Development of a sensitive enzyme-linked immunosorbent assay for the detection of fumonisin B1 in maize

Toxicon ◽  
2012 ◽  
Vol 60 (7) ◽  
pp. 1245-1250 ◽  
Author(s):  
Yajie Sheng ◽  
Wenxiao Jiang ◽  
Sarah De Saeger ◽  
Jianzhong Shen ◽  
Suxia Zhang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Enzyme Linked Immunosorbent Assay for Fumonisin B1 Detection in Local Corn Seeds from Baghdad-Iraq

2021 ◽  
pp. 4621-4627
Author(s):  
Sinai W. Mohammed ◽  
Hanan J. Nayyef ◽  
Fadhaa O. Sameer ◽  
Ahmed Y. Hanoon
Keyword(s):  
Relative Density ◽  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fusarium Spp ◽  
Isolation And Identification ◽  
Toxic Compounds ◽  
Identification Of Fungi ◽  
Elisa Technique ◽  
Cancer Activity

Fungi produce a series of toxic compounds on corn, especially Fumonisin B1 (FB1) toxin produced by Fusarium spp. and promoting cancer activity in humans and animals. This study aimed to the isolation and identification of fungi associated with local corn seeds and the detection for the presence of FB1 by using ELISA technique. Thirty samples of corn ears were collected from silos and markets in Baghdad city during the period from November 2018 to March 2019. The present study found that Fusarium was the dominant isolate among fungi in terms of the relative density 57.07%, followed by Aspergillus 31.17%, Rhizopus 3.36%, Alternaria 2.88%, Mucor 2.16%, Penicillium 1.92%, Trichothecium 0.96%, and Helminthosporium 0.48%. FB1 was detected in all samples of the silos and markets with a concentration range of 13.69 - 175.54 µg/kg. There were no significant differences in FB1concentration among samples collected from the silos and markets. Also, no relationship was found between the number of infected seeds by Fusarium spp. and FB1concentrations.

An immunomagnetic-bead-based enzyme-linked immunosorbent assay for sensitive quantification of fumonisin B1

Food Control ◽  
2014 ◽  
Vol 40 ◽  
pp. 41-45 ◽  
Author(s):  
Yuan-Kai Wang ◽  
Yu-Chen Wang ◽  
Heng-an Wang ◽  
Wen-Hui Ji ◽  
Jian-He Sun ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunomagnetic Bead

A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin B1 in food samples

2006 ◽  
Vol 580 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Ying Quan ◽  
Yan Zhang ◽  
Shuo Wang ◽  
Nanju Lee ◽  
Ivan R. Kennedy
Keyword(s):  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

Molecularly imprinted polymer nanoparticle-based assay (MINA): application for fumonisin B1 determination

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3481-3488 ◽  
Author(s):  
Hasim Munawar ◽  
Katarzyna Smolinska-Kempisty ◽  
Alvaro Garcia Cruz ◽  
Francesco Canfarotta ◽  
Elena Piletska ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Molecularly Imprinted Polymer ◽  
Animal Feed ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Polymer Nanoparticle ◽  
Standard Tool

The enzyme-linked immunosorbent assay (ELISA) has been used as a standard tool for monitoring food and animal feed contamination from the carcinogenic fumonisin B1 (FB1).

Determination of hydrolysed fumonisin B1(HFB1) in corn by competitive direct enzyme‐linked immunosorbent assay

1996 ◽  
Vol 13 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Chris M. Maragos ◽  
Ronald D. Plattner ◽  
Steven D. Miklasz
Keyword(s):  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay

Immunizations and enzyme-linked immunosorbent assay (ELISA) for antibody isotype determination

2007 ◽  
Author(s):  
Stefan Wirtz ◽  
Clemens Neufert ◽  
Benno Weigmann ◽  
Markus F Neurath
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Isotype

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

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