DIAGNOSIS OF ALLERGY IN VITRO A COMPARISON BETWEEN SKIN SENSITIVITY TESTING AND SERUM LEVELS OF SPECIFIC IgE ANTIBODY IN CHILDREN

1973 ◽  
Vol 1 (26) ◽  
pp. 1293-1297 ◽  
Author(s):  
R. S. HOGARTH‐SCOTT ◽  
R. N. MCNICOL ◽  
H. E. WILLIAMS ◽  
S. G. O. JOHANSSON
Keyword(s):  
Serum Levels ◽  
Specific Ige ◽  
Sensitivity Testing ◽  
Skin Sensitivity ◽  
Ige Antibody

scholarly journals Stimulation of primary in vitro IgE antibody responses in culture of human peripheral mononuclear cells.

1981 ◽  
Vol 153 (6) ◽  
pp. 1574-1581 ◽  
Author(s):  
M Nonaka ◽  
B L Zuraw ◽  
C H O'Hair ◽  
D H Katz
Keyword(s):  
Mononuclear Cells ◽  
Specific Ige ◽  
Regulatory Control ◽  
Pokeweed Mitogen ◽  
Ige Antibody ◽  
Protein Conjugates ◽  
Normal Humans ◽  
Peripheral Mononuclear Cells ◽  
Ige Mediated

Experiments are presented herein that demonstrate the capacity to stimulate human peripheral mononuclear cells to synthesize and secrete significant quantities of IgE molecules in vitro by exposure to appropriate concentrations of 2,4-dinitrophenyl (DNP)-protein conjugates, pokeweed mitogen (PWM), or a combination of DNP-proteins and PWM. Cultures stimulated in this fashion synthesize increased quantities of both total IgE and DNP-specific IgE antibody molecules. This in vitro human IgE antibody system should provide a useful tool for further exploration of regulatory control of IgE responses in both normal humans and those manifesting various forms of IgE-mediated allergic disorders.

scholarly journals Peroxisome Proliferator–activated Receptors α and γ Down-regulate Allergic Inflammation and Eosinophil Activation

2003 ◽  
Vol 198 (3) ◽  
pp. 411-421 ◽  
Author(s):  
Gaetane Woerly ◽  
Kohei Honda ◽  
Marc Loyens ◽  
Jean-Paul Papin ◽  
Johan Auwerx ◽  
...  
Keyword(s):  
Airway Hyperresponsiveness ◽  
Allergic Inflammation ◽  
Serum Levels ◽  
Cell Types ◽  
Specific Ige ◽  
Peroxisome Proliferator ◽  
Inflammatory Reactions ◽  
Effector Cells ◽  
Peroxisome Proliferator Activated Receptors

Allergic asthma is characterized by airway hyperresponsiveness, eosinophilia, and mucus accumulation and is associated with increased IgE concentrations. We demonstrate here that peroxisome proliferator–activated receptors (PPARs), PPAR-α and PPAR-γ, which have been shown recently to be involved in the regulation of various cell types within the immune system, decrease antigen-induced airway hyperresponsiveness, lung inflammation, eosinophilia, cytokine production, and GATA-3 expression as well as serum levels of antigen-specific IgE in a murine model of human asthma. In addition, we demonstrate that PPAR-α and -γ are expressed in eosinophils and their activation inhibits in vitro chemotaxis and antibody-dependent cellular cytotoxicity. Thus, PPAR-α and -γ (co)agonists might be of therapeutic interest for the regulation of allergic or inflammatory reactions by targeting both regulatory and effector cells involved in the immune response.

Antigen-Specific IgE Antibody and Cytogram in Mucosa of the Nose and Sinus

1993 ◽  
Vol 7 (3) ◽  
pp. 111-115 ◽  
Author(s):  
Chia-Ming Liu ◽  
Chia-Tung Shun ◽  
Hue-Ching Song ◽  
Shiann-Yann Lee ◽  
Mow-Ming Hsu ◽  
...  
Keyword(s):  
Mast Cells ◽  
Plasma Cells ◽  
Chronic Sinusitis ◽  
Serum Levels ◽  
Specific Ige ◽  
Type I ◽  
Ige Antibody ◽  
Dust Mite ◽  
Sinus Mucosa ◽  
Maxillary Sinuses

Maxillary sinus mucosa from sinusitis patients (n = 60) and nasal mucosa from the inferior turbinates of hypertrophic rhinitis patients (n = 31) were collected to detect the appearance of tissue dust mite-specific IgE antibody and increase in eosinophils, mast cells, and plasma cells. The serum levels of total IgE and dust mite-specific IgE antibody were significantly greater in atopic patients than in nonatopic patients, whereas tissue specific IgE antibody was elevated in turbinate mucosa but not in sinus mucosa. Eosinophils and basophilic cells in epithelial scrapings from the inferior turbinates, assessed by Hansel stain, increased in about 85% of atopic patients with hypertrophic rhinitis (n = 16) and 60% of atopic patients with chronic sinusitis (n = 12). The increase in eosinophils, mast cells, and plasma cells was more prevalent in the turbinate mucosa of atopic patients than in that of nonatopics, but there was no such increase in sinus mucosa. We conclude that the maxillary sinuses may not be the target organ of type I allergic reaction and antigen-specific IgE antibody is probably produced by the local lymphoid tissue.

Association between Vitamins and Minerals with Antioxidant Effects and Coronary Artery Calcification in Adults and Older Adults: A Systematic Review

2019 ◽  
Vol 25 (22) ◽  
pp. 2474-2479 ◽  
Author(s):  
Alisson Diego Machado ◽  
Gustavo Rosa Gentil Andrade ◽  
Jéssica Levy ◽  
Sara Silva Ferreira ◽  
Dirce Maria Marchioni
Keyword(s):  
Systematic Review ◽  
Older Adults ◽  
Coronary Artery ◽  
Coronary Artery Calcification ◽  
Serum Levels ◽  
Antioxidant Compounds ◽  
Increased Risk ◽  
Coronary Events ◽  
Antioxidant Effects

Background: Coronary Artery Calcification (CAC) is considered an important cardiovascular risk factor. There is evidence that CAC is associated with an increased risk of atherosclerosis, coronary events and cardiovascular mortality. Inflammation is one of the factors associated with CAC and despite the interest in antioxidant compounds that can prevent CAC, its association with antioxidants remains unclear. Objective: This study aimed to systematically review the association between vitamins and minerals with antioxidant effects and CAC in adults and older adults. Methods: We conducted a systematic review using PubMed for articles published until October 2018. We included studies conducted in subjects aged 18 years and older with no previous cardiovascular disease. Studies involving animal or in vitro experiments and the ones that did not use reference methods to assess the CAC, dietary intake or serum levels of vitamin or mineral were excluded. Results: The search yielded 390 articles. After removal of duplicates, articles not related to the review, review articles, editorials, hypothesis articles and application of the inclusion and exclusion criteria, 9 articles remained. The results of the studies included in this systematic review suggest that magnesium is inversely associated with CAC and results on the association between CAC and vitamin E have been conflicting. Conclusion: Additional prospective studies are needed to elucidate the role of these micronutrients on CAC.

Sensitization to inhalant and food allergens in Brazilian atopic children by <i>in vitro</i> total and specific IgE assay. Allergy Project - PROAL

10.2223/1184 ◽  
2004 ◽  
Vol 80 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Charles K. Naspitz ◽  
Dirceu Solé ◽  
Cristina A. Jacob ◽  
Emanuel Sarinho ◽  
Francisco J. P. Soares ◽  
...  
Keyword(s):  
Specific Ige ◽  
Food Allergens
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