peyras ◽  
M.R. Murabito ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Bright Field ◽  
Chill Storage ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

Sperm selection by swim-up in terms of deoxyribonucleic acid fragmentation as measured by the sperm chromatin dispersion test is altered in heavy smokers

2007 ◽  
Vol 88 (2) ◽  
pp. 523-525 ◽  
Author(s):  
Thamara Viloria ◽  
Nicolás Garrido ◽  
José Luis Fernández ◽  
José Remohí ◽  
Antonio Pellicer ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Sperm Chromatin ◽  
Sperm Selection ◽  
Dispersion Test ◽  
Heavy Smokers ◽  
Sperm Chromatin Dispersion Test

Evaluation of stallion sperm DNA during cryopreservation using the Toluidine Blue stain and the Sperm Chromatin Dispersion test

2012 ◽  
Vol 32 (8) ◽  
pp. 480 ◽  
Author(s):  
M.I. Carretero ◽  
C.C. Arraztoa ◽  
A. Ferrante ◽  
M. Caldevilla ◽  
R. Santa Cruz ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Sperm Chromatin ◽  
Sperm Dna ◽  
Blue Stain ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

Sperm chromatin dispersion test (SCDt) for the assessment of sperm DNA fragmentation in black tiger prawn, Penaeus monodon

Aquaculture ◽  
2018 ◽  
Vol 491 ◽  
pp. 281-288 ◽  
Author(s):  
Tianyi Feng ◽  
Jamie Gosálvez ◽  
Carmen Lopez-Fernandez ◽  
Francisca Arroyo ◽  
Brian Paterson ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Penaeus Monodon ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Black Tiger Prawn ◽  
Tiger Prawn ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

Evaluation of DNA fragmentation in llama (Lama glama) sperm using the sperm chromatin dispersion test

2012 ◽  
Vol 131 (1-2) ◽  
pp. 63-71 ◽  
Author(s):  
M.I. Carretero ◽  
D. Lombardo ◽  
C.C. Arraztoa ◽  
S.M. Giuliano ◽  
M.C. Gambarotta ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Dispersion Test ◽  
Lama Glama ◽  
Sperm Chromatin Dispersion Test

Assessment of Sperm DNA Fragmentation in Stallion (Equus caballus) and Donkey (Equus asinus) Using the Sperm Chromatin Dispersion Test

2009 ◽  
Vol 44 (5) ◽  
pp. 823-828 ◽  
Author(s):  
EI Cortés-Gutiérrez ◽  
F Crespo ◽  
C Serres-Dalmau ◽  
AL Gutiérrez de las Rozas ◽  
MI Dávila-Rodríguez ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Equus Caballus ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Equus Asinus ◽  
Sperm Dna ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

scholarly journals In Vitro Studying the Effect of Adding Autologous Platelet Rich Plasma (PRP) to the Human Semen on the Sperm DNA Integrity

2021 ◽  
Vol 10 (2) ◽  
pp. 90-100
Author(s):  
Dhafer Hamdan ◽  
Ali Rahim ◽  
Ula Al-Kawaz
Keyword(s):  
Dna Fragmentation ◽  
Assisted Reproductive Technologies ◽  
Platelet Rich Plasma ◽  
Reproductive Technologies ◽  
Human Reproduction ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Dispersion Test

For conception and the development of healthy embryos, sperm DNA integrity is crucial. According to a growing body of studies, there is a strong correlation between sperm DNA damage and male infertility. Among the new medicines being developed in the medical field, the application of Platelet Rich Plasma (PRP) in human reproduction has yet to be examined. A total of 100 semen samples were used in the current experimental investigation. From November 2020 to June 2021, the research was conducted at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies. Masturbation was used to get an ejaculated semen sample. After semen analysis, the samples were separated into two equal parts, one without autologous PRP and the other with 2% autologous PRP, with the DNA fragmentation assessed using the Sperm Chromatin Dispersion Test. There was highly significant reduction in DNA fragmentation index (p < 0.001). The mean sperm DNA integrity was reduced after adding PRP (33.85±16.73 vs 38.55±16.64), Mean (± SE). PRP has been shown to improve human sperm DNA integrity.

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