Evaluation of an in vitro method for the measurement of specific IgE antibody responses: the rat basophilic leukemia (RBL) cell assay

Toxicology ◽  
2005 ◽  
Vol 206 (2) ◽  
pp. 195-205 ◽  
Author(s):  
R DEARMAN ◽  
R SKINNER ◽  
N DEAKIN ◽  
D SHAW ◽  
I KIMBER
Keyword(s):  
Specific Ige ◽  
Antibody Responses ◽  
In Vitro Method ◽  
Ige Antibody ◽  
Cell Assay ◽  
Basophilic Leukemia

Suppression of Specific IgE Antibody Responses by Liposome-Conjugated Ovalbumin in Mice Sensitized with Ovalbumin via the Respiratory Tract

2000 ◽  
Vol 121 (2) ◽  
pp. 108-115 ◽  
Author(s):  
Tomoki Yoshikawa ◽  
Tetsuya Uchida ◽  
Seishiro Naito ◽  
Atsuko Horino ◽  
Maiko Taneichi ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Specific Ige ◽  
Antibody Responses ◽  
Ige Antibody

Specific IgE antibody responses to ragweed allergens Ra5S and Ra5G associated with distinct HLA-DR β genes

1987 ◽  
Vol 24 (11) ◽  
pp. 1207-1210 ◽  
Author(s):  
K.M. Coulter ◽  
W.H. Yang ◽  
G. Dorval ◽  
M.A. Drouin ◽  
C.K. Osterland ◽  
...  
Keyword(s):  
Specific Ige ◽  
Antibody Responses ◽  
Ige Antibody ◽  
Hla Dr

Detection of Allergen-Specific IgE Antibody Responses

2005 ◽  
Vol 1 (3-4) ◽  
pp. 189-199 ◽  
Author(s):  
Petra A. Wachholz ◽  
Rebecca J. Dearman ◽  
Ian Kimber
Keyword(s):  
Specific Ige ◽  
Antibody Responses ◽  
Ige Antibody

Postmortem changes in hapten-specific IgE antibody responses in mice

1991 ◽  
Vol 104 (3) ◽  
pp. 133-135 ◽  
Author(s):  
Y. Heida ◽  
M. Kaguera ◽  
K. Hara ◽  
S. Kashimura
Keyword(s):  
Specific Ige ◽  
Antibody Responses ◽  
Postmortem Changes ◽  
Ige Antibody

scholarly journals Enriched-Baicalein Attenuates Allergy in Cells and Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mi-Young Yun ◽  
Ju-Im Jung ◽  
Sung-Min Park ◽  
Hwa-Jung Choi
Keyword(s):  
Immunoglobulin E ◽  
Inflammatory Cells ◽  
Specific Ige ◽  
Eosinophil Infiltration ◽  
Antiallergic Activity ◽  
Tryptase Level ◽  
Basophilic Leukemia

Enriched-baicalein (baicalein) from baicalin was prepared by fermentation of an SB extract with mycelium of Laetiporus sulphureus. To investigate the pharmacologic effects of baicalein, its antiallergic effect was measured in vitro and in vivo. Allergy was induced by intraperitoneal injection of ovalbumin (OVA) into Balb/c mice. As a result, baicalein showed antiallergic effects by inhibiting the release of β-hexosaminidase from immunoglobulin E- (IgE-) stimulated rat basophilic leukemia (RBL-2H3) mast cells without cytotoxicity after the methodology. After four weeks, the decrease of OVA-specific IgE level, decrease of histamine and tryptase level in serum, and then the decrease of the levels of T helper type 2 (Th2) cell-derived cytokines interleukin- (IL-) 4 and IL-13 in the splenocyte were observed. In a histological analysis for lung, baicalein excellently reduced eosinophil infiltration with the inhibition of characteristic lesions and inflammation including OVA-induced necrosis, numbers of inflammatory cells, and pulmonary edema. Therefore, these results showed that baicalein had excellent efficacy in the antiallergic activity.

scholarly journals Stimulation of primary in vitro IgE antibody responses in culture of human peripheral mononuclear cells.

1981 ◽  
Vol 153 (6) ◽  
pp. 1574-1581 ◽  
Author(s):  
M Nonaka ◽  
B L Zuraw ◽  
C H O'Hair ◽  
D H Katz
Keyword(s):  
Mononuclear Cells ◽  
Specific Ige ◽  
Regulatory Control ◽  
Pokeweed Mitogen ◽  
Ige Antibody ◽  
Protein Conjugates ◽  
Normal Humans ◽  
Peripheral Mononuclear Cells ◽  
Ige Mediated

Experiments are presented herein that demonstrate the capacity to stimulate human peripheral mononuclear cells to synthesize and secrete significant quantities of IgE molecules in vitro by exposure to appropriate concentrations of 2,4-dinitrophenyl (DNP)-protein conjugates, pokeweed mitogen (PWM), or a combination of DNP-proteins and PWM. Cultures stimulated in this fashion synthesize increased quantities of both total IgE and DNP-specific IgE antibody molecules. This in vitro human IgE antibody system should provide a useful tool for further exploration of regulatory control of IgE responses in both normal humans and those manifesting various forms of IgE-mediated allergic disorders.

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