Synergistic effect of serine protease inhibitors and a bothropic antivenom in reducing local hemorrhage and coagulopathy caused by Bothrops jararaca venom

Toxicon ◽  
2021 ◽  
Author(s):  
G.M. Silva ◽  
D.H. Berto ◽  
C.A. Lima ◽  
K.B. Waitman ◽  
C.F.G. Lima ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Serine Protease ◽  
Protease Inhibitors ◽  
Serine Protease Inhibitors ◽  
Bothrops Jararaca

scholarly journals Comparison of Textilinin-1 with Aprotinin as Serine Protease Inhibitors and as Antifibrinolytic Agents

2005 ◽  
Vol 34 (4-5) ◽  
pp. 188-193 ◽  
Author(s):  
Simone Flight ◽  
Lambro Johnson ◽  
Manuela Trabi ◽  
Patrick Gaffney ◽  
Martin Lavin ◽  
...  
Keyword(s):  
Serine Protease ◽  
Protease Inhibitors ◽  
Serine Protease Inhibitors ◽  
Antifibrinolytic Agents

Examples of Peptide-Peptoid Hybrid Serine Protease Inhibitors Based on the Trypsin Inhibitor SFTI-1 with Complete Protease Resistance at the P1P1′ Reactive Site

ChemBioChem ◽  
2005 ◽  
Vol 6 (6) ◽  
pp. 1057-1061 ◽  
Author(s):  
Maciej Stawikowski ◽  
Roma Stawikowska ◽  
Anna Jaśkiewicz ◽  
Ewa Zabłotna ◽  
Krzysztof Rolka
Keyword(s):  
Serine Protease ◽  
Protease Inhibitors ◽  
Trypsin Inhibitor ◽  
Serine Protease Inhibitors ◽  
Reactive Site ◽  
Protease Resistance

Role of proteolysis in apoptosis: involvement of serine proteases in internucleosomal DNA fragmentation in immature thymocytes

1993 ◽  
Vol 71 (9-10) ◽  
pp. 488-500 ◽  
Author(s):  
Valerie M. Weaver ◽  
Boleslaw Lach ◽  
P. Roy Walker ◽  
Marianna Sikorska
Keyword(s):  
Molecular Weight ◽  
Serine Protease ◽  
High Molecular Weight ◽  
Protease Inhibitors ◽  
Dna Fragmentation ◽  
Dna Cleavage ◽  
Apoptotic Cell ◽  
Dependent Manner ◽  
Serine Protease Inhibitors ◽  
High Molecular Weight Dna

Three chemically distinct serine, but not cysteine, protease inhibitors (phenylmethylsulphonyl fluoride, N-tosyl-L-phenylalanylchloromethyl ketone and 3,4-dichloroisocoumarin) prevented, in a dose-dependent manner, the characteristic apoptotic internucleosomal DNA cleavage (DNA ladder) typically observed in thymocytes in response to dexamethasone and teniposide VM-26. This effect was not the result of a direct inhibition of the Ca2+, Mg2+-dependent endonuclease, since oligonucleosomal DNA cleavage occurred in the presence of these inhibitors in isolated nuclei. The proteolytic step occurred at a very early stage of apoptosis, and preincubation of thymocytes with the inhibitors before dexamethasone or teniposide VM-26 were added irreversibly suppressed ladder formation. This implied that the cellular effector(s) of these compounds preexisted and were not resynthesized in response to the inducers of apoptosis. Serine protease inhibitors also suppressed apoptotic cell shrinkage and complete nuclear collapse, suggesting that these morphological changes were directly related to internucleosomal fragmentation of DNA. However, the serine protease inhibitors did not prevent high molecular weight DNA cleavage (> 50 kilobases) that preceded the ladder formation and thymocytes still died by apoptosis. This supported the view that internucleosomal DNA cleavage, considered to be the biochemical marker of apoptosis, might in fact be a late and dispensable step and that the newly described high molecular weight DNA cleavage might be a better indicator of apoptosis.Key words: serine protease, apoptosis, internucleosomal DNA fragmentation, high molecular weight DNA cleavage, protease inhibitors.

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