Applicability of ADRA (4 mM) for the prediction of skin sensitization by combining multiple alternative methods to evaluate key events

2022 ◽  
Author(s):  
Mika Imamura ◽  
Yusuke Yamamoto ◽  
Masaharu Fujita ◽  
Sayaka Wanibuchi ◽  
Natsumi Nakashima ◽  
...  
Keyword(s):  
Alternative Methods ◽  
Skin Sensitization ◽  
Multiple Alternative ◽  
Key Events

scholarly journals Applicability of amino acid derivative reactivity assay for prediction of skin sensitization by combining multiple alternative methods to evaluate key events

2019 ◽  
Vol 44 (9) ◽  
pp. 585-600 ◽  
Author(s):  
Yusuke Yamamoto ◽  
Masaharu Fujita ◽  
Sayaka Wanibuchi ◽  
Ayako Sato ◽  
Miyuki Akimoto ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acid Derivative ◽  
Alternative Methods ◽  
Amino Acid Derivative ◽  
Skin Sensitization ◽  
Multiple Alternative ◽  
Key Events

The THP-1 Toolbox: A new method that integrates the 4 key events of skin sensitization

2018 ◽  
Vol 295 ◽  
pp. S120
Author(s):  
E. Clouet ◽  
C. Raffalli ◽  
M.-H. Damiens ◽  
M. Pallardy ◽  
P.-J. Ferret ◽  
...  
Keyword(s):  
New Method ◽  
Skin Sensitization ◽  
Key Events

The THP-1 cell toolbox: a new concept integrating the key events of skin sensitization

2019 ◽  
Vol 93 (4) ◽  
pp. 941-951
Author(s):  
Elodie Clouet ◽  
Rami Bechara ◽  
Chloé Raffalli ◽  
Marie-Hélène Damiens ◽  
Hervé Groux ◽  
...  
Keyword(s):  
Skin Sensitization ◽  
Key Events

scholarly journals In silico Prediction of Skin Sensitization: Quo vadis?

2021 ◽  
Vol 12 ◽  
Author(s):  
Giang Huong Ta ◽  
Ching-Feng Weng ◽  
Max K. Leong
Keyword(s):  
In Silico ◽  
Human Cell Line ◽  
Skin Area ◽  
Skin Sensitization ◽  
Animal Data ◽  
Cosmetic Ingredients ◽  
Quo Vadis ◽  
Allergic Contact ◽  
Key Events

Skin direct contact with chemical or physical substances is predisposed to allergic contact dermatitis (ACD), producing various allergic reactions, namely rash, blister, or itchy, in the contacted skin area. ACD can be triggered by various extremely complicated adverse outcome pathways (AOPs) remains to be causal for biosafety warrant. As such, commercial products such as ointments or cosmetics can fulfill the topically safe requirements in animal and non-animal models including allergy. Europe, nevertheless, has banned animal tests for the safety evaluations of cosmetic ingredients since 2013, followed by other countries. A variety of non-animal in vitro tests addressing different key events of the AOP, the direct peptide reactivity assay (DPRA), KeratinoSens™, LuSens and human cell line activation test h-CLAT and U-SENS™ have been developed and were adopted in OECD test guideline to identify the skin sensitizers. Other methods, such as the SENS-IS are not yet fully validated and regulatorily accepted. A broad spectrum of in silico models, alternatively, to predict skin sensitization have emerged based on various animal and non-animal data using assorted modeling schemes. In this article, we extensively summarize a number of skin sensitization predictive models that can be used in the biopharmaceutics and cosmeceuticals industries as well as their future perspectives, and the underlined challenges are also discussed.

Compilation of Historical Local Lymph Node Data for Evaluation of Skin Sensitization Alternative Methods

Dermatitis ◽  
2005 ◽  
Vol 16 (04) ◽  
pp. 157 ◽  
Author(s):  
G. Frank Gerberick ◽  
Cindy A. Ryan ◽  
Petra S. Kern ◽  
Harald Schlatter ◽  
Rebecca J. Dearman ◽  
...  
Keyword(s):  
Lymph Node ◽  
Alternative Methods ◽  
Skin Sensitization ◽  
Local Lymph Node

scholarly journals Skin Sensitization Testing—What’s Next?

2019 ◽  
Vol 20 (3) ◽  
pp. 666 ◽  
Author(s):  
Gunilla Grundström ◽  
Carl Borrebaeck
Keyword(s):  
Predictive Accuracy ◽  
Machine Learning Techniques ◽  
Alternative Methods ◽  
Skin Sensitization ◽  
Animal Testing ◽  
Considerable Difficulty ◽  
Genomic Expression ◽  
High Information

There is an increasing demand for alternative in vitro methods to replace animal testing, and, to succeed, new methods are required to be at least as accurate as existing in vivo tests. However, skin sensitization is a complex process requiring coordinated and tightly regulated interactions between a variety of cells and molecules. Consequently, there is considerable difficulty in reproducing this level of biological complexity in vitro, and as a result the development of non-animal methods has posed a major challenge. However, with the use of a relevant biological system, the high information content of whole genome expression, and comprehensive bioinformatics, assays for most complex biological processes can be achieved. We propose that the Genomic Allergen Rapid Detection (GARD™) assay, developed to create a holistic data-driven in vitro model with high informational content, could be such an example. Based on the genomic expression of a mature human dendritic cell line and state-of-the-art machine learning techniques, GARD™ can today accurately predict skin sensitizers and correctly categorize skin sensitizing potency. Consequently, by utilizing advanced processing tools in combination with high information genomic or proteomic data, we can take the next step toward alternative methods with the same predictive accuracy as today’s in vivo methods—and beyond.

Compilation of Historical Local Lymph Node Data for Evaluation of Skin Sensitization Alternative Methods

Dermatitis ◽  
2005 ◽  
Vol 16 (4) ◽  
pp. 157-202 ◽  
Author(s):  
Frank G. Gerberick ◽  
Cindy A. Ryan ◽  
Petra S. Kern ◽  
Harald Schlatter ◽  
Rebecca J. Dearman ◽  
...  
Keyword(s):  
Lymph Node ◽  
Alternative Methods ◽  
Skin Sensitization ◽  
Local Lymph Node
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