Human neural progenitor cells as novel tool for in vitro DNT testing

2007 ◽  
Vol 172 ◽  
pp. S187
Author(s):  
Michaela Moors ◽  
Kathrin Gassmann ◽  
Jessica Heinrichs ◽  
Jason Cline ◽  
Thomas Rockel ◽  
...  
1999 ◽  
Vol 158 (2) ◽  
pp. 265-278 ◽  
Author(s):  
Melissa K. Carpenter ◽  
Xia Cui ◽  
Zhong-yi Hu ◽  
Jennifer Jackson ◽  
Sandy Sherman ◽  
...  

2020 ◽  
Vol 8 (2) ◽  
pp. 270 ◽  
Author(s):  
Rossella Gratton ◽  
Paola Maura Tricarico ◽  
Almerinda Agrelli ◽  
Heverton Valentim Colaço da Silva ◽  
Lucas Coêlho Bernardo ◽  
...  

The Zika virus (ZIKV) is an emergent arthropod-borne virus (arbovirus) responsible for congenital Zika syndrome (CZS) and a range of other congenital malformations. Evidence shows that ZIKV infects human neural progenitor cells (hNPCs) in the fetal brain, prompting inflammation and tissue damage/loss. Despite recent advances, little is known about the pathways involved in CZS pathogenesis. We performed a meta-analysis, gene ontology (GO), and pathway analysis of whole transcriptome studies with the aim of clarifying the genes and pathways potentially altered during hNPCs infection with ZIKV. We selected three studies (17 samples of infected hPNCs compared to hPNCs uninfected controls) through a systematic search of the Gene Expression Omnibus (GEO) database. The raw reads were trimmed, counted, and normalized. Next, we performed a rank product meta-analysis to detect consistently differentially expressed genes (DEGs) in these independent experiments. We detected 13 statistically significant DEGs. GO ontology and reactome analysis showed an enrichment of interferon, pro-inflammatory, and chemokines signaling and apoptosis pathways in ZIKV-infected cells. Moreover, we detected three possible new candidate genes involved in hNPCs infection: APOL6, XAF1, and TNFRSF1. Our results confirm that interferon (IFN) signaling dominates the ZIKV response, and that a crucial contribution is given by apoptotic pathways, which might elicit the CZS phenotype.


2005 ◽  
Vol 51 (2) ◽  
pp. 157-165 ◽  
Author(s):  
Haiyan Zhang ◽  
Yongmei Zhao ◽  
Chunli Zhao ◽  
Shuang Yu ◽  
Deyi Duan ◽  
...  

2004 ◽  
Vol 13 (5) ◽  
pp. 535-548 ◽  
Author(s):  
Ming Yang ◽  
Angela E. Donaldson ◽  
Cheryl E. Marshall ◽  
James Shen ◽  
Lorraine Iacovitti

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1486 ◽  
Author(s):  
Yury M. Lages ◽  
Juliana M. Nascimento ◽  
Gabriela A. Lemos ◽  
Antonio Galina ◽  
Leda R. Castilho ◽  
...  

Oxygen concentration should be carefully regulated in all living tissues, beginning at the early embryonic stages. Unbalances in oxygen regulation can lead to cell death and disease. However, to date, few studies have investigated the consequences of variations in oxygen levels for fetal-like cells. Therefore, in the present work, human neural progenitor cells (NPCs) derived from pluripotent stem cells grown in 3% oxygen (v/v) were compared with NPCs cultured in 21% (v/v) oxygen. Low oxygen concentrations altered the mitochondrial content and oxidative functions of the cells, which led to improved ATP production, while reducing generation of reactive oxygen species (ROS). NPCs cultured in both conditions showed no differences in proliferation and glucose metabolism. Furthermore, antioxidant enzymatic activity was not altered in NPCs cultured in 3% oxygen under normal conditions, however, when exposed to external agents known to induce oxidative stress, greater susceptibility to DNA damage was observed. Our findings indicate that the management of oxygen levels should be considered forin vitromodels of neuronal development and drug screening.


Sign in / Sign up

Export Citation Format

Share Document