Development and validation of a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for the determination of the aflatoxin M1 levels in milk

Toxicon ◽  
2016 ◽  
Vol 113 ◽  
pp. 18-24 ◽  
Author(s):  
Dapeng Peng ◽  
Bijia Yang ◽  
Yuanhu Pan ◽  
Yulian Wang ◽  
Dongmei Chen ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Development And Validation

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

2012 ◽  
Vol 6 (3) ◽  
pp. 767-774 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Greta Nölke ◽  
Jing Zhang ◽  
Lanlan Niu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Matrices

Determination of Urinary Acetylpolyamines by a Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay (ELISA)

1995 ◽  
Vol 118 (6) ◽  
pp. 1211-1215 ◽  
Author(s):  
Kunio Fujiwara ◽  
Hiroshi Kanetake ◽  
Koichi Furukawa ◽  
Yukinobu Masuyama ◽  
Gang Bai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Determination of Homoharringtonine

Planta Medica ◽  
2018 ◽  
Vol 84 (14) ◽  
pp. 1038-1044 ◽  
Author(s):  
Benyakan Pongkitwitoon ◽  
Seiichi Sakamoto ◽  
Rika Nagamitsu ◽  
Waraporn Putalun ◽  
Hiroyuki Tanaka ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Myeloid Leukemia ◽  
High Performance ◽  
Sodium Periodate ◽  
Limit Of Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Support ◽  
Mediated Oxidation

AbstractHomoharringtonine (HHT), also known as omacetaxine, is a natural compound found in the genus Cephalotaxus and is a promising pharmaceutical drug used for the treatment of chronic or accelerated phase chronic myeloid leukemia. As a tool for the quantitative determination of HHT, a specific monoclonal antibody against HHT (MAb 6A1) was generated by conjugates prepared via sodium periodate-mediated oxidation. The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) using MAb 6A1 was found to be highly specific and sensitive with a limit of detection for HHT of 48.8 ng/mL. Validation assays to evaluate precision and accuracy of the method were conducted by the use of intra- and inter-assay analysis, recovery test, and comparison analysis between the amounts of HHT determined by ELISA and high-performance liquid chromatography. These results revealed that the established icELISA using MAb 6A1 is specific, sensitive, and reliable enough to be applied to the qualitative analysis for HHT. Furthermore, the results of this study support the usefulness of sodium periodate as a reagent for the conjugation between Cephalotaxus alkaloids and proteins for producing specific antibodies.

A Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Determination of Olaquindox in Animal Feed

2014 ◽  
Vol 47 (6) ◽  
pp. 1015-1030 ◽  
Author(s):  
Lei Wang ◽  
Jingyan Zhang ◽  
Dongan Cui ◽  
Xuezhi Wang ◽  
Zhiqiang Yang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Animal Feed ◽  
Enzyme Linked Immunosorbent Assay

Determination of trace aflatoxin M1 levels in milk and milk products consumed in Turkey by using enzyme-linked immunosorbent assay

2012 ◽  
Vol 25 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Sezgin Bakırdere ◽  
Tolga Yaroğlu ◽  
Nihan Tırık ◽  
Mehmet Demiröz ◽  
Abdullah Karaca
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk Products
Sign in / Sign up

Export Citation Format

Share Document