In Vitro Cytochrome P450 Activity Decreases in Children with High Pediatric End-Stage Liver Disease Scores

2012 ◽  
Vol 41 (2) ◽  
pp. 390-397 ◽  
Author(s):  
Lies De Bock ◽  
Koen Boussery ◽  
Myriam Van Winckel ◽  
Peter De Paepe ◽  
Xavier Rogiers ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Liver Disease ◽  
End Stage Liver Disease ◽  
Cytochrome P450 Activity ◽  
End Stage ◽  
P450 Activity

Effect of single-walled carbon nanotubes on cytochrome P450 activity in human liver microsomes in vitro

2018 ◽  
Vol 39 (5) ◽  
pp. 275-279
Author(s):  
Yuki Asai ◽  
Yukiko Sakakibara ◽  
Rina Inoue ◽  
Rikako Inoue ◽  
Masayuki Nadai ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Cytochrome P450 ◽  
Human Liver ◽  
Liver Microsomes ◽  
Single Walled Carbon Nanotubes ◽  
Single Walled Carbon ◽  
Cytochrome P450 Activity ◽  
Walled Carbon Nanotubes ◽  
P450 Activity

Mediation of in Vitro Cytochrome P450 Activity by Common Pharmaceutical Excipients

2013 ◽  
Vol 10 (7) ◽  
pp. 2739-2748 ◽  
Author(s):  
Philip Martin ◽  
Marco Giardiello ◽  
Tom O. McDonald ◽  
Steven P. Rannard ◽  
Andrew Owen
Keyword(s):  
Cytochrome P450 ◽  
Pharmaceutical Excipients ◽  
Cytochrome P450 Activity ◽  
P450 Activity

Repression of Cytochrome P450 Activity in Human Hepatocytes in Vitro by a Novel Hepatotrophic Factor, Augmenter of Liver Regeneration

2005 ◽  
Vol 316 (2) ◽  
pp. 822-829 ◽  
Author(s):  
Wolfgang E. Thasler ◽  
Rania Dayoub ◽  
Marcus Mühlbauer ◽  
Claus Hellerbrand ◽  
Thomas Singer ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Liver Regeneration ◽  
Human Hepatocytes ◽  
Augmenter Of Liver Regeneration ◽  
Cytochrome P450 Activity ◽  
P450 Activity

In vitro complex formation and inhibition of hepatic cytochrome P450 activity by different macrolides and tiamulin in goats and cattle

1999 ◽  
Vol 66 (1) ◽  
pp. 51-55 ◽  
Author(s):  
W.M ZWEERS-ZEILMAKER ◽  
A.S.J.P.A.M VAN MIERT ◽  
G.J HORBACH ◽  
R.F WITKAMP
Keyword(s):  
Cytochrome P450 ◽  
Complex Formation ◽  
Cytochrome P450 Activity ◽  
P450 Activity ◽  
Hepatic Cytochrome

Interspecies differences in the cytochrome P450 activity of hepatocytes exposed to PLGA and silica nanoparticles: an in vitro and in vivo investigation

Nanoscale ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 5171-5181 ◽  
Author(s):  
Raphaël Cornu ◽  
Nathalie Rougier ◽  
Yann Pellequer ◽  
Alf Lamprecht ◽  
Paul Hamon ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Silica Nanoparticles ◽  
Interspecies Differences ◽  
Hepatic Cells ◽  
Cytochrome P450 Activity ◽  
Set Up ◽  
P450 Activity

This study emphasizes the interest to set up relevant in vitro models using human hepatic cells to evaluate the hepatotoxicity of nanomedicines.

In Vitro Hepatic Differentiation of Adult, Embryonic, Induced Pluripotent and Perinatal Stem-Cells

2020 ◽  
Keyword(s):  
Stem Cells ◽  
Liver Transplantation ◽  
Liver Disease ◽  
Organ Transplantation ◽  
Cell Transplantation ◽  
Hepatic Differentiation ◽  
End Stage Liver Disease ◽  
Induced Pluripotent ◽  
End Stage

Globally regenerative medicine is considered as one of the rapidly growing biomedical industry have objective to substitute damaged cells. Cell transplantation is less intrusive than whole-organ transplantation, and has been used to provide an alternative for patients to whole-organ transplantation. The End-stage liver disease comprises a subgroup of patients with cirrhosis who have signs of decompensation that is irreversible with medical treatment. The only restorative therapy for severe end-stage liver disease is orthotropic liver transplantation. However, liver transplantation has several limitations such as scarcity of organ donors, immunosuppressive drugs, and several postoperative complications. Thus, cell transplantation can be used for the treatment of end stage liver disorders to decrease the mortality in acute liver failure. Therefore, stem cells can be used for cellular therapy, development of liver disease models, and tissue-engineering applications. This review involved the studies conducted on the stem cells potential of hepatic differentiation, isolated from different sources. The PubMed and Google Scholar were searched for scientific studies reported the sources of stem cells based on their origin and their potential of hepatic differentiation in-vitro by using different tools of differentiation. All the research articles were selected in which solely hepatic differentiation in combination with different tools is reported. Keywords: Adult Stem Cells, Embryonic Stem Cells, Induced Pluripotent Stem Cells, In-vitro, Mesenchymal Stem Cells.

The influence of glycosaminoglycans and crosslinking agents on the phenotype of hepatocytes cultured on collagen gels

2003 ◽  
Vol 22 (2) ◽  
pp. 65-71 ◽  
Author(s):  
Margarita Kataropoulou ◽  
Catherine Henderson ◽  
Helen Grant
Keyword(s):  
Cytochrome P450 ◽  
Laser Scanning ◽  
Cultured Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Collagen Gels ◽  
Sprague Dawley ◽  
Cytochrome P450 Activity ◽  
P450 Activity

The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture and the inability of the cells to proliferate. The aim of this study was to investigate the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v) and crosslinking the gels with either 1-ethyl-3-(3-di methylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression of glutathione-Stransferases (GSTs) and the activity of cytochrome P450 in hepatocytes cultured for 48 hours and 7 days. Hepatocytes were isolated from male Sprague–Dawley rats by collagenase perfusion. Cell homogenates were immunoblotted against class α and π GST subunits. To measure cytochrome P450 activity, testosterone hydroxylation was assessed. Viability of the cultured cells was assessed by confocal laser scanning microscopy using the vital stain carboxyfluorescein diacetate (CFDA). Cells cultured on gels crosslinked with EDAC were dead by 48 hours as judged by lack of CFDA-derived fluorescence and absence of GST bands on the immunoblots. The viability and morphology of the cells were unaffected by any of the other components of the substrata tested. Expression of GSTs indicated that the hepatocyte phenotype was stable for at least 48 hours. The addition of GAG did not improve the phenotype at either 48 hours or 7 days in culture, but the combination of GAG and DAH crosslinking improved GST expression in the 7-day cultures. However, the hepatocyte cytochrome P450 activity did not show any improvement on any of the gels. The combination of GAG and DAH crosslinking provided the most stable substratum environment in terms of GST expression in hepatocytes.

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