PHEX neutralizing agent inhibits dentin formation in mouse tooth germ

2011 ◽  
Vol 43 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Hongbing Lv ◽  
Sheng Fu ◽  
Gancha Wu ◽  
Fuhua Yan
Keyword(s):  
Tooth Germ ◽  
Neutralizing Agent ◽  
Dentin Formation

Local Irradiation of Mouse Tooth Germ Gives Insight into the Direct Effects of Irradiation on Root Development

2021 ◽  
Author(s):  
Yoshiaki Ide ◽  
Taka Nakahara ◽  
Tetsuya Fukada ◽  
Masanori Nasu
Keyword(s):  
Root Development ◽  
Root Formation ◽  
Tooth Germ ◽  
Lead Glass ◽  
Local Irradiation ◽  
Root Sheath ◽  
Calcified Tissue ◽  
Radiation Induced ◽  
Dentin Formation ◽  
Root Apices

To elucidate the mechanism underlying the failure of root formation after irradiation, we established a method of local irradiation of the molar tooth germ and demonstrated that radiation directly affected dental root development. In the current study, to locally irradiate the lower first molars of 5-day-old C57BL/6J mice, we used lead glass containing a hole as a collimator. We confirmed that our local irradiation method targeted only the tooth germ. The irradiated root was immature in terms of apical growth, and dentin formation was irregular along the outside of the root apices. Moreover, calcified tissue apically surrounded Hertwig's epithelial root sheath, which disappeared abnormally early. This method using a local irradiation experimental model will facilitate research into radiation-induced disorders of dental root formation.

Expression and localization of CRAMP in rat tooth germ and during reparative dentin formation

2018 ◽  
Vol 22 (7) ◽  
pp. 2559-2566 ◽  
Author(s):  
Kanji Horibe ◽  
Akihiro Hosoya ◽  
Toru Hiraga ◽  
Hiroaki Nakamura
Keyword(s):  
Tooth Germ ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

Autoradiography of Incorporated Leucine-H3 in the Enamel Matrix and Enamel Organ of the Upper Incisor of the Rat

1964 ◽  
Vol 04 (02) ◽  
pp. 186-192
Author(s):  
Leonel Costacurta
Keyword(s):  
Amino Acid ◽  
Blood Vessels ◽  
Tooth Germ ◽  
Longitudinal Section ◽  
Equal Length ◽  
Enamel Organ ◽  
Enamel Matrix ◽  
Distal Extremity ◽  
Old Rats ◽  
Thin Band

SummaryDental germs of the upper incisors of six-days old rats were studied for the uptake of leucine-H3 by different layers of the enamel organ in correlation to the various stages of the development of enamel.The longitudinal section of the tooth germ was divided into 15 zones of about equal length in order to facilitate the description and interpretation of results. Autoradiographic images of the histologic preparations from rats sacrificed 30 minutes, 1 hour, 1 day and 3 days after the injection were made. The strongest reactions were observed in dental germs of rats sacrificed 1 hour, and particularly one day, after the leucine-H3 injection.The uptake of this compound by the enamel matrix increases progressively up to the young enamel and then decreases to the distal extremity; the greatest quantity of this labeled amino-acid was observed in the primary and young enamel. The reactions were present in the transitional enamel only along a thin band close to the dentine-enamel junction.In the enamel organ leucine-H3 incorporation was greatest in the three layers, the zones corresponding to primary and young enamel. In zones corresponding to transitional enamel, the inner epithelium showed a small quantity, and the stellate reticulum a blackening only in its superficial part, were the blood vessels reach the enamel organ.

Effect of a Calcium-silicate-based Restorative Cement on Pulp Repair

2012 ◽  
Vol 91 (12) ◽  
pp. 1166-1171 ◽  
Author(s):  
X.V. Tran ◽  
C. Gorin ◽  
C. Willig ◽  
B. Baroukh ◽  
B. Pellat ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Glass Ionomer Cement ◽  
Repair Process ◽  
Male Rats ◽  
Time Points ◽  
Tertiary Dentin ◽  
Pulp Exposure ◽  
Calcium Silicate Cements ◽  
Reparative Process ◽  
Dentin Formation

In cases of pulp injury, capping materials are used to enhance tertiary dentin formation; Ca(OH)2 and MTA are the current gold standards. The aim of this study was to evaluate the capacity of a new calcium-silicate-based restorative cement to induce pulp healing in a rat pulp injury model. For that purpose, cavities with mechanical pulp exposure were prepared on maxillary first molars of 27 six-week-old male rats, and damaged pulps were capped with either the new calcium-silicate-based restorative cement (Biodentine), MTA, or Ca(OH)2. Cavities were sealed with glass-ionomer cement, and the repair process was assessed at several time-points. At day 7, our results showed that both the evaluated cement and MTA induced cell proliferation and formation of mineralization foci, which were strongly positive for osteopontin. At longer time-points, we observed the formation of a homogeneous dentin bridge at the injury site, secreted by cells displaying an odontoblastic phenotype. In contrast, the reparative tissue induced by Ca(OH)2 showed porous organization, suggesting a reparative process different from those induced by calcium silicate cements. Analysis of these data suggests that the evaluated cement can be used for direct pulp-capping.

scholarly journals Effects of temperature and neutralizing agent on lactic acid production by Rhizopus sp. fermentation

2021 ◽  
Author(s):  
Whiny Erliana ◽  
Tri Widjaja ◽  
Tectona Indah ◽  
Daryl Susilo ◽  
Annisa Dewi
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Neutralizing Agent ◽  
Effects Of Temperature ◽  
Rhizopus Sp

In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells

2014 ◽  
Vol 39 (1) ◽  
pp. 94-103 ◽  
Author(s):  
Ayşegül Doğan ◽  
Selami Demirci ◽  
Fikrettin Şahin
Keyword(s):  
Stem Cells ◽  
Tooth Germ ◽  
Human Tooth ◽  
In Vitro Differentiation ◽  
Human Tooth Germ

The application of zinc calcine as a neutralizing agent for the goethite process in zinc hydrometallurgy

2014 ◽  
Vol 147-148 ◽  
pp. 120-126 ◽  
Author(s):  
Haisheng Han ◽  
Wei Sun ◽  
Yuehua Hu ◽  
Honghu Tang
Keyword(s):  
Neutralizing Agent ◽  
Zinc Hydrometallurgy

scholarly journals Involvement of the Klotho Protein in Dentin Formation and Mineralization

2008 ◽  
Vol 291 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Hironobu Suzuki ◽  
Norio Amizuka ◽  
Kimimitsu Oda ◽  
Masaki Noda ◽  
Hayato Ohshima ◽  
...  
Keyword(s):  
Klotho Protein ◽  
Dentin Formation

scholarly journals BMP Activity Is Required for Tooth Development from the Lamina to Bud Stage

2012 ◽  
Vol 91 (7) ◽  
pp. 690-695 ◽  
Author(s):  
Y. Wang ◽  
L. Li ◽  
Y. Zheng ◽  
G. Yuan ◽  
G. Yang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Molecular Marker ◽  
Tooth Development ◽  
Tooth Germ ◽  
Bmp Signaling ◽  
Expression Of Genes ◽  
Dental Epithelium ◽  
Initiation Stage ◽  
Transgenic Embryos ◽  
Bmp Antagonist

Several Bmp genes are expressed in the developing mouse tooth germ from the initiation to the late-differentiation stages, and play pivotal roles in multiple steps of tooth development. In this study, we investigated the requirement of BMP activity in early tooth development by transgenic overexpression of the extracellular BMP antagonist Noggin. We show that overexpression of Noggin in the dental epithelium at the tooth initiation stage arrests tooth development at the lamina/early-bud stage. This phenotype is coupled with a significantly reduced level of cell proliferation rate and a down-regulation of Cyclin-D1 expression, specifically in the dental epithelium. Despite unaltered expression of genes known to be implicated in early tooth development in the dental mesenchyme and dental epithelium of transgenic embryos, the expression of Pitx2, a molecular marker for the dental epithelium, became down-regulated, suggesting the loss of odontogenic fate in the transgenic dental epithelium. Our results reveal a novel role for BMP signaling in the progression of tooth development from the lamina stage to the bud stage by regulating cell proliferation and by maintaining odontogenic fate of the dental epithelium.

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