A perspective on enantioselective chromatography by comparing ultra-high performance supercritical fluid chromatography and normal-phase liquid chromatography through the use of a Pirkle-type stationary phase

2021 ◽  
pp. 116511
Author(s):  
Giulia Mazzoccanti ◽  
Simone Manetto ◽  
Alessia Ciogli ◽  
Claudio Villani ◽  
Francesco Gasparrini
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
High Performance ◽  
Normal Phase ◽  
Enantioselective Chromatography ◽  
Phase Liquid

scholarly journals Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2425 ◽  
Author(s):  
Tsunehisa Hirose ◽  
Daniel Keck ◽  
Yoshihiro Izumi ◽  
Takeshi Bamba
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Retention Factor ◽  
Normal Phase ◽  
Retention Behavior ◽  
Selection Of

The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and π-π interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes.

Enantioseparation of Chiral Sulfoxides on Amylose-Based Columns: Comparison of Normal Phase Liquid Chromatography and Supercritical Fluid Chromatography

2017 ◽  
Vol 80 (4) ◽  
pp. 547-557 ◽  
Author(s):  
Natalie Kolderová ◽  
Tomáš Neveselý ◽  
Jiří Šturala ◽  
Martin Kuchař ◽  
Roman Holakovský ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Normal Phase ◽  
Chiral Sulfoxides ◽  
Phase Liquid
Sign in / Sign up

Export Citation Format

Share Document