Single particle tracking method for the analysis of intracellular movement of the silica nanoparticles

2014 ◽  
Vol 229 ◽  
pp. S186-S187
Author(s):  
Michihiko Aoyama ◽  
Yasuo Yoshioka ◽  
Yoshiyuki Arai ◽  
Rio Ishimoto ◽  
Shin-ichi Tsunoda ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Tracking Method ◽  
Particle Tracking Method ◽  
Intracellular Movement

Single-Particle Tracking Method for Quantitative Tracking and Biophysical Studies of the MinE Protein

2008 ◽  
Vol 52 (3) ◽  
pp. 639-648 ◽  
Author(s):  
U. Junthorn ◽  
S. Unai ◽  
P. Kanthang ◽  
W. Ngamsaad ◽  
C. Modchang ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Tracking Method ◽  
Particle Tracking Method ◽  
Biophysical Studies

Local pH tracking in living cells

Nanoscale ◽  
2015 ◽  
Vol 7 (9) ◽  
pp. 4217-4225 ◽  
Author(s):  
Chieh-Jui Tsou ◽  
Chih-Hao Hsia ◽  
Jia-Yin Chu ◽  
Yann Hung ◽  
Yi-Ping Chen ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Particle Tracking ◽  
Single Particle ◽  
Mesoporous Silica Nanoparticles ◽  
Living Cells ◽  
Single Particle Tracking ◽  
Local Ph ◽  
Ph Detection

Simultaneous 3D single-particle tracking and local pH detection in living cells was achieved with ratiometric fluorescent mesoporous silica nanoparticles.

scholarly journals Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy

Entropy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 498
Author(s):  
Chen Zhang ◽  
Kevin Welsher
Keyword(s):  
Fisher Information ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Samples ◽  
Tracking System ◽  
Single Particle Tracking ◽  
Uniform Sampling ◽  
Single Dimension ◽  
3D Localization ◽  
Particle Localization

In this work, we present a 3D single-particle tracking system that can apply tailored sampling patterns to selectively extract photons that yield the most information for particle localization. We demonstrate that off-center sampling at locations predicted by Fisher information utilizes photons most efficiently. When performing localization in a single dimension, optimized off-center sampling patterns gave doubled precision compared to uniform sampling. A ~20% increase in precision compared to uniform sampling can be achieved when a similar off-center pattern is used in 3D localization. Here, we systematically investigated the photon efficiency of different emission patterns in a diffraction-limited system and achieved higher precision than uniform sampling. The ability to maximize information from the limited number of photons demonstrated here is critical for particle tracking applications in biological samples, where photons may be limited.

pH-Mediated nanoparticle dynamics in hydrogel nanocomposites

Soft Matter ◽  
2021 ◽  
Author(s):  
Katie A. Rose ◽  
Daeyeon Lee ◽  
Russell J. Composto
Keyword(s):  
Quantum Dot ◽  
Ethylene Glycol ◽  
Particle Tracking ◽  
Single Particle ◽  
Silica Particles ◽  
Single Particle Tracking ◽  
Poly Ethylene Glycol ◽  
Hydrogel Nanocomposites ◽  
Poly Ethylene ◽  
Nanoparticle Dynamics

The effect of static silica particles on the dynamics of quantum dot (QD) nanoparticles grafted with a poly(ethylene glycol) (PEG) brush in hydrogel nanocomposites is investigated using single particle tracking (SPT).

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