Sample stability for routine coagulation testing

2020 ◽  
Vol 196 ◽  
pp. 130-134
Author(s):  
Emmanuel J. Favaloro ◽  
Soma Mohammed ◽  
Giuseppe Lippi
Keyword(s):  
Sample Stability ◽  
Coagulation Testing ◽  
Routine Coagulation

scholarly journals Influence of Centrifuge Temperature on Routine Coagulation Testing

2006 ◽  
Vol 52 (3) ◽  
pp. 537-538 ◽  
Author(s):  
Giuseppe Lippi ◽  
Gian Luca Salvagno ◽  
Martina Montagnana ◽  
Giovanni Poli ◽  
Gian Cesare Guidi
Keyword(s):  
Coagulation Testing ◽  
Routine Coagulation

Minimum citrate tube fill volume for routine coagulation testing

2013 ◽  
Vol 36 (4) ◽  
pp. 493-495 ◽  
Author(s):  
L. Pretorius ◽  
W. J. Janse van Rensburg ◽  
C. Conradie ◽  
M. J. Coetzee
Keyword(s):  
Coagulation Testing ◽  
Fill Volume ◽  
Routine Coagulation

scholarly journals Preanalytical Variables in Coagulation Testing: Setting the Stage for Accurate Results

2019 ◽  
Vol 45 (05) ◽  
pp. 433-448 ◽  
Author(s):  
Robert C. Gosselin ◽  
Richard A. Marlar
Keyword(s):  
Laboratory Practice ◽  
Coagulation Assays ◽  
Practice Recommendations ◽  
Specimen Collection ◽  
Coagulation Testing ◽  
Specimen Processing ◽  
Processing And Storage ◽  
And Storage ◽  
Routine Coagulation

AbstractMany preanalytical variables may affect the results of routine coagulation assays. While advances in laboratory instrumentation have partially addressed the laboratory's ability to recognize some of these variables, there remains an increased reliance on laboratory personnel to recognize the three potential areas where coagulation testing preanalytical issues may arise: (1) specimen collection (including patient selection), (2) specimen transportation and stability, and (3) specimen processing and storage. The purpose of this article is to identify the preanalytical variables associated with coagulation-related testing and provide laboratory practice recommendations in an effort to improve the quality of coagulation testing and accuracy of result reporting.

Influence of the needle bore size on platelet count and routine coagulation testing

2006 ◽  
Vol 17 (7) ◽  
pp. 557-561 ◽  
Author(s):  
Giuseppe Lippi ◽  
Gian Luca Salvagno ◽  
Martina Montagnana ◽  
Giovanni Poli ◽  
Gian Cesare Guidi
Keyword(s):  
Platelet Count ◽  
Coagulation Testing ◽  
Routine Coagulation

scholarly journals Characteristics of routine coagulationtesting and thromboelastometry in polytrauma patients at admission

2021 ◽  
Vol 63 (9) ◽  
pp. 1-5
Author(s):  
Thi Hang Tran ◽  
◽  
Thi Thu Hien Trinh ◽  
Van Chinh Nguyen ◽  
An Son Doan ◽  
...  
Keyword(s):  
University Hospital ◽  
Clot Lysis ◽  
Whole Blood Assay ◽  
Coagulation Parameters ◽  
Blood Assay ◽  
Coagulation Testing ◽  
Coagulation Tests ◽  
Polytrauma Patients ◽  
Old Men ◽  
Routine Coagulation

Background: polytrauma is one of the emergency surgeries with a high mortality rate. One of the leading causes of death is coagulopathy that is not detected early and treated promptly. Thromboelastometry (ROTEM) is a whole blood assay that evaluates the viscoelastic properties during clot formation and clot lysis. This method can detect coagulopathy rapidly and accurately, thereby improving the management of bleeding after trauma. Objectives: describing and evaluating the correlation between ROTEM parameters and routine coagulation tests in polytrauma patients at admission. Method: 110 patients admitted to the Emergency Department, Viet Duc University Hospital from May 2021 to July 2021 were diagnosed with polytrauma. All patients underwent routine coagulation testing and ROTEM parameters at admission. Result: the average age of patients is 41.4±14.7 years old, men accounts for 77.3%, average ISS score is 24.5±6.3. The proportion of the polytrauma patients with coagulopathy by routine coagulation testing was 50.9%. A significant correlation was found between routine coagulation parameters and ROTEM: between APTT with CFT-INTEM (r=0.65; p<0.01); between PT and CFT-EXTEM (r=0.64; p<0.01); between platelet count and MCF-INTEM (r=0.56; p=0.00) and MCF-EXTEM (r=0.57; p=0.00); between fibrinogen level and MCF-INTEM (r=0.71; p=0.00), MCF-EXTEM (r=0.71; p=0.00), and MCF-FIBTEM (r=0.91; p=0.00). Conclusion: proportion of the polytrauma patients with coagulopathy by routine coagulation testing was 50.9%. A significant correlation was found between routine coagulation parameters and ROTEM

scholarly journals The effects of fructose diphosphate on routine coagulation tests in vitro

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Tongqing Chen ◽  
Duan Chen ◽  
Lu Chen ◽  
Zhengxu Chen ◽  
Baolong Wang ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Blood Coagulation ◽  
Detection System ◽  
Coagulation System ◽  
Thrombin Time ◽  
Aggregation Rate ◽  
Coagulation Testing ◽  
Coagulation Tests ◽  
Routine Coagulation

AbstractTo evaluate the effects of fructose diphosphate (FDP) on routine coagulation tests in vitro, we added FDP into the mixed normal plasma to obtain the final concentration of 0, 1, 2, 3, 4, 5, 6, 10, 15, 20, 25, 30 and 35 mg/mL of drug. Prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen (FBG) and thrombin time (TT) of samples were analyzed with blood coagulation analyzers from four different manufacturers(Sysmex, Stago, SEKISUI and Werfen) and their corresponding reagents, respectively. Before the experiment, we also observed whether there were significant differences in coagulation test results of different lots of reagents produced by each manufacturer. At the same time as the four routine clotting tests, the Sysmex blood coagulation analyzer and its proprietary analysis software were used to detect the change of maximum platelet aggregation rate in platelet-rich plasma after adding FDP (0, 1, 2, 3, 4, 5 and 6 mg/mL). The results of PT, aPTT and TT showed a FDP (0–35 mg/mL) concentration-dependent increase and a FBG concentration-dependent decrease. The degree of change (increase or decrease) varied depending on the assay system, with PT and aPTT being more affected by the Sysmex blood coagulation testing instrument reagent system and less affected by CEKISUI, TT less affected by CEKISUI and more affected by Stago, and FBG less affected by Stago and more affected by Sysmex. The results of PT, aPTT and TT were statistically positively correlated with their FDP concentrations, while FBG was negatively correlated. The correlation coefficients between FDP and the coagulation testing systems of Sysmex, Stago, Werfen and SEKISUI were 0.975, 0.988, 0.967, 0.986 for PT, and 0.993, 0.989, 0.990 and 0.962 for aPTT, 0.994, 0.960, 0.977 and 0.982 for TT, − 0.990, − 0.983, − 0.989 and − 0.954 for FBG, respectively. Different concentrations of FDP (0, 1, 2, 3, 4, 5 and 6 mg/mL) had different effects on the maximum aggregation rate of platelet induced by the agonists of adenosine diphosphate (ADP, 5 µmol/L), arachidonic acid (Ara, 1 mmol/L), collagen (Col, 2.5 µg/mL) and epinephrine (Epi,10 µmol/L), but the overall downward trend was consistent, that is, with the increase of FDP concentration, the platelet aggregation rate decreased significantly. Our experimental study demonstrated a possible effect of FDP on the assays of coagulation and Platelet aggregation, which may arise because the drug interferes with the coagulation and platelet aggregation detection system, or it may affect our in vivo coagulation system and Platelet aggregation function, the real mechanism of which remains to be further verified and studied.

Automation of Routine Coagulation Testing Using a Random Access Centrifugal Analyzer

1988 ◽  
Vol 89 (3) ◽  
pp. 347-352 ◽  
Author(s):  
John A. Rowell ◽  
Mervyn J. Elms ◽  
Joanne Young ◽  
Ian H. Bunce
Keyword(s):  
Random Access ◽  
Coagulation Testing ◽  
Routine Coagulation

Interference of lipemia in samples for routine coagulation testing using a Sysmex CS‐5100 coagulometer

2019 ◽  
Vol 41 (6) ◽  
pp. 772-777
Author(s):  
Davide Negrini ◽  
Daniela Bernardi ◽  
Giorgia Antonelli ◽  
Mario Plebani
Keyword(s):  
Coagulation Testing ◽  
Routine Coagulation

scholarly journals Influence of dabigatran and rivaroxaban on routine coagulation assays

2015 ◽  
Vol 113 (01) ◽  
pp. 154-164 ◽  
Author(s):  
Els Bailleul ◽  
Bernard Chatelain ◽  
Anne Demulder ◽  
Katrien Devreese ◽  
Jonathan Douxfils ◽  
...  
Keyword(s):  
External Quality Assessment ◽  
Nationwide Survey ◽  
Activated Partial Thromboplastin Time ◽  
Dependent Manner ◽  
Activity Assay ◽  
External Quality ◽  
External Quality Assessment Scheme ◽  
Coagulation Assays ◽  
Coagulation Testing ◽  
Routine Coagulation

SummaryThe Belgian national External Quality Assessment Scheme performed a nationwide survey using lyophilised plasma samples spiked with dabigatran or rivaroxaban to demonstrate to the Belgian clinical laboratories how these drugs affect their routine coagulation assays prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen and antithrombin. Virtually all Belgian laboratories performing routine coagulation testing (189/192) participated in the survey. Both, dabigatran and rivaroxaban significantly prolonged the PT and aPTT in a concentration- and reagent-dependent manner. PT reagents were more influenced by rivaroxaban than by dabigatran and aPTT reagents more influenced by dabigatran than by rivaroxaban. Among PT reagents, Neoplastin R® was the most sensitive to rivaroxaban and Innovin ® and Thromborel S® the least sensitive. Converting PT results to INR only increased the variability between reagents. Among aPTT reagents, Actin FSL® was the least sensitive to dabigatran while the other aPTT reagents showed slightly higher sensitivities. The presence of dabigatran led to falsely reduced fibrinogen concentrations when measured with a low thrombin concentration reagent. The presence of dabigatran caused an overestimation of the antithrombin level when measured with a thrombin-based activity assay and the presence of rivaroxaban an overestimation of the antithrombin level when measured with a FXa-based assay. Instrument-related differences were found for all tested parameters. In conclusion, this paper provides detailed information on the effect of dabigatran and rivaroxaban on routine coagulation assays as performed with a large number of reagent/instrument combinations.

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