Respiratory infections ofPseudomonas aeruginosaare a major cause of mortality and morbidity for hospitalized patients. Fine particulate matter (FPM) is known to have interactions with some bacterial infection in the respiratory system. In this report, we investigate the effect of different concentration of FPM onP. aeruginosaattachment and biofilm formation usingin vitrocell culture systems.P. aeruginosawere cultured to form mature biofilms on hydroxyapatite-coated peg and the number of bacteria in the biofilms was enumerated. Morphology of biofilm was imaged with scanning electron microscopy and confocal laser scanning microscopy. Bacterial affinity change to the cell membrane was evaluated with attached colony counting and fluorescence microscopy images. Alteration of bacterial surface hydrophobicity and S100A4 protein concentration were explored as mechanisms ofP. aeruginosaadhesion to human cells. There were a concentration-dependent increase of thickness and surface roughness of biofilm mass.P. aeruginosaadherence to respiratory epithelial cells was increased after FPM treatment. Bacterial surface hydrophobicity and S1000A4 protein concentration were increased with proportionally the dose of FPM in media. FPM in the airway could enhance both the adhesion ofP. aeruginosato epithelial cells and biofilm formation. Bacterial surface hydrophobicity and human cell plasma membrane injury are associated with binding ofP. aeruginosaon airway epithelial cells and biofilm formation.
Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells