scholarly journals Enzyme Linked Immunosorbent Assay for Fumonisin B1 Detection in Local Corn Seeds from Baghdad-Iraq

2021 ◽  
pp. 4621-4627
Author(s):  
Sinai W. Mohammed ◽  
Hanan J. Nayyef ◽  
Fadhaa O. Sameer ◽  
Ahmed Y. Hanoon
Keyword(s):  
Relative Density ◽  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fusarium Spp ◽  
Isolation And Identification ◽  
Toxic Compounds ◽  
Identification Of Fungi ◽  
Elisa Technique ◽  
Cancer Activity

Fungi produce a series of toxic compounds on corn, especially Fumonisin B1 (FB1) toxin produced by Fusarium spp. and promoting cancer activity in humans and animals. This study aimed to the isolation and identification of fungi associated with local corn seeds and the detection for the presence of FB1 by using ELISA technique. Thirty samples of corn ears were collected from silos and markets in Baghdad city during the period from November 2018 to March 2019. The present study found that Fusarium was the dominant isolate among fungi in terms of the relative density 57.07%, followed by Aspergillus 31.17%, Rhizopus 3.36%, Alternaria 2.88%, Mucor 2.16%, Penicillium 1.92%, Trichothecium 0.96%, and Helminthosporium 0.48%. FB1 was detected in all samples of the silos and markets with a concentration range of 13.69 - 175.54 µg/kg. There were no significant differences in FB1concentration among samples collected from the silos and markets. Also, no relationship was found between the number of infected seeds by Fusarium spp. and FB1concentrations.

scholarly journals Rapid Detection of Campylobacter coli, C. jejuni, and Salmonella enterica on Poultry Carcasses by Using PCR-Enzyme-Linked Immunosorbent Assay

2003 ◽  
Vol 69 (6) ◽  
pp. 3492-3499 ◽  
Author(s):  
Yang Hong ◽  
Mark E. Berrang ◽  
Tongrui Liu ◽  
Charles L. Hofacre ◽  
Susan Sanchez ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Salmonella Enterica ◽  
Rapid Detection ◽  
Diarrheal Disease ◽  
False Negative ◽  
Enzyme Linked Immunosorbent Assay ◽  
Campylobacter Coli ◽  
Culture Methods ◽  
Isolation And Identification ◽  
Serological Tests

ABSTRACT Contamination of retail poultry by Campylobacter spp. and Salmonella enterica is a significant source of human diarrheal disease. Isolation and identification of these microorganisms require a series of biochemical and serological tests. In this study, Campylobacter ceuE and Salmonella invA genes were used to design probes in PCR-enzyme-linked immunosorbent assay (ELISA), as an alternative to conventional bacteriological methodology, for the rapid detection of Campylobacter jejuni, Campylobacter coli, and S. enterica from poultry samples. With PCR-ELISA (40 cycles), the detection limits for Salmonella and Campylobacter were 2 � 102 and 4 � 101 CFU/ml, respectively. ELISA increased the sensitivity of the conventional PCR method by 100- to 1,000-fold. DNA was extracted from carcass rinses and tetrathionate enrichments and used in PCR-ELISA for the detection of Campylobacter and S. enterica, respectively. With PCR-ELISA, Salmonella was detected in 20 of 120 (17%) chicken carcass rinses examined, without the inclusion of an enrichment step. Significant correlation was observed between PCR-ELISA and cultural methods (kappa = 0.83; chi-square test, P < 0.001) with only one false negative (1.67%) and four false positives (6.67%) when PCR-ELISA was used to screen 60 tetrathionate enrichment cultures for Salmonella. With PCR-ELISA, we observed a positive correlation between the ELISA absorbance (optical density at 405 nm) and the campylobacter cell number in carcass rinse, as determined by standard culture methods. Overall, PCR-ELISA is a rapid and cost-effective approach for the detection and enumeration of Salmonella and Campylobacter bacteria on poultry.

Development of a sensitive enzyme-linked immunosorbent assay for the detection of fumonisin B1 in maize

Toxicon ◽  
2012 ◽  
Vol 60 (7) ◽  
pp. 1245-1250 ◽  
Author(s):  
Yajie Sheng ◽  
Wenxiao Jiang ◽  
Sarah De Saeger ◽  
Jianzhong Shen ◽  
Suxia Zhang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Isolation and Identification of Fungi Associated with the Spoilage of Sweet Orange and Banana in Sokoto Metropolis

2020 ◽  
Author(s):  
Michael Ameh
Keyword(s):  
Citrus Sinensis ◽  
Room Temperature ◽  
Sweet Orange ◽  
Potato Dextrose Agar ◽  
Test Results ◽  
Fusarium Spp ◽  
Isolation And Identification ◽  
Musa Sapientum ◽  
Identification Of Fungi ◽  
Pure Cultures

Mycological studies on Fungi in apparently diseased Sweet Orange ( Citrus sinensis) and Banana ( Musa sapientum) sampled from various points in Kara market in Sokoto Metropolis was carried out between August and September. The samples were surfaced sterilised with ethanol and the homogenates were cultured on Potato Dextrose Agar (PDA) and incubated aerobically at room temperature for 7 days at 30C. The pure cultures obtained were identified morphologically and microscopically. The investigation revealed that the samples were infected with several fungi species. The most predominant Fungi isolated from Sweet Orange were Cladosporium spp (40%), Fusarium spp (30%), Alternaria spp ( 20%), and Chrysonilia spp (10%) while the most predominant Fungi isolated from Banana were Fusarium spp (50%), Mucor spp ( 30%) and Rhizopus spp (20%). The pathogenecity test results show that Cladosporium spp and Fusarium spp were the most active in sweet orange with rot length of 74 mm and 70 mm respectively and the least active fungi were Alternaria spp and Chrysonilia spp with rot lengths of 52 mm and 48 mm respectively. Furthermore, Fusarium spp and Mucor spp were the most active in banana with rot lengths of 84 mm and 75 mm respectively and the least active fungus was Rhizopus spp with rot lengths of 54 mm.

scholarly journals Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline

2021 ◽  
Vol 5 (5) ◽  
pp. 447-453
Author(s):  
Rachmat Hidayat ◽  
Patricia Wulandari
Keyword(s):  
Immunosorbent Assay ◽  
Solid Surface ◽  
Color Change ◽  
Antibody Binding ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hormone Levels ◽  
Elisa Technique ◽  
Antibody Complex ◽  
Antigen Antibody ◽  
Detection Signal

ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

scholarly journals Comparison of Estrous Performance and Progesterone Level of Kacang Goats Induced by PGF2α Versus Ovsynch Protocol

2020 ◽  
Vol 151 ◽  
pp. 01045
Author(s):  
Budianto Panjaitan ◽  
Almira Dewi ◽  
Fadli FR Nasution ◽  
Mulyadi Adam ◽  
Tongku N. Siregar ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Immunosorbent Assay ◽  
Group Versus ◽  
Visual Observation ◽  
Progesterone Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Progesterone Concentration ◽  
Blood Samples ◽  
Elisa Technique ◽  
K2 Group

This study aimed to compare estrous performance and progesterone concentration during the estrous cycle of kacang goats induced by PGF2α versus ovsynch protocol. This research used six female kacang goats. The goats were divided into two groups namely the K1 group which was induced by injecting 7.5 mg PGF2α intramuscularly in 10 days interval and K2 group which was induced by using the ovsynch method. Protocol for K2 group was as follow; at day 1, the goats were injected with 7.5 mg PGF2α; at day 8, they were injected with 50 µg GnRH; at day 15 they were injected with 7.5 mg PGF2α; at day 18 they were injected with 50 µg GnRH. Estrous were detected using male goat and visual observation. The blood samples were taken on days 7, 14, and 21 after estrous. Progesterone concentration was measured with enzyme-linked immunosorbent assay (ELISA) technique. Intensity, onset, and duration of estrous in K1 group versus K2 group were 8.33±2.08 vs 7.00±1.00; 56.00±34.12 vs 36.00±20.78 hours; and 24.00±26.15 vs 24.00±20.78 hours, respectively (p>0.05). Level of progesterone hormone on day 7, 14, and 21 for K1 vs K2 were 0.812±0.710 vs 2.369±3.351; 5.051±7.754 vs 3.091±4.385 ng/ml; and 4.173±6.692 vs 3.562±4.113 ng/mL, respectively (p>0,05). It can be concluded that the differences in synchronization protocols between PGF2α versus ovsynch do not affect the performance of estrous and the concentration of progesterone during the estrous cycle of kacang goats.

Isolation and identification of associated fungi of Aspergillus and Penicillium genus and quantification of ochratoxin(A) in raisins samples in Tripoli Area (Preliminary study)

2020 ◽  
Vol 2 (1) ◽  
pp. 65-76
Author(s):  
Almahdi Ahmed Sassi ◽  
Sawsen Altaher Alfetouri ◽  
Altaher Ahmed Abouhleqa ◽  
Mohamed Ahmed Alryani ◽  
Ahmed Omran Tarsean
Keyword(s):  
Moisture Content ◽  
Ochratoxin A ◽  
Animal Feed ◽  
Average Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Isolation And Identification ◽  
Study Results ◽  
Elisa Technique ◽  
Codex Standard ◽  
Preliminary Study

This preliminary study aimed to determine the moisture content and isolation and identification of some fungi- genus of Aspergillus and Penicillium in 19 raisins samples (10 locals and 9 imported) randomly collected from different local markets in Tripoli city, Libya. The study included the detection and quantification of ochratoxin (A) that could be present in the selected raisins samples using enzyme-linked immunosorbent assay technique. Results indicated that the imported samples recorded the lowest moisture content vs. local samples (9.30% and 20.51%, respectively). Study results indicated that only two "%" local raisins samples having more moisture content than 18%. As far as fungi isolates, 76 fungi isolates were detected in all samples. Based on morphological and cultural characteristics, four species of such isolates belong to the genus Aspergillus while 3 species belong to the genus Penicillium. Using ELISA technique with a detection limit higher than 1.25 ng/gram, results revealed that 10 raisins samples (26.35%) contained ochratoxin (A) with an average concentration of 3.10 ng/gram. With the exception of two local c raisins samples that their moisture level was more than 18%, generally, results of this study were in confirm with Libyan Specification number 683-2013 set for the maximum permitted level of ochratoxin (A) in food and animal feed. In addition to codex standard number 67-1981

scholarly journals Cross-Reactivity of Fusarium spp. in the Aspergillus Galactomannan Enzyme-Linked Immunosorbent Assay

2011 ◽  
Vol 50 (3) ◽  
pp. 1051-1053 ◽  
Author(s):  
A. M. Tortorano ◽  
M. C. Esposto ◽  
A. Prigitano ◽  
A. Grancini ◽  
C. Ossi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fusarium Spp

The enzyme-linked immunosorbent assay (ELISA) test for the identification of blood-meals of haematophagous insects

1986 ◽  
Vol 76 (2) ◽  
pp. 321-330 ◽  
Author(s):  
M. W. Service ◽  
A. Voller ◽  
D. E. Bidwell
Keyword(s):  
Field Test ◽  
Immunosorbent Assay ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fresh Blood ◽  
Elisa Technique ◽  
Blood Meals

AbstractA sandwich enzyme-linked immunosorbent assay (ELISA) test was developed to detect blood-meals in insects and identify the host fed on. The test proved both sensitive and specific. Very small quantities of fresh blood (about 0·02 μl) can be detected; in practice, this enables blood in mosquitoes which are about three-quarters gravid to be identified. In trials in both Zambia and Britain, positive reactions were easily identified visually; consequently, this enabled the ELISA technique to be used as a routine field test. In addition to those of mosquitoes, blood-meals of a few Culicoides species were also successfully identified.

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