Hexachlorobenzene induces cell proliferation and IGF-I signaling pathway in an estrogen receptor α-dependent manner in MCF-7 breast cancer cell line

2010 ◽  
Vol 192 (2) ◽  
pp. 195-205 ◽  
Author(s):  
María Alejandra García ◽  
Delfina Peña ◽  
Laura Álvarez ◽  
Claudia Cocca ◽  
Carolina Pontillo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Estrogen Receptor Α ◽  
Dependent Manner ◽  
Igf I ◽  
Mcf 7

scholarly journals An Antioxidant Polysaccharide from Ganoderma lucidum Induces Apoptotic Activity in Breast Cancer Cell Line 

2022 ◽  
Author(s):  
Md. Moyen Uddin Pk ◽  
Rumana Pervin ◽  
Mohammad Shahangir Biswas ◽  
Matiar Rahman
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Ganoderma Lucidum ◽  
Cancer Cell Line ◽  
Dependent Manner ◽  
Apoptotic Activity ◽  
Mcf 7

The purpose of this study is to elucidate the apoptotic activity of Ganoderma lucidum  polysaccharide (GLP) in a human breast cancer cell line MCF-7 in vitro.  According to DPPH assay, GLP showed a good antioxidant (IC 50  value is 202.4 µg/mL). Based on MTT assay, the results showed that GLP inhibits MCF-7 cells proliferation in a dose- and time- dependent manner (p<0.001). IC 50   values of the cytotoxicity of GLP and doxorubin  were 110.907 µg/mL and 58.206 µg/mL respectively. The results from the flow cytometry indicated that GLP could induce apoptotic activity through inducing the up-regulation of the Bax and Caspase-9 and the down-regulation of the BcL-2 in MCF-7 cells. At 2×IC 50 , GLP increased the early-apoptotic and dead cells of MCF-7 from 18.23% to 34.76% and 8.45% to16.34% respectively. In conclusion, the GLP shows anticancer activity against MCF-7 through preventing the proliferation and inducing the apoptosis of MCF-7 cells. Our data provide the potential molecular targets in cancer prevention and reveal the key barriers in the current anticancer drugs development.

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