scholarly journals Involvement of the Klotho Protein in Dentin Formation and Mineralization

2008 ◽  
Vol 291 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Hironobu Suzuki ◽  
Norio Amizuka ◽  
Kimimitsu Oda ◽  
Masaki Noda ◽  
Hayato Ohshima ◽  
...  
Keyword(s):  
Klotho Protein ◽  
Dentin Formation

Effect of a Calcium-silicate-based Restorative Cement on Pulp Repair

2012 ◽  
Vol 91 (12) ◽  
pp. 1166-1171 ◽  
Author(s):  
X.V. Tran ◽  
C. Gorin ◽  
C. Willig ◽  
B. Baroukh ◽  
B. Pellat ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Glass Ionomer Cement ◽  
Repair Process ◽  
Male Rats ◽  
Time Points ◽  
Tertiary Dentin ◽  
Pulp Exposure ◽  
Calcium Silicate Cements ◽  
Reparative Process ◽  
Dentin Formation

In cases of pulp injury, capping materials are used to enhance tertiary dentin formation; Ca(OH)2 and MTA are the current gold standards. The aim of this study was to evaluate the capacity of a new calcium-silicate-based restorative cement to induce pulp healing in a rat pulp injury model. For that purpose, cavities with mechanical pulp exposure were prepared on maxillary first molars of 27 six-week-old male rats, and damaged pulps were capped with either the new calcium-silicate-based restorative cement (Biodentine), MTA, or Ca(OH)2. Cavities were sealed with glass-ionomer cement, and the repair process was assessed at several time-points. At day 7, our results showed that both the evaluated cement and MTA induced cell proliferation and formation of mineralization foci, which were strongly positive for osteopontin. At longer time-points, we observed the formation of a homogeneous dentin bridge at the injury site, secreted by cells displaying an odontoblastic phenotype. In contrast, the reparative tissue induced by Ca(OH)2 showed porous organization, suggesting a reparative process different from those induced by calcium silicate cements. Analysis of these data suggests that the evaluated cement can be used for direct pulp-capping.

Role of Klotho Protein in Tumor Genesis, Cancer Progression, and Prognosis in Patients with High-Grade Glioma

2019 ◽  
Vol 130 ◽  
pp. e324-e332 ◽  
Author(s):  
Naama Peshes-Yeloz ◽  
Lior Ungar ◽  
Anton Wohl ◽  
Elad Jacoby ◽  
Tamar Fisher ◽  
...  
Keyword(s):  
Cancer Progression ◽  
High Grade Glioma ◽  
High Grade ◽  
Klotho Protein

scholarly journals Antiinflammatory Actions of Klotho: Implications for Therapy of Diabetic Nephropathy

2021 ◽  
Vol 22 (2) ◽  
pp. 956
Author(s):  
Marlena Typiak ◽  
Agnieszka Piwkowska
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Calcium Metabolism ◽  
Renal Involvement ◽  
Therapeutic Effects ◽  
Beneficial Effects ◽  
Early Biomarker ◽  
Klotho Protein ◽  
Urine Levels ◽  
Proinflammatory Factors

Klotho was initially introduced as an antiaging molecule. Klotho deficiency significantly reduces lifespan, and its overexpression extends it and protects against various pathological phenotypes, especially renal disease. It was shown to regulate phosphate and calcium metabolism, protect against oxidative stress, downregulate apoptosis, and have antiinflammatory and antifibrotic properties. The course of diabetes mellitus and diabetic nephropathy resembles premature cellular senescence and causes the activation of various proinflammatory and profibrotic processes. Klotho was shown to exert many beneficial effects in these disorders. The expression of Klotho protein is downregulated in early stages of inflammation and diabetic nephropathy by proinflammatory factors. Therefore, its therapeutic effects are diminished in this disorder. Significantly lower urine levels of Klotho may serve as an early biomarker of renal involvement in diabetes mellitus. Recombinant Klotho administration and Klotho overexpression may have immunotherapeutic potential for the treatment of both diabetes and diabetic nephropathy. Therefore, the current manuscript aims to characterize immunopathologies occurring in diabetes and diabetic nephropathy, and tries to match them with antiinflammatory actions of Klotho. It also gives reasons for Klotho to be used in diagnostics and immunotherapy of these disorders.

scholarly journals Sugar sweetened beverage consumption is positively associated with Klotho levels at two years of age in LatinX youth

BMC Nutrition ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Sofia Villagomez ◽  
Dena B. Dubal ◽  
Jessica Hawkins ◽  
Dan Wang ◽  
Janet M. Wojcicki
Keyword(s):  
San Francisco ◽  
Low Income ◽  
Accelerated Aging ◽  
Positive Association ◽  
Dietary Factors ◽  
Sugar Sweetened Beverage ◽  
Protein Levels ◽  
Sweetened Beverages ◽  
Klotho Protein

Abstract Background Klotho is an anti-aging protein mainly expressed in the kidneys with a smaller amount expressed in adipose tissue. Klotho effects include roles in reducing oxidative stress, insulin signaling, adipogenesis and glucose metabolism. Few studies have investigated the role of dietary factors such as sugar sweetened beverages (SSBs) on serum α-klotho levels in young children. Methods Data was collected from 60 low-income Latina pregnant women and their infants in San Francisco from birth until 2 years of life and examined for associations between dietary factors and child secreted α-klotho protein levels at 2 years. Results Mean α-klotho levels were 1782.96 ± 874.56 pg/mL at 2 years of age. Any consumption of SSBs was independently associated with increased α-klotho levels (Beta = 682.79, 95%CI 67.50, 1298.09; p = 0.03). Household income ranging from $25,000 to $50,000 was also correlated to higher levels of α-klotho in children compared with lower income levels (<$25,000) (Beta = 1613.35, 95%CI 527.37, 2699.33; p = 0.005). Conclusions The positive association between SSB intake and α-klotho levels at 2 years may reflect higher phosphate levels consistent with SSB intake. Higher socioeconomic status may be a proxy for reduced stress exposure in children, also associated with higher α-klotho levels. Future studies should evaluate the early impact of exposures to SSBs, stress and accelerated aging in children.

Establishment of the Anti-Klotho Monoclonal Antibodies and Detection of Klotho Protein in Kidneys

2000 ◽  
Vol 267 (2) ◽  
pp. 597-602 ◽  
Author(s):  
Yukinari Kato ◽  
Emi Arakawa ◽  
Satoko Kinoshita ◽  
Akio Shirai ◽  
Akiko Furuya ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Klotho Protein

PHEX neutralizing agent inhibits dentin formation in mouse tooth germ

2011 ◽  
Vol 43 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Hongbing Lv ◽  
Sheng Fu ◽  
Gancha Wu ◽  
Fuhua Yan
Keyword(s):  
Tooth Germ ◽  
Neutralizing Agent ◽  
Dentin Formation

scholarly journals Regulation of the Voltage Gated K+Channel Kv1.3by Recombinant Human Klotho Protein

2014 ◽  
Vol 39 (6) ◽  
pp. 609-622 ◽  
Author(s):  
Ahmad Almilaji ◽  
Sabina Honisch ◽  
Guilai Liu ◽  
Bernat Elvira ◽  
Sumant Singh Ajay ◽  
...  
Keyword(s):  
K Channel ◽  
Voltage Gated ◽  
Klotho Protein

Local Irradiation of Mouse Tooth Germ Gives Insight into the Direct Effects of Irradiation on Root Development

2021 ◽  
Author(s):  
Yoshiaki Ide ◽  
Taka Nakahara ◽  
Tetsuya Fukada ◽  
Masanori Nasu
Keyword(s):  
Root Development ◽  
Root Formation ◽  
Tooth Germ ◽  
Lead Glass ◽  
Local Irradiation ◽  
Root Sheath ◽  
Calcified Tissue ◽  
Radiation Induced ◽  
Dentin Formation ◽  
Root Apices

To elucidate the mechanism underlying the failure of root formation after irradiation, we established a method of local irradiation of the molar tooth germ and demonstrated that radiation directly affected dental root development. In the current study, to locally irradiate the lower first molars of 5-day-old C57BL/6J mice, we used lead glass containing a hole as a collimator. We confirmed that our local irradiation method targeted only the tooth germ. The irradiated root was immature in terms of apical growth, and dentin formation was irregular along the outside of the root apices. Moreover, calcified tissue apically surrounded Hertwig's epithelial root sheath, which disappeared abnormally early. This method using a local irradiation experimental model will facilitate research into radiation-induced disorders of dental root formation.

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