An in-vitro validation study in the field of toxicokinetics and metabolism: Validation of an LC–MS/MS assay for simultaneous assessment of cytochrome P450 activity using cryopreserved human hepatocytes for compound-mediated induction of CYP3A4, CYP2B6, CYP2C9 and CYP1A2

2011 ◽  
Vol 205 ◽  
pp. S153
Author(s):  
E. Bourgogne ◽  
G. Saucez ◽  
S. Dell’Aiera ◽  
M. rosa ◽  
C. Jacques-hespel ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Validation Study ◽  
Human Hepatocytes ◽  
Cytochrome P450 Activity ◽  
P450 Activity ◽  
Simultaneous Assessment

scholarly journals In VitroAnalysis of the Interaction between Atovaquone and Proguanil against Liver Stage Malaria Parasites

2016 ◽  
Vol 60 (7) ◽  
pp. 4333-4335 ◽  
Author(s):  
Lídia Barata ◽  
Pascal Houzé ◽  
Khadija Boutbibe ◽  
Gigliola Zanghi ◽  
Jean-François Franetich ◽  
...  
Keyword(s):  
Plasmodium Yoelii ◽  
Human Hepatocytes ◽  
High Efficacy ◽  
Liver Stage ◽  
Content Type ◽  
Cytochrome P450 Activity ◽  
Main Target ◽  
P450 Activity ◽  
Pharmacological Basis

ABSTRACTThe interaction between atovaquone and proguanil has never been studied against liver stage malaria, which is the main target of this drug combination when used for chemoprevention. Using human hepatocytes lacking cytochrome P450 activity, and thus avoiding proguanil metabolizing into potent cycloguanil, we showin vitrothat the atovaquone-proguanil combination synergistically inhibits the growth of rodentPlasmodium yoeliiparasites. These results provide a pharmacological basis for the high efficacy of atovaquone-proguanil used as malaria chemoprevention.

In Vitro Cytochrome P450 Activity Decreases in Children with High Pediatric End-Stage Liver Disease Scores

2012 ◽  
Vol 41 (2) ◽  
pp. 390-397 ◽  
Author(s):  
Lies De Bock ◽  
Koen Boussery ◽  
Myriam Van Winckel ◽  
Peter De Paepe ◽  
Xavier Rogiers ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Liver Disease ◽  
End Stage Liver Disease ◽  
Cytochrome P450 Activity ◽  
End Stage ◽  
P450 Activity

Mediation of in Vitro Cytochrome P450 Activity by Common Pharmaceutical Excipients

2013 ◽  
Vol 10 (7) ◽  
pp. 2739-2748 ◽  
Author(s):  
Philip Martin ◽  
Marco Giardiello ◽  
Tom O. McDonald ◽  
Steven P. Rannard ◽  
Andrew Owen
Keyword(s):  
Cytochrome P450 ◽  
Pharmaceutical Excipients ◽  
Cytochrome P450 Activity ◽  
P450 Activity

Interspecies differences in the cytochrome P450 activity of hepatocytes exposed to PLGA and silica nanoparticles: an in vitro and in vivo investigation

Nanoscale ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 5171-5181 ◽  
Author(s):  
Raphaël Cornu ◽  
Nathalie Rougier ◽  
Yann Pellequer ◽  
Alf Lamprecht ◽  
Paul Hamon ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Silica Nanoparticles ◽  
Interspecies Differences ◽  
Hepatic Cells ◽  
Cytochrome P450 Activity ◽  
Set Up ◽  
P450 Activity

This study emphasizes the interest to set up relevant in vitro models using human hepatic cells to evaluate the hepatotoxicity of nanomedicines.

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