Development of an in vitro test system to identify compounds with cell transforming activity

2009 ◽  
Vol 189 ◽  
pp. S67
Author(s):  
René Thierbach ◽  
Urte Blume ◽  
Pablo Steinberg
Keyword(s):  
Test System ◽  
In Vitro Test ◽  
Transforming Activity ◽  
Vitro Test ◽  
Cell Transforming

Development of an in-vitro test system for the evaluation of cyclooxygenase-2 inhibitors

1999 ◽  
Vol 48 (3) ◽  
pp. 133-138 ◽  
Author(s):  
S. Laufer ◽  
P. Zechmeister ◽  
T. Klein
Keyword(s):  
Test System ◽  
Cyclooxygenase 2 ◽  
In Vitro Test ◽  
Vitro Test ◽  
Cyclooxygenase 2 Inhibitors

An in vitro test system for compounds that modulate human inflammatory macrophage polarization

2018 ◽  
Vol 833 ◽  
pp. 328-338 ◽  
Author(s):  
Hiromi Shiratori ◽  
Carmen Feinweber ◽  
Sonja Luckhardt ◽  
Nadja Wallner ◽  
Gerd Geisslinger ◽  
...  
Keyword(s):  
Macrophage Polarization ◽  
Test System ◽  
In Vitro Test ◽  
Vitro Test ◽  
Inflammatory Macrophage

Biomimetic in vitro test system for evaluation of dental implant materials

2020 ◽  
Vol 36 (8) ◽  
pp. 1059-1070
Author(s):  
Franziska Ehlicke ◽  
Jonathan Berndt ◽  
Nina Marichikj ◽  
Doris Steinmüller-Nethl ◽  
Heike Walles ◽  
...  
Keyword(s):  
Dental Implant ◽  
Test System ◽  
In Vitro Test ◽  
Implant Materials ◽  
Vitro Test

scholarly journals Prediction of in Vivo Cytotoxicity of Chemotherapeutic Agents by Their Effect on Malignant Leukocytes in Vitro

Blood ◽  
1967 ◽  
Vol 30 (2) ◽  
pp. 176-188 ◽  
Author(s):  
MARTIN J. CLINE
Keyword(s):  
Test System ◽  
Chemotherapeutic Agents ◽  
Response To Treatment ◽  
In Vitro Test ◽  
Malignant Cells ◽  
Vitro Test ◽  
In Vitro Effects ◽  
In Vivo Cytotoxicity

Abstract In order to develop a test system for predicting the response to chemotherapeutic agents, leukocytes from patients with leukemia and leukolymphosarcoma were cultured in vitro and the effect of several drugs on the incorporation of H3-uridine into ribonucleic acid was measured. Cortisol, vincristine and cytosine arabinoside at concentrations near the therapeutic range produced inhibition of H3-uridine incorporation in sensitive leukocytes. The in vitro effects of 6-mercaptopurine and methotrexate were variable. In 39 trials on 25 patients with leukemia or lymphosarcoma, the in vitro test was used successfully to predict the response to treatment with prednisone and vincristine. It was concluded that the in vitro test system can predict the in vivo cytotoxicity of certain drugs for malignant cells, although it cannot be used to predict the likelihood of the induction of remissions with these drugs.

Retraction Of Fibrin Clots By Cultured Endothelial Cells

1981 ◽  
Author(s):  
B Barbieri ◽  
G Balconi ◽  
E Dejana ◽  
M B Donati
Keyword(s):  
Endothelial Cells ◽  
Primary Cultures ◽  
Fibrin Clot ◽  
Test System ◽  
In Vitro Test ◽  
Clot Retraction ◽  
System L ◽  
Vitro Test ◽  
Fibrin Clots

The interactions between endothelial cells (EC) and fibrin are still poorly understood. We approached this problem by studying the ability of cultured EC to induce in vitro the retraction of fibrin clots. EC were obtained from bovine aorta and human umbilical veins by collagenase treatment and grown in Eagle MEM. At the time of the test the cells were harvested from the flask by a short trypsin-EDTA treatment and resuspended in tyrode solution. The test system involved incubation of the cell suspension in a water-bath at 37°C in the presence of cell-free plasma which was clotted by thrombin. The course of retraction was followed by measuring the diameter of the clot with a microcaliper. Retraction values were expressed after calculation of percent activity by an appropriate formula. EC were found to induce the retraction of the fibrin clot to an extent which increased with the time (1-24 h) and with the number of cells in the system (l-4×l06/ml f.c.). Fibrin clot retractile (FCR) activity of EC could not be detected at 22°C or in presence of Na2-EDTA or using mechanically disrupted cells. Moreover, using batroxobin instead of thrombin as a clotting agent, no retraction occurred; FCR of EC thus showed many characteristics in common with platelet- and fibroblast- induced clot retraction.FCR activity of bovine EC increased with the number of subcultures, being very low in cells harvested from primary cultures. In contrast, human EC had high activity in primary cultures. Similarly to fibroblasts, EC with higher density in culture showed lower FCR, suggesting that con-fluency inhibits the cell contractile capacity.FCR could thus represent a simple in vitro test to further characterize the biology of EC and to evaluate their role in the development of fibrin thrombi.

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