The THP-1 Toolbox: A new method that integrates the 4 key events of skin sensitization

2018 ◽  
Vol 295 ◽  
pp. S120
Author(s):  
E. Clouet ◽  
C. Raffalli ◽  
M.-H. Damiens ◽  
M. Pallardy ◽  
P.-J. Ferret ◽  
...  
Keyword(s):  
New Method ◽  
Skin Sensitization ◽  
Key Events

The THP-1 cell toolbox: a new concept integrating the key events of skin sensitization

2019 ◽  
Vol 93 (4) ◽  
pp. 941-951
Author(s):  
Elodie Clouet ◽  
Rami Bechara ◽  
Chloé Raffalli ◽  
Marie-Hélène Damiens ◽  
Hervé Groux ◽  
...  
Keyword(s):  
Skin Sensitization ◽  
Key Events

scholarly journals New Method for Monitoring Programmed Cell Death and Differentiation in Submerged Streptomyces Cultures

2010 ◽  
Vol 76 (10) ◽  
pp. 3401-3404 ◽  
Author(s):  
Paula Yagüe ◽  
Angel Manteca ◽  
Alejandro Simon ◽  
Marta Elena Diaz-Garcia ◽  
Jesus Sanchez
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Experimental Approach ◽  
Automatic Monitoring ◽  
New Method ◽  
Vital Stains ◽  
Key Events

ABSTRACT Vital stains were used in combination with fluorimetry for the elaboration of a new method to quantify Streptomyces programmed cell death, one of the key events in Streptomyces differentiation. The experimental approach described opens the possibility of designing online protocols for automatic monitoring of industrial fermentations.

scholarly journals Applicability of amino acid derivative reactivity assay for prediction of skin sensitization by combining multiple alternative methods to evaluate key events

2019 ◽  
Vol 44 (9) ◽  
pp. 585-600 ◽  
Author(s):  
Yusuke Yamamoto ◽  
Masaharu Fujita ◽  
Sayaka Wanibuchi ◽  
Ayako Sato ◽  
Miyuki Akimoto ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acid Derivative ◽  
Alternative Methods ◽  
Amino Acid Derivative ◽  
Skin Sensitization ◽  
Multiple Alternative ◽  
Key Events

scholarly journals In silico Prediction of Skin Sensitization: Quo vadis?

2021 ◽  
Vol 12 ◽  
Author(s):  
Giang Huong Ta ◽  
Ching-Feng Weng ◽  
Max K. Leong
Keyword(s):  
In Silico ◽  
Human Cell Line ◽  
Skin Area ◽  
Skin Sensitization ◽  
Animal Data ◽  
Cosmetic Ingredients ◽  
Quo Vadis ◽  
Allergic Contact ◽  
Key Events

Skin direct contact with chemical or physical substances is predisposed to allergic contact dermatitis (ACD), producing various allergic reactions, namely rash, blister, or itchy, in the contacted skin area. ACD can be triggered by various extremely complicated adverse outcome pathways (AOPs) remains to be causal for biosafety warrant. As such, commercial products such as ointments or cosmetics can fulfill the topically safe requirements in animal and non-animal models including allergy. Europe, nevertheless, has banned animal tests for the safety evaluations of cosmetic ingredients since 2013, followed by other countries. A variety of non-animal in vitro tests addressing different key events of the AOP, the direct peptide reactivity assay (DPRA), KeratinoSens™, LuSens and human cell line activation test h-CLAT and U-SENS™ have been developed and were adopted in OECD test guideline to identify the skin sensitizers. Other methods, such as the SENS-IS are not yet fully validated and regulatorily accepted. A broad spectrum of in silico models, alternatively, to predict skin sensitization have emerged based on various animal and non-animal data using assorted modeling schemes. In this article, we extensively summarize a number of skin sensitization predictive models that can be used in the biopharmaceutics and cosmeceuticals industries as well as their future perspectives, and the underlined challenges are also discussed.

Applicability of ADRA (4 mM) for the prediction of skin sensitization by combining multiple alternative methods to evaluate key events

2022 ◽  
Author(s):  
Mika Imamura ◽  
Yusuke Yamamoto ◽  
Masaharu Fujita ◽  
Sayaka Wanibuchi ◽  
Natsumi Nakashima ◽  
...  
Keyword(s):  
Alternative Methods ◽  
Skin Sensitization ◽  
Multiple Alternative ◽  
Key Events

Selective Sampling and Orientation of Non-Homogeneous Tissues

1968 ◽  
Vol 26 ◽  
pp. 98-99
Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Random Sampling ◽  
New Method ◽  
Microscope Examination ◽  
Small Areas ◽  
Selective Sampling ◽  
Large Numbers ◽  
Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

A new method for the determination of nitrates

1960 ◽  
Vol 23 ◽  
pp. 227-232 ◽  
Author(s):  
P WEST ◽  
G LYLES
Keyword(s):  
New Method
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