Effect of a Calcium-silicate-based Restorative Cement on Pulp Repair

2012 ◽  
Vol 91 (12) ◽  
pp. 1166-1171 ◽  
Author(s):  
X.V. Tran ◽  
C. Gorin ◽  
C. Willig ◽  
B. Baroukh ◽  
B. Pellat ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Glass Ionomer Cement ◽  
Repair Process ◽  
Male Rats ◽  
Time Points ◽  
Tertiary Dentin ◽  
Pulp Exposure ◽  
Calcium Silicate Cements ◽  
Reparative Process ◽  
Dentin Formation

In cases of pulp injury, capping materials are used to enhance tertiary dentin formation; Ca(OH)2 and MTA are the current gold standards. The aim of this study was to evaluate the capacity of a new calcium-silicate-based restorative cement to induce pulp healing in a rat pulp injury model. For that purpose, cavities with mechanical pulp exposure were prepared on maxillary first molars of 27 six-week-old male rats, and damaged pulps were capped with either the new calcium-silicate-based restorative cement (Biodentine), MTA, or Ca(OH)2. Cavities were sealed with glass-ionomer cement, and the repair process was assessed at several time-points. At day 7, our results showed that both the evaluated cement and MTA induced cell proliferation and formation of mineralization foci, which were strongly positive for osteopontin. At longer time-points, we observed the formation of a homogeneous dentin bridge at the injury site, secreted by cells displaying an odontoblastic phenotype. In contrast, the reparative tissue induced by Ca(OH)2 showed porous organization, suggesting a reparative process different from those induced by calcium silicate cements. Analysis of these data suggests that the evaluated cement can be used for direct pulp-capping.

scholarly journals Surface Pre-Reacted Glass Filler Contributes to Tertiary Dentin Formation through a Mechanism Different Than That of Hydraulic Calcium-Silicate Cement

2019 ◽  
Vol 8 (9) ◽  
pp. 1440 ◽  
Author(s):  
Motoki Okamoto ◽  
Manahil Ali ◽  
Shungo Komichi ◽  
Masakatsu Watanabe ◽  
Hailing Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Calcium Silicate ◽  
Dental Pulp ◽  
Healing Process ◽  
Calcium Silicate Cement ◽  
Tertiary Dentin ◽  
Regulated Gene Expression ◽  
Dentin Formation ◽  
Hydraulic Calcium Silicate Cement

The induction of tissue mineralization and the mechanism by which surface pre-reacted glass-ionomer (S-PRG) cement influences pulpal healing remain unclear. We evaluated S-PRG cement-induced tertiary dentin formation in vivo, and its effect on the pulp cell healing process in vitro. Induced tertiary dentin formation was evaluated with micro-computed tomography (μCT) and scanning electron microscopy (SEM). The distribution of elements from the S-PRG cement in pulpal tissue was confirmed by micro-X-ray fluorescence (μXRF). The effects of S-PRG cement on cytotoxicity, proliferation, formation of mineralized nodules, and gene expression in human dental pulp stem cells (hDPSCs) were assessed in vitro. μCT and SEM revealed that S-PRG induced tertiary dentin formation with similar characteristics to that induced by hydraulic calcium-silicate cement (ProRoot mineral trioxide aggregate (MTA)). μXRF showed Sr and Si ion transfer into pulpal tissue from S-PRG cement. Notably, S-PRG cement and MTA showed similar biocompatibility. A co-culture of hDPSCs and S-PRG discs promoted mineralized nodule formation on surrounding cells. Additionally, S-PRG cement regulated the expression of genes related to osteo/dentinogenic differentiation. MTA and S-PRG regulated gene expression in hDPSCs, but the patterns of regulation differed. S-PRG cement upregulated CXCL-12 and TGF-β1 gene expression. These findings showed that S-PRG and MTA exhibit similar effects on dental pulp through different mechanisms.

Interfacial Properties of Three Different Bioactive Dentine Substitutes

2013 ◽  
Vol 19 (6) ◽  
pp. 1450-1457 ◽  
Author(s):  
Elizabeta S. Gjorgievska ◽  
John W. Nicholson ◽  
Sonja M. Apostolska ◽  
Nichola J. Coleman ◽  
Samantha E. Booth ◽  
...  
Keyword(s):  
Particle Size ◽  
Ion Exchange ◽  
Calcium Silicate ◽  
Artificial Saliva ◽  
Glass Ionomer Cement ◽  
Longitudinal Axis ◽  
Glass Ionomer ◽  
Bioactive Materials ◽  
Calcium Silicate Cements ◽  
Tooth Repair

AbstractThree different bioactive materials suitable as dentine substitutes in tooth repair have been studied: glass-ionomer cement, particulate bioglass, and calcium-silicate cement. On 15 permanent human molars, Class V cavities were prepared and the bottom of each cavity was de-mineralized by an artificial caries gel. After the de-mineralization, the teeth were restored with: (1) Bioglass®45S5 and ChemFil® Superior; (2) Biodentine™ and ChemFil® Superior; and (3) ChemFil® Superior for a complete repair. The teeth were stored for 6 weeks in artificial saliva, then cut in half along the longitudinal axis: the first half was imaged in a scanning electron microscope (SEM) and the other half was embedded in resin and analyzed by SEM using energy-dispersive X-ray analysis. The glass-ionomer and the bioglass underwent ion exchange with the surrounding tooth tissue, confirming their bioactivity. However, the particle size of the bioglass meant that cavity adaptation was poor. It is concluded that smaller particle size bioglasses may give more acceptable results. In contrast, both the glass-ionomer and the calcium-silicate cements performed well as dentine substitutes. The glass-ionomer showed ion exchange properties, whereas the calcium silicate gave an excellent seal resulting from its micromechanical attachment.

scholarly journals Solubility and porosity of new nanostructured calcium silicate cement

2014 ◽  
Vol 61 (4) ◽  
pp. 190-195
Author(s):  
Tatjana Stanic ◽  
Violeta Pavlovic ◽  
Vukoman Jokanovic ◽  
Marija Zivkovic-Sandic ◽  
Slavoljub Zivkovic
Keyword(s):  
Physical Properties ◽  
Calcium Silicate ◽  
Glass Ionomer Cement ◽  
Glass Ionomer ◽  
Cement Material ◽  
Calcium Silicate Cement ◽  
Metal Rings ◽  
Calcium Silicate Cements ◽  
Plastic Containers ◽  
Ionomer Cement

Introduction. Calcium silicate cements are most commonly used materials in endodontics for many indications due to their exceptional biological and physical properties. The aim of this study was to assess solubility and porosity of new experimental nanostructured calcium silicate cement. Material and Methods. A novel nanostructured calcium silicate cement (CS), commercialized calcium silicate cement (Biodentin), traditional glass ionomer cement (GIC) (Micron Superior) and resin-reinforced glass ionomer cement (Fuji VIII) were used in this study. All materials were prepared as per manufactuers? instructions, placed in metal rings of 16?2 mm diameter and kept in an incubator at 37?C for 24 hours. After that they were weighed and put in plastic containers filled with 25 ml of water for additional 24 hours. Then after all samples were weighed again and resorption and solubility were calculated. Results. The highest solubility was found for new CS cement (12.45) followed by conventional GIC Micron Superior (11.5) and Biodentin (6.1) whereas the lowest solubility was for resin reinforced GIC Fuji VIII (3.8). These differences were statistically significant (p<0.005). The highest absorption was also observed in new CS cement (24.15), followed by Biodentin (18.5) and Micron superior (17.95) while the lowest was for GIC Fuji VIII (7.75). These differences were also statistically significant except between Micron Superior and Biodentin. Conclusion. Solubility and porosity of new nanostructured calcium silicate cement were significantly higher than for traditional calcium silicate cement, traditional GIC and resin-reinforced GIC.

The aqueous tuber extract of Pueraria tuberosa (Willd.) D.C. caused cytotoxic effect on HT 29 cell lines with down regulation of nuclear factor-kappa B (NF-κB)

2017 ◽  
Vol 16 (4) ◽  
Author(s):  
Adeolu Alex Adedapo ◽  
Olusegun A Fagbohun ◽  
Christianah Dawurung ◽  
Ademola Adetokunbo Oyagbemi ◽  
Temidayo Olutayo Omobowale ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cytotoxic Effect ◽  
Nuclear Factor Κb ◽  
Male Rats ◽  
Root Extract ◽  
Nuclear Factor ◽  
Down Regulation ◽  
Time Points ◽  
Pueraria Tuberosa ◽  
Ht 29

Abstract Background Pueraria tuberosa (Willd) D.C. (Fabaceae) tubers are already used in traditional medicine by Ayurvedic physicians for the management of fertility disorders, general weakness, and also as anti-ageing therapies. Other known pharmacological properties include: anti-hyperglycemics, hepatoprotective, anti-hyperlipidemic, diuretic, nutritive, and anti-fertility agents in male rats. Methods The anti-proliferative effect of the aqueous tuberous root extract of Pueraria tuberosa on vascular smooth muscle cells (VSMCs) and Human Colorectal Adenocarcinoma Cell lines (HT-29) was investigated using the Cell Titer 96 MTT Proliferation Assay where the viable cells were seeded at a density of 5 × 104 (100 µL/well). For VSMC, log concentrations of the extract at 200 and 800 µg/mL were added and incubated for 24 and 48 h time points. Incubation of the extract in the presence of vascular endothelial growth factor (VEGF) and ET-1 was also conducted at different times. Concentrations of the extract (200, 400 and 700 µg/mL) were also added and incubated with the HT 29 cell lines for 24, 48 and 72 h time points. The effect of the tuber aqueous extract of the plant on nuclear factor-κB (NF-κB) expression after 2 h was also carried out using immunoblotting technique. Results The result showed that after 24 h, the effect of the extract in the presence of the mitogens and on the VSMC was more of proliferation. However, at 48 h, the 200 µg/mL dose, both alone and in the presence of VEGF caused 11.1% and 25.9% decreases respectively, in cell proliferation. In the HT 29 cytotoxic study the 200 µg/mL concentration caused the greatest cytotoxic effect at 77.1% cell inhibition followed by 400 µg/mL concentration at 71.4% after 72 h. The immunoblotting assay showed a down regulation of NF-κB expressions with 0.7 µg/mL concentration showing the greatest effect. NF-κB, a pro-inflammatory agent is increasingly recognized as a crucial player in many steps of cancer initiation and progression. Conclusions It could therefore be concluded that the aqueous root extract of Pueraria tuberosa possesses cytotoxic effect and could serve as a lead compound for anticancer and anti-inflammatory agents.

scholarly journals Does Delayed Restoration Improve Shear Bond Strength of Different Restorative Protocols to Calcium Silicate-Based Cements?

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2216 ◽  
Author(s):  
Paulo Palma ◽  
Joana Marques ◽  
Rui Falacho ◽  
Alexandra Vinagre ◽  
João Santos ◽  
...  
Keyword(s):  
Bond Strength ◽  
Calcium Silicate ◽  
Shear Bond Strength ◽  
Proper Time ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
Mineral Trioxide Aggregate ◽  
Bonding System ◽  
Post Hoc ◽  
Ionomer Cement

The purpose of the present study was to assess the proper time to perform a restoration (immediately or delayed) after placement of two calcium silicate-based cements (CSCs) and to test the performance of two different restorative protocols regarding shear bond strength (SBS). Seventy-five acrylic blocks were randomly divided into five groups (n = 15). Specimens were filled with either ProRoot MTA (Dentsply Tulsa Dental) or Biodentine (Septodont). The restoration was performed at an immediate (12 min) or delayed (seven days) timeframe, using a resin-based flowable composite (SDR) (bonded to the CSC using a universal bonding system) or glass ionomer cement (GIC) as restorative materials. SBS was measured using a universal testing machine. Fractured surfaces were evaluated, and the pattern was registered. Statistical analysis was performed using the Dunn–Sidak post hoc test (P < 0.05). Biodentine/immediate SDR showed the highest mean SBS value (4.44 MPa), with statistically significant differences when compared to mineral trioxide aggregate (MTA)/GIC (1.14 MPa) and MTA/immediate SDR (1.33 MPa). MTA/GIC and MTA/immediate SDR did not present significant differences regarding SBS. No statistical differences were verified concerning mean SBS between both CSCs within the 7 day groups. MTA/delayed SDR (3.86 MPa) presented statistical differences compared to MTA/immediate SDR, whereas no differences were observed regarding Biodentine performance (Biodentine/immediate SDR and Biodentine/delayed SDR (3.09 MPa)). Bonding procedures directly on top of MTA might be preferably performed at a delayed timeframe, whereas Biodentine might allow for immediate restoration.

scholarly journals Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite

2016 ◽  
Vol 63 (4) ◽  
pp. 183-192 ◽  
Author(s):  
Marijana Popović Bajić ◽  
Violeta Petrović ◽  
Vanja Opačić Galić ◽  
Vesna Danilović ◽  
Vukoman Jokanović ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Healing Process ◽  
Glass Ionomer Cement ◽  
Control Group ◽  
Therapeutic Effects ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Nanostructural Material ◽  
Experimental Group

Summary Introduction Direct pulp capping is an important therapeutic method that has goal to provide formation of dentin bridge and healing process of the pulp. The aim of this study was to investigate the effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite on exposed dental pulp in Vietnamese pigs. Material and Methods The study was conducted on 30 teeth of two Vietnamese pigs (Sus scrofa verus). On buccal surfaces of incisors, canines and first premolars, class V cavities were prepared with a small round bur and pulp horn was exposed. In the first experimental group (10 teeth) the perforation was covered with new nanostructural material based on calcium silicate systems (CS). In the second experimental group, the perforation was covered with compound of calcium silicate systems and hydroxyapatite (HA-CS) (10 teeth). In the control group, exposed pulp was covered with Pro Root MTA® (Dentsply Tulsa Dental, Johnson City, TN, USA) (10 teeth). All cavities were restored with glass ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). Observation period was 28 days. After sacrificing the animals, histological preparations were done to analyze the presence of dentin bridge, inflammatory reaction of the pulp, pulp tissue reorganization and the presence of bacteria. Results Dentin bridge was observed in all teeth (experimental and control groups). Inflammation of the pulp was mild to moderate in all groups. Neoangiogenesis and many odontoblast like cells responsible for dentin bridge formation were detected. Necrosis was not observed in any case, neither the presence of Gram-positive bacteria in the pulp. Conclusion Histological analysis indicated favorable therapeutic effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite for direct pulp capping in teeth of Vietnamese pigs.

scholarly journals Dynamics of the Activity of MAP-Kinase Cascades in the Healing Process of Postoperative Musculocutaneous Wounds

2019 ◽  
Vol 4 (5) ◽  
pp. 55-59
Author(s):  
I. A. Shurygina ◽  
V. A. Umanets ◽  
M. G. Shurygin
Keyword(s):  
Map Kinase ◽  
Healing Process ◽  
Traumatic Injury ◽  
Repair Process ◽  
Modern Medicine ◽  
Peak Activity ◽  
Specific Staining ◽  
Mapk Cascades ◽  
Reparative Process ◽  
The Impact

Background. Management of the reparative process is an urgent task of modern medicine. In our opinion, the development of pathogenetically grounded approaches to optimizing the repair process for managing the interrelations of stromal cells is promising. One of the promising areas in this regard is the impact on the MAPK-cascades.Aim: to study the expression of MAP-kinase mechanisms in the regulation of repair by the example of a musculocutaneous wound.Methods. A linear muscular skin wound was modeled using Wistar rats weighing 220–250 g at the age of 9 months (n = 24). Immunofluorescence staining was performed to detect the activity of p38, JNK, and ERK MAPK cascades from 1 to 30 days.Results. It was established that specific staining in the area of connective tissue formation during staining with p38 MAPK and its phosphorylated form was first observed on the 3rd day, and its maximum severity occurred at the same time. On the 7th and 14th day, small zones in the area of scar formation were minimally stained. The phosphorylated part of the JNK-cascade in the zone of traumatic injury was detected starting from the 1st day after the injury. Bright color persisted on the 3rd day. On the 7th day, the color was minimal, and by the 14th day a second wave of expression was observed. ERK-staining was observed from the 1st to the 14th day with a peak activity on the 3rd day.Conclusion. Thus, we revealed the simultaneous involvement of p38, JNK-, and ERK-cascades in the regulation of the reparative process in the conditions of a musculoskeletal wound. At the same time, it is noteworthy that the peak activity of all cascades coincides and falls on the 3rd day.

The use of calcium-silicate cements to reduce dentine permeability

2012 ◽  
Vol 57 (8) ◽  
pp. 1054-1061 ◽  
Author(s):  
M.G. Gandolfi ◽  
F. Iacono ◽  
C. Pirani ◽  
C. Prati
Keyword(s):  
Calcium Silicate ◽  
Calcium Silicate Cements

scholarly journals Quantitative Evaluation by Glucose Diffusion of Microleakage in Aged Calcium Silicate-Based Open-Sandwich Restorations

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
S. Koubi ◽  
H. Elmerini ◽  
G. Koubi ◽  
H. Tassery ◽  
J. Camps
Keyword(s):  
Calcium Silicate ◽  
Glass Ionomer Cement ◽  
Glass Ionomer ◽  
Calcium Silicate Cement ◽  
Resin Modified Glass Ionomer ◽  
Glucose Diffusion ◽  
Negative Controls ◽  
Phosphate Buffered Saline ◽  
Ionomer Cement

This study compared thein vitromarginal integrity of open-sandwich restorations based on aged calcium silicate cement versus resin-modified glass ionomer cement. Class II cavities were prepared on 30 extracted human third molars. These teeth were randomly assigned to two groups () to compare a new hydraulic calcium silicate cement designed for restorative dentistry (Biodentine, Septodont, Saint Maur des Fossés, France) with a resin-modified glass ionomer cement (Ionolux, Voco, Cuxhaven, Germany) in open-sandwich restorations covered with a light-cured composite. Positive () and negative () controls were included. The teeth simultaneously underwent thermocycling and mechanocycling using a fatigue cycling machine (1,440 cycles, 5–55°C; 86,400 cycles, 50 N/cm2). The specimens were then stored in phosphate-buffered saline to simulate aging. After 1 year, the teeth were submitted to glucose diffusion, and the resulting data were analyzed with a nonparametric Mann-Whitney test. The Biodentine group and the Ionolux group presented glucose concentrations of 0.074 ± 0.035 g/L and 0.080 ± 0.032 g/L, respectively. No statistically significant differences were detected between the two groups. Therefore, the calcium silicate-based material performs as well as the resin-modified glass ionomer cement in open-sandwich restorations.

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